Molecular characterization and role of ecdysone membrane receptor

蜕皮激素膜受体的分子特征和作用

• 批准号: 17380035
• 负责人: SAKURAI Sho
• 金额: $ 10.81万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380035/
• 关键词: silk worm; Bombyx mori; programmed cell death; 20-hydroxyecdysone; sienal transduction; 膜受容体; 変態; 絹糸腺; カスパーゼ; カルシウム

We clarified developmental profiles of gene expression of early response genes to 20E in the anterior silk glands during the fifth instar up to the time of cell death execution two days after gut purge. Also, we showed the gene response to 20E in vitro using anterior silk glands of gut-purged larvae. The in vivo and in vitro results indicated that a heterodimeric EcR-B1 and USP-2 may be responsible for the cell death Results also indicated involvement of E74, E75, BHR3 and BR-C isoforms, but not Ftz-F1. We are not succeeded in gene cloning of the putative membrane ecdysone receptor yet. We examined pharmacologically the signaling pathway from mEcR to cellular responses, i.e. cell condensation, nuclear condensation, DNA fragmentation and nuclear fragmentation. Ca<2+> acts as the second messenger The mEcR is suggested to be a G-protein coupled receptor (GPCR) associated with Gαq, followed by a serial activation of phospholipase c-β, generation of inositol 3-phosphate (IP_3), and release of Ca<2+> from endoplasmic reticulum probably through IP3 receptor Then, Ca<2+> activates protein kinase C (PKC) and caspase 3-like protease. This signaling pathway culminates in nuclear fragmentation and nuclear fragmentation. Nuclear condensation is regulated by a different pathway involving calmodulin and calmodulin-dependent protein kinase II (CaMK-II). However, this pathway was not activated by Ca<2+>, and therefore it is unknown whether Gαq is involved in this pathway. In addition, inhibitors of calmodulin and CaMK-II affected the occurrence of nuclear and DNA fragmentations, indicating the caspase 3-like protease activation does not depend simply on the signaling pathway of GPCR/PLC-β/IP3/Ca<2+>/PKC.

我们澄清的早期反应基因的基因表达的发育概况20 E在前丝腺在第五龄的细胞死亡执行后两天肠道清洗的时间。此外，我们显示了基因反应20 E在体外使用前丝腺的肠道清洗幼虫。体内和体外结果表明，异源二聚体EcR-B1和USP-2可能是导致细胞死亡的原因。结果还表明，E74、E75、BHR 3和BR-C亚型参与，但Ftz-F1不参与。目前尚未成功地克隆出膜蜕皮激素受体的基因。我们研究了从mEcR到细胞反应的信号通路，即细胞凝聚，核凝聚，DNA片段化和核片段化。Ca ~（2+）是第二信使。mEcR可能是一个G蛋白偶联受体（GPCR），与Gαq结合，激活磷脂酶c-β，产生肌醇3-磷酸（IP_3），并可能通过IP_3受体释放Ca ~（2+），然后激活蛋白激酶C（PKC）和caspase 3样蛋白酶。该信号通路在核碎裂和核碎裂中达到高潮。核凝聚是由一个不同的途径，涉及钙调蛋白和钙调蛋白依赖性蛋白激酶II（CaMK-II）的调节。然而，该通路不被Ca<2+>激活，因此Gαq是否参与该通路尚不清楚。此外，钙调素和CaMK-Ⅱ的抑制剂可影响细胞核和DNA断裂的发生，表明caspase 3样蛋白酶的激活并不仅仅依赖于GPCR/PLC-β/IP 3/Ca<2+>/PKC信号通路。

项目成果

Nutritional status affects 20-hydroxyecdysone concentration and progression of oogenesis in Drosophila melanogaster.

• DOI: 10.1677/joe.1.06220
• 发表时间: 2005-10
• 期刊: The Journal of endocrinology
• 作者: J. Terashima;K. Takaki;S. Sakurai;M. Bownes

Spatial distribution of 20-hydroxyecdysone (20E)-responsive genes in the brain of silkworm, Bombyx mori.

家蚕 Bombyx mori 大脑中 20-羟基蜕皮激素 (20E) 反应基因的空间分布。

• 发表时间: 2007
• 作者: Roy;A.
• 通讯作者: A.

カイコ変態時に脳においてエクジソンにより誘導される遺伝子の網羅的解析-昆虫生理学的アプローチによる脳機能の解明.

蚕变态过程中大脑中蜕皮激素诱导基因的综合分析 - 使用昆虫生理学方法阐明大脑功能。

• 发表时间: 2005
• 作者: Sakiko;Shimizu;Monwar;Hossain;Yu;Kaneko;Sho;Sakurai;Hiroaki,Noda;Kazuei Mita;Masafumi;Iwami;松木正尋
• 通讯作者: 松木正尋

カイコガ幼虫脳におけるエクジソン応答遺伝子のマイクロアレイによる網羅的解析.

使用微阵列综合分析蚕幼虫大脑中的蜕皮激素反应基因。

• 发表时间: 2005
• 作者: Iga;M;清水咲子
• 通讯作者: 清水咲子

Identification, characterization, and developmental regulation of two storage proteins in the bamboo borer Omphisa fuscidentalis.

• DOI: 10.1016/j.jinsphys.2007.08.003
• 发表时间: 2008
• 期刊: Journal of insect physiology
• 影响因子: 2.2
• 作者: Jatuporn Tungjitwitayakul;T. Singtripop;Anchalee Nettagul;Y. Oda;Nujira Tatun;Takayuki Sekimoto;S. Sakurai