STUDIES ON CHEMICAL AND PHYSICAL EFFECTS OF AIR POLLUTANTS ON AIRBORNE POLLEN IN URBANAREA OF JAPAN

日本市区空气污染物对空气中花粉的化学和物理影响研究

• 批准号: 17310031
• 负责人: WANG Qingyue
• 金额: $ 10.75万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17310031/
• 关键词: Japanese cedar pollen; Urban area; Air pollutants; Allergen particles; Size distribution; Collection of air samples; Analytical methods; アレルゲン; 微小化; 抽出法; 花粉; 寄与率

The purpose of this study is to evaluate the adverse factors influencing by air pollutants and to aid biomedical research by investigating the deposition of air pollutants on Japanese cedar pollen between urban and mountainous areas. Suspended particulate matter and Japanese cedar perigonium pollen were collected to identify the deposition of air pollutants at Saitama urban area and a mountainous area, during the pollination season. Ionic and metallic components of these samples and perigonium and pollen were analyzed. We found that crustal components (Al, Fe and Ti) tended to deposit on perigonium pollen. The amount of ionic components of particles (NO_3^-, SO_4^<2-> and NH_4^+) and gaseous pollutants (NO_2, SO_2 and NH_3) deposited on the perigonium pollen were higher in the urban than in the mountainous areas, the fact of which was proven by determination of gaseous pollutants exposed to Japanese cedar pollen. It is suggested that these phenomena may affect pollinosis and therefore … More it is necessary to investigate implication on biomedical researches.In addition, morphological observation of Ubisch bodies exfoliated from pollen and ruptured pollens was performed and confirmed with a scanning electron microscope. Moreover, the existing evidences of visualized Cry j 1 and Cry j 2 allergen particles below 1.1 μm were also sensitively found by an immunofluorescence technique with a fluorescence microscope. Simultaneously, the high concentrations of Cry j 1 and/or Cry j 2 were determined in the fine particle sizes below 1.1 μm. It was suggested that airborne respirable particulate matters containing Cry j 1 and Cry j 2 play a key role in the increasing incidence of asthma in the lower respiratory tract. Furthermore, since Cry j 1 (or Cry j 2) fine particles were suspended in the atmosphere over a long time more after released, the daily variation delays in the peaks of these concentrations compared with the peaks of airborne pollen counts were observed. It is important to observe and determine the size distribution of airborne Japanese cedar pollen allergen particles in the long-term sampling research continually, and to elucidate the allergen particle release mechanisms. Less

本研究的目的是通过调查城市和山区的空气污染物在日本柳杉花粉上的沉积情况，评估空气污染物的不利因素，并为生物医学研究提供帮助。采集悬浮颗粒物和日本柳杉花粉，以识别授粉季节埼玉市区和山区的空气污染物沉积情况。对这些样品以及周缘和花粉的离子和金属成分进行了分析。我们发现地壳成分（Al、Fe 和 Ti）倾向于沉积在perigium 花粉上。柳杉花粉中沉积的颗粒物离子成分(NO_3^-、SO_4^<2->和NH_4^+)和气态污染物(NO_2、SO_2和NH_3)的含量在城市地区高于山区,这一点通过对柳杉花粉中气态污染物的测定得到了证实。提示这些现象可能会影响花粉病，因此有必要探讨其对生物医学研究的意义。此外，还对花粉脱落的乌比施小体和花粉破裂的乌比施小体进行了形态学观察，并用扫描电子显微镜进行了证实。此外，通过荧光显微镜的免疫荧光技术，还灵敏地发现了1.1μm以下的可视化Cry j 1和Cry j 2过敏原颗粒的现有证据。同时，在低于1.1μm的细颗粒尺寸中测定了高浓度的Cry j 1和/或Cry j 2。研究表明，空气中含有Cry j 1和Cry j 2的可吸入颗粒物在下呼吸道哮喘发病率增加中发挥着关键作用。此外，由于Cry j 1（或Cry j 2）细颗粒在释放后更长时间地悬浮在大气中，因此观察到这些浓度峰值与空气中花粉计数峰值相比的每日变化延迟。在长期采样研究中持续观察和测定空气中柳杉花粉过敏原颗粒的粒径分布，对于阐明过敏原颗粒的释放机制具有重要意义。较少的

项目成果

スギ花粉アレルゲンCryj2含有粒子の粒径分布に関する研究

含雪松花粉过敏原Cryj2颗粒粒径分布研究

• 发表时间: 2007
• 期刊: 第24回エアロゾル科学・技術研究討論会論文集 1
• 作者: 栗原 幸大;王 青躍;坂本 和彦;三輪 誠;内山 巌雄
• 通讯作者: 内山 巌雄

Deposition of air pollutants on airborne Cryptomeria japonica pollen in urban area of Kanto Plain, Japan

日本关东平原市区空气污染物对柳杉花粉的沉积

• 发表时间: 2005
• 期刊: The Science and engineering reports of Saitama University 38
• 作者: Wang;Q.;Aoki;D.;Sakamoto;K.
• 通讯作者: K.

8th Meeting of the Palynological Society of Japan (Kurashiki, Japan)

日本孢粉学会第八次会议（日本仓敷）

• 发表时间: 2007
• 作者: Kurihara;K.;Wang;Q.;Kiryu;H.;Sakamoto;K.;Miwa;M.;Uchiyama;I.
• 通讯作者: I.

Measurement of polycyclic aromatic hydrocarbons in bio-diesel exhaustparticles

生物柴油尾气颗粒中多环芳烃的测量

• 发表时间: 2007
• 期刊: 2007 Proceedings of the 2007 International Symposium on Environmental Science and Technology (2007 ISEST)
• 作者: Shirai T.;Mogi F.;Sekiguchi K.;Wang Q.;Kurokawa H.;Yoshimura Y.;Sakamoto K.
• 通讯作者: Sakamoto K.

スギ花粉アレルゲン(Cry j 1およびCry j 2)含有粒子の粒径分布

含有雪松花粉过敏原的颗粒的粒径分布（Cry j 1和Cry j 2）

• 发表时间: 2007
• 作者: 栗原幸大;王青躍;坂本和彦;三輪誠;内山巌雄
• 通讯作者: 内山巌雄