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Development of trace analysis of perfluorochemicals in human biological samples and assessment of exposure

人类生物样品中全氟化合物痕量分析和暴露评估的发展

基本信息

批准号：
17390036
负责人：
NAKAZAWA Hiroyuki
金额：
$ 10.25万
依托单位：
Hoshi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

For the development of the trace analysis of perfluorochemicals(PFCs) in biological samples and evaluation of human exposure were investigated by the 2005〜2007 fiscal year.The 2005 fiscal year : For PFCs measurement of the human plasma, the analytical method using the column switching liquid chromatography-tandem mass spectrometry(LC-MS/MS) has been studied, and the method was applied to the analysis of PFCs in maternal blood and umbilical cord blood. The method is highly sensitive and selective for the monitoring of PFCs in human plasma. The proportional relation of concentration of PFCs between maternal blood and umbilical cord blood was obtained. The transport of PFCs from maternal blood into fetal blood was speculated.The 2006 fiscal year: In order to elucidate the exposure source of PFCs, which have been detected in human blood with high frequency, the analytical method by use of supercritical fluid extraction (SFE) and LC-MS/MS was investigated for measurement of PFCs in house dust samples. The developed analytical method which used SFE for the pretreatment was successfully applied to analysis of house dusts with high sensitivity and repeatability. In comparison with other environmental samples, the concentration of PFCs in house dust is remarkably higher and considered as one of the major human exposure source.The 2007 fiscal year : Based on the transport of PFCs to the fetus through the umbilical cord blood from the mother the analytical method of PFCs in mother's milk with highly sensitivity was carried out with solid phase extraction and LC-MS/MS. In results, it was indicated that the mother's milk was one of the transport pathway of PFCs to the infant from the mother.

为了开发生物样品中全氟化学品（PFCs）的痕量分析和人类接触评估，在2005 - 2007财政年度进行了调查。对于人血浆中PFCs的测量，使用柱切换液相色谱-串联质谱的分析方法建立了液相色谱-串联质谱（LC-MS/MS）联用技术，并应用于母血和脐带血中PFCs的分析。该方法具有较高的灵敏度和选择性，适用于人血浆中全氟碳化物的监测。得出了母血和脐血中PFCs浓度的比例关系。2006财政年度：为了阐明在人体血液中检出频率较高的PFCs的暴露来源，采用超临界流体萃取（SFE）和LC-MS/MS分析方法对室内灰尘样品中PFCs进行了测定。该方法灵敏度高，重现性好，已成功地应用于室内灰尘的分析。与其他环境样品相比，室内灰尘中的PFCs浓度明显较高，被认为是人类的主要接触源之一。基于PFCs可通过母亲脐带血转运至胎儿体内的特点，采用固相萃取-液相色谱-串联质谱法对母乳中PFCs进行了高灵敏度分析。说明母乳是PFCs从母体向婴儿转运的途径之一。