Regulation of cell differentiation and stimulation on D-aglucse displayed dendrimer surface

已知锚定依赖细胞与底物的黏附调节多种细胞事件，如信号、基因表达和蛋白发生变化。特别是，整合素与细胞配体的结合会导致细胞黏附、形状改变和迁移等功能后果。这对几个细胞物种的细胞功能至关重要。因此，这些用于细胞黏附的底物的开发可以为细胞生物学提供新的见解，并为控制培养细胞和组织的形态发生提供复杂的方法学。本项目包括三个部分：培养表面的设计和表征，作为基础研究的细胞在D-葡萄糖显示表面的附着和形态的理解，以及它在细胞功能方面的应用。第一部分为表面设计与表征。第二部分包括后两个主题，涉及基于葡萄糖显示表面的形态变化机制的阐明，葡萄糖显示表面上皮细胞的形态变化，软骨细胞的形态调控，以及随后在葡萄糖显示表面的软骨细胞表达。在论文的最后部分，我们提出了培养表面产生的新功能，如靶向性和对葡萄糖展示表面的粘附性，将为组织构建提供一种新的信号转导系统。

Adhesion of anchorage-dependent cells to underlying substrate is known to modulate a variety of cellular events such as signaling, gene expression and proteogenic changes. In particular, integrin bindings to cellular ligands results in functional consequences such as cell adhesion, shape change, and migration. that is crucial to cellular functions in several cell species. Thus, the development of these substrates for cell adhesion can provide new insights into cell biology as well as sophisticated methodology for controlling morphogenesis of cultured cells and tissues.This project consisted of three parts; the design of culture surface and characterization, understanding of cell attachment and morphology on D-glucose-displayed surface as the fundamental study, and its application for the cell functions. The first part covered the surface design and characterization. The second part which contained the last two themes deals with the elucidation of mechanism of morphological changes based on glucose-displayed surface; morphological changes of epithelial cells on glucose-displayed surface, morphological regulation of chondrocytes, and subsequently, chondrogenic expression on glucose-displayed surface. In the last part, we proposed that new function arising from culture surface such as targetability and adhesion to glucose-displayed surface could introduce a novel signal-transduction system to be applied for tissue construction.