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Sth/dual and trarisuiptionalchange ofrice genome inducedby transposon, mPing

转座子 mPing 诱导的水稻基因组的双重和反转录变化

基本信息

批准号：
17380003
负责人：
TANISAKA Takatoshi
金额：
$ 10.73万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380003/
关键词：
rice transposon MITE genome structure gene regulation mPing ゲノム染色体構造染色体再編転移機構

项目摘要

MITEs play important roles in evolution by causing genome rearrangements and by altering the structure and regulation of individual genes. Non-autonomous element mPing is the first active MITE which is identified in rice (Oryza satires L.). Previous our researches showed that mPing is actively transposing in Gimbozu under natural conditions and preferentially inserted into single-copy regions. Transposition of mPing relay on transposases encoded by autonomous Ping and Pong. Rice cultivars have different copy number of Ping and Pong. In this study, we identified a novel transposon Pyong belong to Tc1/mariner super family through the analysis of copy number of Ping in Gimbozu. The blastn search identified two sequences sharing high homology with Pyong (>90%) on chromosome 1 and 12 of Nipponbare, respectively. This suggests the recent transposition of Pyong. Moreover, we carried out transposon display analysis using cDNA derived from Nipponbare, Gimbozu and a slender glume mutant IM294. As a result, 11 regions transcribed with mPing were identified. Among them, six and five regions were transcriptional and non-conding regions, respectively. Cultivars/strain used in this study showed mPing insertion polymorphism in these regions. Transcript level of mPing-inserted gene lowered at 3' region from mPing insertion site. These results indicate that mPing insertion alter not only genome structure but also the expression of adjacent regions.

MITE通过引起基因组重排和改变单个基因的结构和调控在进化中发挥重要作用。非自治元件mPing是在水稻中发现的第一个活性螨。我们以前的研究表明，mPing在自然条件下在金博祖中积极转座，并优先插入单拷贝区域。mPing的转座中继由自主Ping和Pong编码的转座酶。水稻品种具有不同的Ping和Pong拷贝数。本研究通过对Ping在Gimbozu中拷贝数的分析，鉴定出一个新的转座子Pyong，属于Tc 1/mariner超家族。Blasting搜索发现了两个与Pyong具有高度同源性（>90%）的序列，分别位于日本晴的1号和12号染色体上。这表明平壤最近的换位。此外，我们进行了转座子展示分析使用的cDNA来自日本晴，金宝津和细长颖突变体IM 294。其结果是，11个区域转录与mPing被确定。其中，6个区域为转录区，5个区域为非编码区。本研究中使用的品种/菌株在这些区域中显示mPing插入多态性。mPing插入基因的转录水平在mPing插入位点的3'区降低。这些结果表明mPing插入不仅改变基因组结构，还改变邻近区域的表达。