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Creation Technology of Nano-mechanisms using Particle Positioning on DNA Strands

利用 DNA 链上的粒子定位的纳米机制创建技术

基本信息

批准号：
17360112
负责人：
HATSUZAWA Takeshi
金额：
$ 10.16万
依托单位：
Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17360112/
关键词：
DNA Nano-Machine Nano-Bio Molecular Mechanism

项目摘要

In this research, a new technology for creation of nano-mechanisms using DNA and related biotechnology has been investigated and obtained the following results.1.Periodic construction of nano-mechanisms by DNA: Nano-particle modified DNA double strand fractions have been created and ligated (connected each other by enzyme) for several tens of blocks. Since the pitch of the DNA nucleic acid is 0.34nm, the positioning resolution is very fine compare to those of mechanically obtained. Experimental results show the periodic nano-structure by the optimization of the sequence of the nucleic acid, which are observed by the AFM.2.Creation of Molecular nano-mechanisms by protein complex: To add the function such as actuators and sensors, a complex with DNA and protein gas been proposed. A DNA folding protein called IHF has been introduced to bend the DNA at designated nucleic sequence, and experimentally confirmed by AM micrographs. Also, a protein ReccA has been introduced to branch DNA at the hybridized sequence, which is experimentally confirmed as nano-junctions.3.Nano-structures by circular DNA and Mechanical Property Evaluation of DNA : Three types of nano-structure, DNA glasses, Serial DNA rings, and DNA chain has been proposed and experimentally fabricated. A DNA glasses with a diameter of 27nm has been formed and confirmed by AFM. A mechanical model of the DNA has been proposed by combining coil spring for main structure and small springs for nucleic and hydrogen bonding, which is compared with the experimental results and show good agreement.

本研究利用DNA及相关生物技术构建纳米结构，取得了以下成果：1. DNA周期性构建纳米结构：构建了纳米粒子修饰的DNA双链片段，并将其连接（通过酶相互连接）成数十个片段。由于DNA核酸的间距为0.34nm，因此与机械获得的定位分辨率相比，定位分辨率非常精细。实验结果表明，通过对核酸序列的优化，可以得到周期性的纳米结构，并通过AFM观察到。2.利用蛋白质复合物构建分子纳米机构：为了增加致动器和传感器等功能，提出了一种含有DNA和蛋白质气体的复合物。一种称为IHF的DNA折叠蛋白被引入到指定的核酸序列处使DNA弯曲，并通过AM显微照片进行了实验证实。此外，蛋白质ReccA已被引入到杂交序列处的分支DNA中，这被实验证实为纳米结。3.环状DNA的纳米结构和DNA的机械性能评价：提出并实验制备了三种类型的纳米结构，DNA玻璃，串联DNA环和DNA链。用原子力显微镜（AFM）证实了DNA玻璃的直径为27nm。提出了以螺旋弹簧为主体结构，以小弹簧为氢键和核酸键合的DNA力学模型，并与实验结果进行了比较，两者吻合较好。