Bacterial Adhesion Inhibition and Biofilm Disruption by Adaptive Piezoelectric Biomaterial

自适应压电生物材料抑制细菌粘附和破坏生物膜

• 批准号: 10668030
• 负责人: Geelsu Hwang
• 金额: $ 20.78万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10668030
• 关键词: Acids; Address; Adhesions; Adhesives; Antimicrobial Resistance; Bacteria; Bacterial Adhesion; Barium; Binding; Biocompatible Materials; Bite Force; Candida albicans; Cells; Characteristics; Clinical; Composite Dental Resin; Data; Dental; Dental caries; Development; Electric Stimulation; Electron Transport; Electrostatics; Environment; Exhibits; Failure; Fluorescence; Future; Goals; Histologic; Homeostasis; Human; Hydrophobicity; Impairment; In Vitro; Incubated; Individual; Infection; Long-Term Effects; Mastication; Measures; Methodology; Microbe; Microbial Biofilms; Microfluidics; Modality; Modeling; Motion; Oral; Oral cavity; Outcome; Performance; Population; Prevention strategy; Property; Risk Reduction; Rodent; Rodent Model; Streptococcus gordonii; Streptococcus mutans; Surface; Surface Properties; System; Testing; Thick; Tooth Tissue; Tooth structure; Toothbrushing; Work; bactericide; biomaterial compatibility; clinical application; clinically relevant; cost comparison; cytotoxicity; design; efficacy evaluation; electrical potential; electrical property; experimental study; functional restoration; fungus; in vivo; in vivo Model; innovation; mathematical model; mechanical force; mechanical properties; metallicity; microbial; microbial colonization; microbiota; microleakage; microorganism; nanocomposite; nanoparticle; novel; oral microbial community; pathogenic bacteria; polymicrobial biofilm; prevent; real-time images; response; restoration; restorative material; zeta potential

Dental resin composites have been widely used clinically due to their bonding potential to the tooth tissues, good mechanical properties, and lower cost compared to other indirect restorations. While successful, long-term survival of a restoration can be compromised by secondary caries at the tooth-composite margins. In most cases, failure is due to the microleakage of bacteria and their acid by-products through the margins between composite and tooth structures. Once biofilms are established on a surface, it is extremely difficult to remove or kill pathogenic bacteria therein. Therefore, inhibition of microbial adhesion or inactivation of the adhered bacteria could impair their development into biofilms. The goal of this application is to create a novel dental composite that inhibits biofilm accumulation as well as dislodging surface-adhered microbes on restorative materials using enhanced electric potential at the interface generated by oral motion without relying on microbial killing activity. A nanocomposite platform based on barium titanate (BaTiO3) nanoparticles enables antibiofilm and self-powering functionalities for biomedical applications. This nanocomposite surface inhibits bacterial colonization by utilizing its intrinsic physicochemical properties without bactericidal activity, thereby minimizing the induction of antimicrobial resistance and destruction of homeostasis microbiota. In addition, the piezoelectric property of BaTiO3 nanoparticles that converts normal oral motions into electrical energy can be utilized to enhance its antibiofilm activity. Ongoing studies indicate that antibiofilm activity can be further enhanced by modulating the work function by introducing a shallow metallic surface (< 100 Å) on the nanocomposite, exhibiting almost complete inhibition of bacterial colonization. Based on this exciting supporting data, we hypothesize that force- powering of piezoelectric crystals to produce enhanced electric potential combined with bacterial anti-adhesive property creates an anti-infectious environment that prevents the development of biofilms on restorations and secondary caries. We anticipate that the creation of this anti-infectious smart biomaterial would increase the functionality of restorations and provide a new strategy to prevent secondary caries as well as reduce the risk of restoration failure.

牙科树脂复合材料由于其与牙体组织的粘接能力好， 机械性能和成本较低。虽然成功，但长期 修复体的存活可能受到牙齿复合物边缘处的继发性龋齿的影响。在大多数情况下， 失效是由于细菌及其酸性副产物通过复合材料之间的边缘微渗漏 牙齿结构。一旦生物膜在表面上建立，就极难去除或杀死 其中的致病菌。因此，抑制微生物粘附或灭活粘附的细菌 会阻碍它们形成生物膜该应用的目标是创造一种新型牙科复合材料 抑制生物膜的积累，以及驱逐表面附着的微生物的修复材料， 增强口腔运动产生的界面处的电势，而不依赖于微生物杀灭活性。 基于钛酸钡（BaTiO 3）纳米颗粒的纳米复合材料平台可实现镀膜和自供电 生物医学应用的功能。这种纳米复合材料表面通过利用 其固有的物理化学性质没有杀菌活性，从而最大限度地减少诱导 抗微生物剂耐药性和稳态微生物群的破坏。此外， 将正常口腔运动转化为电能的BaTiO 3纳米颗粒可用于增强其 电影活动。正在进行的研究表明，通过调节细胞膜活性， 通过在纳米复合材料上引入浅金属表面（< 100 μ m）， 完全抑制细菌定植。基于这些令人兴奋的支持数据，我们假设力- 为压电晶体供电以产生与细菌抗粘附剂结合的增强的电势 该特性创造了一种抗感染环境，可防止微生物上生物膜的发展， 继发龋我们预计，这种抗感染智能生物材料的创造将增加 提供了一种新的策略，以防止继发性龋，以及减少风险， 恢复失败。