Inventory of natural manipulators to mitigate greenhouse gases emission from ruminants

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To develop a technology for the mitigation and utilization of greenhouse gases which are methane and nitrous oxide attributed to animal agriculture, in vitro rumen fermentation trials using in vitro continuous incubation system and analyses of rumen micro flora ecosystem including methanogens using PCR method were conducted. In consequence, some prebiotics and probiotics were nominated for the inventory of natural manipulators to mitigate greenhouse gases from ruminant animals.