Evaluation of periodontal pathogenic microorganisms and periodontitis using rapid DNA probe method.

当前研究的目标是为周期性诊断开发一种快速DNA探针方法，该方法可以用于牙科诊所。该方法最小化了水浴用于常规杂交和洗涤的方法，以缩短总时间。样本用Boiled DNA探针杂交，然后用0.2 × SSC/0.1%SDS洗涤，在75 ℃。探测过程可以在2小时内完成。检测级别高于10个D14个D14个D1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1B1在进行了扩展和根规划的周期性治疗后，有9个病人的周期性肿瘤是细菌学和实时评估的58个站点。当DNA探测方法与文化方法相比较时，纯度为88%，A为67%。actinomycetemcomitans。在P.Gingivalis和PD的检测之间发现了一个重要的关联（x2测试，p <0.01）。一个有意义的关联在A的检测之间也是显而易见的。患者中的活性霉素comitans和PD患者中的年龄为35岁或以上（x2测试，p <0.01）。A.之间的关系。Acinomycetemcomitans和BOP并不重要。A的检测率。Actinomycetemcomitans是青少年中最好的。在青少年中发现的浅周期口袋站点（PD <4毫米），但发现了P.Gingivalis，同时该站点占22%。actinomycetemcomitans。在治疗后，P.Gingivalis的情况明显地只在临床上解决的站点（%2测试，p <0.01）。However，A.活性霉素comitans似乎要在治疗后坚持下去。快速DNA探针方法似乎被认为是快速诊断的有效工具、治疗模式的选择和治疗结果的微生物学评估。

The aim of the present study was to develop a rapid DNA probe method for the diagnosis of periodontitis that can be used in the dental clinics. The method minimizes the use of water bath for ordinary hybrydization and washing in order to shorten the total time. Samples were hybridized with boiled DNA probe, and then washed thoroughly with 0.2×SSC/0.1% SDS at 75℃. Detection process could be completed within 2 hours. Detection level was more than 10ィイD14ィエD1 bacterial cells/sample.Subgingival plaque samples were taken from 468 sites in 55 patients with periodontitis before the periodontal initial therapy. After the periodontal therapy of scaling and rootplaning, 58 sites in 9 patients with periodontitis were bacteriologically and cliically evaluated. When the DNA probe method was compared with the culture method, the acuracy was 88% for P. gingivalis, 67% for A. actinomycetemcomitans. A significant association was found between the detection of P. gingivalis and PD, BOP (x 2 test, p<0.01). A significant association was also shown between the detection of A. actinomycetemcomitans and PD in patients whose ages were 35 or older (x 2 test, p<0.01). The relationship between A. actinomycetemcomitans and BOP was not significant. The detection rate of A. actinomycetemcomitans was highest in teenagers. At shallow periodontal pocket sites (PD<4mm) in teenagers, no P. gingivalis was found, while 22% of the sites harbored A. actinomycetemcomitans. After the therapy, the proportions of P. gingivalis decreased significantly only in the clinically resolved sites(% 2 test, p<0.01). However, A. actinomycetemcomitans seemed to persist after the therapy.The rapid DNA probe method appears promising as an efficient tool for rapid diagnosis, selection of treatment modalities, and microbiological evaluation of the treatment outcome.