Creation Technology of Nano-mechanisms using Particle Positioning on DNA Strands

在本研究中，研究了利用DNA和相关生物技术创建纳米机制的新技术，并获得了以下结果：通过DNA周期性构建纳米机制：纳米颗粒修饰的DNA双链片段已经被创建并连接（通过酶相互连接）了几十个区块。由于DNA核酸的间距为0.34nm，因此与机械获得的定位分辨率相比，定位分辨率非常高。实验结果表明，通过优化核酸序列得到了周期性的纳米结构，并通过原子力显微镜（AFM.2）进行了观察。利用蛋白质复合物建立分子纳米机制：为了增加致动器和传感器等功能，提出了一种由DNA和蛋白质气体组成的复合物。一种叫做IHF的DNA折叠蛋白被引入到指定的核酸序列上，并通过AM显微照片实验证实了这一点。同时，在杂交序列的分支DNA上引入了ReccA蛋白，实验证实其为纳米连接。环形DNA的纳米结构和DNA的力学性能评价：提出并实验制备了三种类型的纳米结构：DNA玻璃、序列DNA环和DNA链。形成了直径为27nm的DNA玻璃，并经原子力显微镜证实。用螺旋弹簧作为DNA的主结构，用小弹簧作为核酸和氢键，建立了DNA的力学模型，并与实验结果进行了比较，结果吻合较好。

In this research, a new technology for creation of nano-mechanisms using DNA and related biotechnology has been investigated and obtained the following results.1.Periodic construction of nano-mechanisms by DNA: Nano-particle modified DNA double strand fractions have been created and ligated (connected each other by enzyme) for several tens of blocks. Since the pitch of the DNA nucleic acid is 0.34nm, the positioning resolution is very fine compare to those of mechanically obtained. Experimental results show the periodic nano-structure by the optimization of the sequence of the nucleic acid, which are observed by the AFM.2.Creation of Molecular nano-mechanisms by protein complex: To add the function such as actuators and sensors, a complex with DNA and protein gas been proposed. A DNA folding protein called IHF has been introduced to bend the DNA at designated nucleic sequence, and experimentally confirmed by AM micrographs. Also, a protein ReccA has been introduced to branch DNA at the hybridized sequence, which is experimentally confirmed as nano-junctions.3.Nano-structures by circular DNA and Mechanical Property Evaluation of DNA : Three types of nano-structure, DNA glasses, Serial DNA rings, and DNA chain has been proposed and experimentally fabricated. A DNA glasses with a diameter of 27nm has been formed and confirmed by AFM. A mechanical model of the DNA has been proposed by combining coil spring for main structure and small springs for nucleic and hydrogen bonding, which is compared with the experimental results and show good agreement.