Evaluation of periodontal pathogenic microorganisms and periodontitis using rapid DNA probe method.

The aim of The present study was to develop a rapid DNA probe method for The diagnosis ofperiodontitis that can be used in the dental clinics. the method minimizes the use of water bath forordinary hybrydization and washing in order to shorten the total time. Samples与hybridized withboiled DNA probeand then washed thoroughly with 0.2×SSC/0.1 SDS at 75℃. Detection过程could be completed within2 hours. Detection level was more than 10 bacterial cells/sample.Subgingival plaquesamples taken from 468 sites in 55 patients with periodontitis before the periodontal initialtherapy After the periodontal therapy of scaling and rootplaning58 sites in 9 patients with periodontitis were bacteriologically and cliically evaluated. When theDNA probe method was compared with the文化方法，the acuracy was 88% for P. gingivalis，67% for A. actinomycetemcomitans. A significant association was found between the detection of P.gingivalis and PD, BOP (x 2 test，p<0.01). A significant association was also shown between the detection of A. actinomycetemcomitansPD in patients whose ages were 35 or older (x 2 test，p<0.01). The关系between A. actinomycetemcomitans and BOP was not significant. TheA. actinomycetemcomitans was highest in teenagers. At shallow periodontal pocketsites (PD<4mm) in teenagers, no P. gingivalis was found，22% of the sites harbored A. actinomycetemcomitans. After the therapy，the proportions of P. gingivalis decreased significantly only in the clinically resolved sites(% 2test, p<0.01). However，A. actinomycetemcomitans seemed to persist after the therapy.The rapid DNA probe method appearspromising as an efficient tool for rapid diagnosis, selection of treatment modalities，and microbiological evaluation of the treatment结果。

The aim of the present study was to develop a rapid DNA probe method for the diagnosis of periodontitis that can be used in the dental clinics. The method minimizes the use of water bath for ordinary hybrydization and washing in order to shorten the total time. Samples were hybridized with boiled DNA probe, and then washed thoroughly with 0.2×SSC/0.1% SDS at 75℃. Detection process could be completed within 2 hours. Detection level was more than 10ィイD14ィエD1 bacterial cells/sample.Subgingival plaque samples were taken from 468 sites in 55 patients with periodontitis before the periodontal initial therapy. After the periodontal therapy of scaling and rootplaning, 58 sites in 9 patients with periodontitis were bacteriologically and cliically evaluated. When the DNA probe method was compared with the culture method, the acuracy was 88% for P. gingivalis, 67% for A. actinomycetemcomitans. A significant association was found between the detection of P. gingivalis and PD, BOP (x 2 test, p<0.01). A significant association was also shown between the detection of A. actinomycetemcomitans and PD in patients whose ages were 35 or older (x 2 test, p<0.01). The relationship between A. actinomycetemcomitans and BOP was not significant. The detection rate of A. actinomycetemcomitans was highest in teenagers. At shallow periodontal pocket sites (PD<4mm) in teenagers, no P. gingivalis was found, while 22% of the sites harbored A. actinomycetemcomitans. After the therapy, the proportions of P. gingivalis decreased significantly only in the clinically resolved sites(% 2 test, p<0.01). However, A. actinomycetemcomitans seemed to persist after the therapy.The rapid DNA probe method appears promising as an efficient tool for rapid diagnosis, selection of treatment modalities, and microbiological evaluation of the treatment outcome.