Development of trace analysis of perfluorochemicals in human biological samples and assessment of exposure

为开展生物样品中全氟化合物(PFC)痕量分析和人体暴露评价工作，于2005~2007财年开展了生物样品中全氟化合物的痕量分析研究。2005财年：针对人体血浆中PFC的测定，研究了柱切换液相色谱串联质谱仪(LC-MS/MS)的分析方法，并将该方法应用于母血和脐血中PFC的分析。该方法具有较高的灵敏度和选择性，可用于监测人血浆中的全氟碳化合物。得到了母血和脐血中全氟碳酸盐浓度的比例关系。2006财年：为了弄清人体血液中检测到的高频全氟碳化物的暴露来源，采用超临界流体萃取和LC-MS/MS联用技术对室内粉尘中全氟碳化物的分析方法进行了研究。所建立的超临界流体萃取前处理分析方法成功地应用于室内粉尘的分析，具有较高的灵敏度和重现性。与其他环境样品相比，室内粉尘中全氟碳化物的浓度明显偏高，被认为是主要的人体接触来源之一。2007财年：根据全氟碳化物从母亲经脐带血转运到胎儿的情况，利用固相萃取和LC-MS/MS对母乳中全氟碳化物进行了高灵敏度的分析。结果表明，母乳是全氟碳化合物从母亲到婴儿的运输途径之一。

For the development of the trace analysis of perfluorochemicals(PFCs) in biological samples and evaluation of human exposure were investigated by the 2005〜2007 fiscal year.The 2005 fiscal year : For PFCs measurement of the human plasma, the analytical method using the column switching liquid chromatography-tandem mass spectrometry(LC-MS/MS) has been studied, and the method was applied to the analysis of PFCs in maternal blood and umbilical cord blood. The method is highly sensitive and selective for the monitoring of PFCs in human plasma. The proportional relation of concentration of PFCs between maternal blood and umbilical cord blood was obtained. The transport of PFCs from maternal blood into fetal blood was speculated.The 2006 fiscal year: In order to elucidate the exposure source of PFCs, which have been detected in human blood with high frequency, the analytical method by use of supercritical fluid extraction (SFE) and LC-MS/MS was investigated for measurement of PFCs in house dust samples. The developed analytical method which used SFE for the pretreatment was successfully applied to analysis of house dusts with high sensitivity and repeatability. In comparison with other environmental samples, the concentration of PFCs in house dust is remarkably higher and considered as one of the major human exposure source.The 2007 fiscal year : Based on the transport of PFCs to the fetus through the umbilical cord blood from the mother the analytical method of PFCs in mother's milk with highly sensitivity was carried out with solid phase extraction and LC-MS/MS. In results, it was indicated that the mother's milk was one of the transport pathway of PFCs to the infant from the mother.