Spatial Control of Bio-Materials by Suspended Carbon Nanotubes

本计画的目的分为两个主题：（1）控制碳奈米管网路朝向奈米积体元件，包括半导体、碳奈米管互连及生物功能元件。(2)生物分子在碳纳米管上的吸附机理研究，为碳纳米管在生物医学领域的应用奠定基础。为此，我们研究了碳纳米管三维网络的制备技术，以及吸附在碳纳米管表面的生物分子（如蛋白质和DNA）的表征。在碳纳米管网络中，我们已经开发了使用金属催化剂辅助的化学气相沉积的接枝技术。在该技术中，碳纳米管从沉积在饼生长的悬浮碳纳米管上的催化剂颗粒生长。使用这种技术，可以制造三维碳纳米管网络。结果发现，碳纳米管接枝是强大的观察生长机制，使用 ...更多信息 由于催化剂颗粒和生长的碳纳米管被固定在自由空间中，因此透射电子显微镜观察不到碳纳米管。DNA通过化学键固定在生长的碳纳米管片上。用荧光分子标记DNA并使用荧光显微镜观察。我们成功地观察到DNA在碳纳米管片上的吸附和DNA的杂交。蛋白质分子的吸附特性对于碳纳米管在生物技术和医学上的应用具有重要意义。我们将碳纳米管应用于血浆置换中的吸附材料，以去除引起肝脏疾病的胆红素。用红外振动光谱分析了吸附行为。我们发现蛋白质分子，如白蛋白和亲和素，在碳纳米管表面的吸附效率很高。综上所述，我们构建了三维碳纳米管网络，并将其应用于蛋白质和DNA分子的固定。少

The purpose of this project is classified into two themes:(1) Control of carbon nanotube networks toward nano-integrated devices including semiconductors, carbon nanotube interconnections and bio-functional components.(2) Investigation of adsorption mechanism of bio-molecules onto carbon nanotubes toward bio- and medical-application of carbon nanotubes.For these goals, we have studied fabrication techniques of carbon nanotube three-dimensional networks and characterization of bio-molecules, such as proteins and DNAs, adsorbed on carbon nanotube surfaces.In carbon nanotube networks, we have developed a grafting technique using a chemical vapor deposition assisted with metal catalysts. In this technique, carbon nanotubes are grown from catalyst particle deposited on the pie-grown suspended carbon nanotubes. Using this technique, a three-dimensional carbon nanotube networks can be fabricated. It was found that the carbon nanotube grafting is powerful to observe the growth mechanism using … More a transmission electron microscopy because the catalyst particles and grown carbon nanotubes are fixed in a free space.Carbon nanotube sheets were fabricated by the chemical vapor deposition using a porous silica film as a supporting material for catalyst particles. DNAs were immobilized on the grown carbon nanotube sheets through chemical bonds. DNAs were labeled with fluorescent molecules and observed using fluorescence microscopy. We succeeded to observe adsorption of DNAs onto the carbon nanotube sheets and hybridization of DNAs.Adsorption properties of protein molecules are important in application of carbon nanotubes to bio-technology and medicine. We applied carbon nanotubes to adsorption materials in plasma exchange to remove bilirubin which causes liver disease. The adsorption behavior was analyzed by infrared vibration spectroscopy. We found that protein molecules, such as albumin and avidin, are adsorbed on carbon nanotube surfaces at a high efficiency.In conclusion, we have fabricated three-dimensional carbon nanotube networks and applied them to immobilization of protein and DNA molecules. Less