Study on the flow behavior of DNA polymer dispersion

DNA聚合物分散体流动行为研究

• 批准号: 17360078
• 负责人: HASEGAWA Tomiichi
• 金额: $ 9.6万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17360078/
• 关键词: DNA Polymer; Biomaterial; Micro Flow; Elongational Flow; Fluid Engineering; 高分子構造; 伸長流; 物性; バイオテクノロジー

We have investigated elongational or aligning behaviors of DNA polymers in micro flows related to molecular manipulations for DNA analysis. Applicability of DNA polymer dispersions as a functional fluid is also examined in this project. Moreover, it was expected to obtain elemental data for the modeling of molecular motions in polymer dynamics simulations.In these view points, we have mainly conducted studies with micro flows. Deformation behaviors of DNA polymers in micro channels with an abrupt contraction, an L-shaped corner or a T-shaped joint have been directly observed using a fluorescence microscope. Various deformation or elongation patterns were clarified in such complex flow fields. It was also clarified that more effective extension of DNA polymers was obtained in combined elongational and shear flows than in purely elongational flows. Moreover, since the T-shaped joined channel generates a flow with higher elongational rate than that in the L-shaped corner flow, the T-shaped channel was helpful for the DNA polymer extension. The behaviors of DNA polymers in micro Couette flow were also observed with the same microscope. Polymer extending patterns in a start up flow and relaxation process of DNA polymers after cessation of the flow were observed. It is clarified that the length of DNA polymers decreases exponentially with time in the relaxation process.Macroscopic properties of DNA polymer solution were also measured utilizing rheometers with mechanical and optical devices. In the measurement of birefringence in steady shear flows, the increase in birefringence and decrease in orientation angle with shear rate increase were obtained. It is considered that these tendency would be related to the extension and alignment of DNA polymers.

我们研究了DNA聚合物在微流中的拉伸或排列行为，这些行为与DNA分析中的分子操作有关。DNA聚合物分散体作为功能流体的适用性也在这个项目中进行了检查。此外，在高分子动力学模拟中，还期望获得分子运动模型化所需的基础数据。基于这些观点，我们主要以微观流动为研究对象。利用荧光显微镜直接观察了DNA聚合物在具有突变收缩、L形拐角和T形接头的微通道中的变形行为。在这种复杂的流场中，各种变形或伸长模式被澄清。它也澄清了，更有效的延伸的DNA聚合物中获得的拉伸和剪切流相结合，而不是在纯粹的拉伸流。此外，由于T形连接通道产生比L形拐角流具有更高延伸速率的流，因此T形通道有助于DNA聚合物延伸。在同一显微镜下观察了DNA聚合物在微库埃特流中的行为。观察了启动流动中聚合物的延伸模式和流动停止后DNA聚合物的弛豫过程。结果表明，DNA聚合物的长度在松弛过程中随时间呈指数下降，并利用带有机械和光学装置的流变仪测量了DNA聚合物溶液的宏观性质。在定常剪切流中测量双折射时，得到了双折射随剪切速率的增加而增加，取向角随剪切速率的增加而减小的结果。据认为，这些趋势可能与DNA聚合物的延伸和排列有关。

项目成果

ミクロ流れ場でのDNA高分子の伸張変形

DNA聚合物在微流场中的伸长变形

• 发表时间: 2007
• 期刊: 日本機械学会2007年度年次大会講演論文集 Vol.2
• 作者: M.Oshima;R.Torii;小林 祐樹
• 通讯作者: 小林 祐樹

抵抗減少効果を示す液体の弾性力測定

测量液体弹力显示减阻效果

• 发表时间: 2006
• 期刊: 日本機械学会流体工学部門講演論文集 06-21
• 作者: M. Ouch;Y. Kobayashi;T. Narumi;T. Hasegawa;T. Takahashi;M.;高橋 正直
• 通讯作者: 高橋 正直

Sorimachi Elongational Deformation of DNA Polymers in Micro Channel Flows

DNA 聚合物在微通道流中的 Sorimachi 伸长变形

• 发表时间: 2007
• 期刊: Proc. Mechanical Engineering Congress, JSME Vol.2
• 作者: Y. Kobayashi;T. Narumi;T. Hasegawa;T. Takahashi;M. Shirakashi;K.
• 通讯作者: K.

Shirakashi Deformation Behavior of DNA Polymers in a Micro Channel

DNA 聚合物在微通道中的 Shirakashi 变形行为

• 发表时间: 2007
• 期刊: Proc. 55th the Rheology Symposium
• 作者: M. Ouch;Y. Kobayashi;T. Narumi;T. Hasegawa;T. Takahashi;M.
• 通讯作者: M.

DNA高分子のマイクロ流路内における変形挙動

DNA聚合物在微通道中的变形行为

• 发表时间: 2007
• 期刊: 第55回レオロジー討論会講演要旨集
• 作者: M.Oshima;R.Torii;小林 祐樹;大内 真由美
• 通讯作者: 大内 真由美