Microfluidic Devices for the analysis of protein functions with monodisperse liposomes

用单分散脂质体分析蛋白质功能的微流体装置

• 批准号: 17360111
• 负责人: TAKEUCHI Shoji
• 金额: $ 9.98万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17360111/
• 关键词: Liposome; MEMS; microTAS; Microfluidic devices; マイクロフルイディスク

In this research, we have studied a device for the efficient production of monodisperse liposomes. To prepare the liposomes, we have investigated two different methods : electroformation and conventional gentle-hydration method. We found that the liposomes produced by the conventional method were relatively small size, and several liposomes were enclosed inside another liposome. On the other hand, the liposomes formed by electroformation were mostly giant liposomes and did not enclose liposomes inside.Through these results, we have tried to make monodisperse liposomes by using microfluidic devices for emulsification, towards new technology of liposome production. It is general to change the wetting properties of the microfluidic channel for making W/O/W emulsions. Here, we have designed and fabricated axisymetric flow-focusing devices, and succeeded in making W/O/W emulsions. This method can be useful for making lipid bilayer vesicles by thinning the layer of organic solvent. Moreover, microfluidic devices are suitable for making monodisperse vesicles. We thus believe that this technology could be a route to the production of monodisperse liposomes.

在本研究中，我们研究了单分散脂质体的高效生产装置。为了制备脂质体，我们研究了两种不同的方法：电铸法和传统的温和水合法。我们发现用常规方法制备的脂质体体积相对较小，并且几个脂质体被包裹在另一个脂质体中。另一方面，电成型法制备的脂质体多为大粒径的脂质体，内部没有包裹脂质体，因此，我们尝试利用微流控装置进行乳化，制备单分散脂质体，为脂质体的制备开辟新的技术途径。为了制备W/O/W乳液，通常改变微流体通道的润湿性质。在此，我们设计并制作了轴对称流动聚焦装置，并成功地制备了W/O/W型乳剂。该方法可用于通过使有机溶剂层变薄来制备脂质双层囊泡。此外，微流体装置适合于制备单分散囊泡。因此，我们相信，这种技术可能是一种生产单分散脂质体的途径。

项目成果

A monolithically three-dimensional flow-focusing device for formation of single/double emulsions in the closed/open microfluidic systems

一种用于在封闭/开放微流体系统中形成单/双乳液的整体式三维流动聚焦装置

• 发表时间: 2006
• 期刊: Journal of Micromechanics and Microengineering 16
• 作者: Shih-Hao Huang;Wei-Heong Tan;Fan-Gang Tseng;Shoji Takeuchi
• 通讯作者: Shoji Takeuchi

Systhesis of Bio-Functionalized Copolymer Particles in 3D Microfluidic Devices

3D 微流控装置中生物功能化共聚物颗粒的合成

• 发表时间: 2007
• 期刊: MEMS2007
• 作者: S-H.Huang;W.H.Tan;F.G.Tseng;S.Takeuchi
• 通讯作者: S.Takeuchi

A Monolithic 3D Flow-Focusing Device for the Formation of Single/Double Emulsions in a Closed/Open Microfluidic System

用于在封闭/开放微流体系统中形成单/双乳液的整体 3D 流动聚焦装置

• 发表时间: 2006
• 期刊: microTAS2006
• 作者: S.H.Huang;W.H.Tan;F.G.Tseng;S.Takeuchi
• 通讯作者: S.Takeuchi

Utilization of Cell-Sized Lipid Containers for Nanostructures and Macromolecules Handling in Microfabricated Devices

利用细胞大小的脂质容器进行微加工器件中的纳米结构和高分子处理

• 发表时间: 2005
• 期刊: Analytical Chemistry 77
• 作者: Guillaume Tresset;Shoji Takeuchi
• 通讯作者: Shoji Takeuchi

Preparation of Monodisperse Giant Liposomes in Micro-Fluidic Channels (in Japanese)

微流道中单分散巨型脂质体的制备（日文）

• 发表时间: 2006
• 期刊: H18 Sensor and Micromachine Sogo Kenkyu-kai
• 作者: K.Kuribayashi;S.Takeuchi
• 通讯作者: S.Takeuchi