Regulation of cell differentiation and stimulation on D-aglucse displayed dendrimer surface

D-alucse 展示的树枝状聚合物表面上细胞分化和刺激的调节

• 批准号: 17360398
• 负责人: KINO-OKA Masahrio
• 金额: $ 7.46万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17360398/
• 关键词: Biotechnology and Bionics; Cell and tissue; Regenerative medicine; Desien of culture surface; regulation of cell differentiation; Chondrocyte cell; Epithelial cell; D-glucose display

Adhesion of anchorage-dependent cells to underlying substrate is known to modulate a variety of cellular events such as signaling, gene expression and proteogenic changes. In particular, integrin bindings to cellular ligands results in functional consequences such as cell adhesion, shape change, and migration. that is crucial to cellular functions in several cell species. Thus, the development of these substrates for cell adhesion can provide new insights into cell biology as well as sophisticated methodology for controlling morphogenesis of cultured cells and tissues.This project consisted of three parts; the design of culture surface and characterization, understanding of cell attachment and morphology on D-glucose-displayed surface as the fundamental study, and its application for the cell functions. The first part covered the surface design and characterization. The second part which contained the last two themes deals with the elucidation of mechanism of morphological changes based on glucose-displayed surface; morphological changes of epithelial cells on glucose-displayed surface, morphological regulation of chondrocytes, and subsequently, chondrogenic expression on glucose-displayed surface. In the last part, we proposed that new function arising from culture surface such as targetability and adhesion to glucose-displayed surface could introduce a novel signal-transduction system to be applied for tissue construction.

已知锚定依赖细胞与底物的黏附调节多种细胞事件，如信号、基因表达和蛋白发生变化。特别是，整合素与细胞配体的结合会导致细胞黏附、形状改变和迁移等功能后果。这对几个细胞物种的细胞功能至关重要。因此，这些用于细胞黏附的底物的开发可以为细胞生物学提供新的见解，并为控制培养细胞和组织的形态发生提供复杂的方法学。本项目包括三个部分：培养表面的设计和表征，作为基础研究的细胞在D-葡萄糖显示表面的附着和形态的理解，以及它在细胞功能方面的应用。第一部分为表面设计与表征。第二部分包括后两个主题，涉及基于葡萄糖显示表面的形态变化机制的阐明，葡萄糖显示表面上皮细胞的形态变化，软骨细胞的形态调控，以及随后在葡萄糖显示表面的软骨细胞表达。在论文的最后部分，我们提出了培养表面产生的新功能，如靶向性和对葡萄糖展示表面的粘附性，将为组织构建提供一种新的信号转导系统。

项目成果

グルコース提示面による軟骨細胞の形態制御

葡萄糖呈递表面对软骨细胞形态的控制

• 发表时间: 2005
• 作者: Yuji Komizu;Yoko Matsumoto;Ryuichi Ueoka;紀ノ岡正博;Hideaki Nagami;Yiji Komizu;森永義樹
• 通讯作者: 森永義樹

Stimulated Migration of Human Epithelial Cells on Co-immobilized Surface of D-glucose and EGF

刺激人上皮细胞在 D-葡萄糖和 EGF 共固定表面上的迁移

• 发表时间: 2007
• 作者: Yoko Matsumoto;Yoshihiro Tanaka;Koichi Goto;Ryuichi Ueoka;Mee-Hae Kim
• 通讯作者: Mee-Hae Kim

D-グルコース提示面を用いた軟骨細胞培養における集塊形成機構の解明

使用 D-葡萄糖呈现表面阐明软骨细胞培养中的团聚机制

• 发表时间: 2007
• 作者: Hideaki Ichihara;Hideaki Nagami;Tetsuyuki Kiyokawa;Yoko Matsumoto;Ryuichi Ueoka;澤田佳子
• 通讯作者: 澤田佳子

D-グルコース提示面による細胞形態変化の機構解析と細胞集塊形成培養への応用

D-葡萄糖呈递表面引起的细胞形态变化的机理分析及其在细胞团聚培养中的应用

• 发表时间: 2008
• 作者: Mee-Hae Kim;Masahiro Kino-oka;Masaya Kawase;Kiyohito Yagi;Masahito Taya;Hideaki Ichihara;金 美海
• 通讯作者: 金 美海

Morphological Regulation of Chondrocytes on Glucose-Displayed Dendrimer Surface

葡萄糖显示树枝状聚合物表面软骨细胞的形态调控

• 发表时间: 2005
• 作者: Yuji Komizu;Yoko Matsumoto;Ryuichi Ueoka;紀ノ岡正博;Hideaki Nagami;Yiji Komizu;森永義樹;Hideaki Nagami;Masahiro Kino-oka
• 通讯作者: Masahiro Kino-oka