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Research and Development of Cellulose Degradation Method with Ammonia

氨降解纤维素方法的研究进展

基本信息

批准号：
17380158
负责人：
TAKAHASHI Junichi
金额：
$ 9.89万
依托单位：
Obihiro University of Agriculture and Veterinary Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380158/
关键词：
bone-char meat bone meal BSE prion resource recycling 家畜屠体資源循環異常プリオン

项目摘要

The objective of present study is to establish and design a model of resource recycling carbonization for a prompt, low-input and safe agricultural materials made from meat bone meal, carcass and animal waste etc. exhausted from slaughter house.The BSE infected hamster brain was carbonized under a different temperature condition, the manufactured products were vaccinated to the mouse brain, and a safe manufacturing condition was examined from the results of infectious proof examination.1. Study on the manufacturing carbonized productsIn the carbonization of meat bone meal or hamster brain, observation of carbonization and the interfacestute, distribution of surface elements and functional groups etc. were examined by Raman spectrometry, FTIR, and SEM・EDX, etc. for carbonized products. In consequence, if about one hour was surely heat-treated in 500℃-600℃, the protein structure was completely destroyed and then thoroughly carbonized products will be obtained.2. Evaluation of BSE infecti … More onThe BSE infected hamster brain has been carbonized within the lowest range of temperature and rage for the carbonization with a carbonization experiment device in P2 laboratory of Prion Disease Research Center, National Institute of Animal Health (Ibaraki, Japan). In addition, in order to reconfirm the animal charcoal manufacturing guideline, the BSE infected hamster brain was made an ash under the same temperature condition as the guideline. These carbonized products were vaccinated to the normal transgenic mouse brain in the P3 laboratory, and an infectious examination was executed. The vaccination of unprocessed the infected brain into mice brain was confirmed PrP^<res> in all by the western blotting and died by the BSE infection within 45 days. PrP^<res> was not detected as a result of the inspection in both carbonization processing (400, 600, 800℃) and processing (600℃) of making to the ash after vaccinations passed for 525 days, and the infection of BSE was not admitted.3. Construction of total systemA safe carbonizing total system to prevent the exhaust of organic stuff such as amines in the processing water to outside with membrane filtration was developed. The present manufacturing system of bone char is the most effective safe way to treat the meat bone meal and produces the additional values as absorbents and soil improvement materials. Less

本研究的目的是建立和设计一种资源循环碳化模型，以屠宰场排出的肉骨粉、屠体和动物粪便等为原料，快速、低投入、安全地生产农产品。将感染疯牛病的仓鼠脑在不同温度条件下碳化，将生产出来的产品接种到小鼠脑内，通过感染性检验的结果来检验安全的生产条件。利用拉曼光谱、傅里叶变换红外光谱、扫描电子显微镜等对炭化产物的表面元素和官能团分布等进行了观察和研究。因此，如果一定要在500℃-600℃的温度下加热约1小时，蛋白质的结构将被完全破坏，从而获得完全碳化的产物。疯牛病感染性…的评价此外，在日本茨城国家动物卫生研究所Prion疾病研究中心P2实验室的碳化实验装置中，感染BSE的仓鼠脑已在碳化的最低温度和范围内碳化。此外，为了再次确认动物炭制作指南，在与指南相同的温度条件下，将感染疯牛病的仓鼠脑制成灰烬。将这些碳化产物接种到P3实验室的正常转基因小鼠脑内，并进行感染性检查。经免疫印迹证实，未处理的感染脑接种小鼠脑内，45天内即死于BSE。接种525天后，炭化工序(400、600、800℃)和灰化工序(600℃)检查均未检出PRP，未检出疯牛病。建立全程安全碳化全程系统，防止处理水中的胺等有机物通过膜过滤向外排放。目前的骨炭生产体系是处理肉骨粉的最有效、最安全的方法，并产生了作为吸附剂和土壤改良材料的附加值。较少