Detection of perfuorooctanoic acid (PFOA) and perfluorooctane sulfonate(PFOS) in liquid samples.

检测液体样品中的全氟辛酸 (PFOA) 和全氟辛烷磺酸 (PFOS)。

• 批准号: 10010066
• 负责人: Sanjay V Patel
• 金额: $ 15万
• 依托单位: SEACOAST SCIENCE, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-23 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10010066
• 关键词: Acids; Adverse effects; Alkanesulfonates; Animals; Area; Binding; Biological; Blood; Cholesterol; Complex; Crosslinker; Crystallization; Data; Data Analyses; Data Analytics; Deposition; Detection; Devices; Environment; Environmental Monitoring; Formulation; Freedom; Future; Government Agencies; Human; Industrialization; Ionic Strengths; Laboratories; Lead; Liquid substance; Mass Fragmentography; Measures; Methods; Molecular; Nature; Outcome; Pathway interactions; Peer Review; Performance; Phase; Poly-fluoroalkyl substances; Polymers; Population; Pre-Eclampsia; Production; Property; Publications; Quartz; Reporting; Research; Resistance; Risk; Sales; Saliva; Sampling; Science; Serum; Site; Source; Sperm Count Procedure; Structure; Surface; Surface Properties; System; Technology; Teflon; Testing; Time; Universities; Urine; Water; Work; aqueous; base; bioaccumulation; cost; crosslink; design; epidemiology study; experimental study; field study; imprint; manufacturing facility; minimally invasive; monomer; nanocomposite; nanoparticle; novel; particle; perfluorooctane sulfonate; perfluorooctanoic acid; professor; prostate cancer risk; prototype; real time monitoring; response; screening; sensor; tool; water treatment

Perfluoroalkyl substances (PFAS) such as perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate) are toxic and persistent compounds resulting from the production and use of fluoropolymers such as Gortex® and Teflon ®. The structure of PFAS preclude environmental degradation and can lead to bioaccumulation in animals and humans. Reported adverse effects of these compounds in humans include: increase in serum cholesterol; elevated risk of prostate cancer; decreased sperm count; and increased risk of preeclampsia. This proposal describes the synthesis of fluorinated molecularly imprinted polymers (FMIPs) as materials that not only specifically bind the target analytes but also by the design of the material surface limit non-specific binding. The FMIPs will be composed of a novel fluorinated crosslinking monomer with 2 different functionalized co-monomers. The proposed device will use these materials deposited on a proprietary sensor platform developed at Seacoast Science. During the Phase I work, the concept will be validated against a representative PFOA and PFOS in in water. To accomplish the Phase I proof of concept work, the following tasks are proposed: synthesis and characterization of novel fluorinated crosslinking monomer, synthesis and and characterization of FMIP nanoparticles; initial screening/down selection of MIP nanoparticles using a gravimetric sensor platform; and coating and testing optimum MIP nanoparticles on the proprietary sensor platform. This tool will allow the real-time monitoring of PFOA/PFOS in fluids. Early adopters of the technology will be government agencies tasked with environmental monitoring. After this initial use, field testing, and production optimization, other more risk averse agencies such as water districts may be convinced to purchase the technology for point detection. The final market is the industrial sources of other perfluoroalkyl substances (PFAS). The modular nature of the FMIP synthesis allows for a facile change in the imprinting analyte: the use of systems and methods developed during this project will allow for rapid optimization of the sensor to a novel PFAS (i. e. F53-B, ADONA, FOSA, E1, 5:3 FTSA). Finally, once the sensor has been on the market for a sufficient time, it may be adapted for the detection of PFAS in more complex matrices like biological fluids. This tool will allow wide spread epidemiological studies looking at PFA exposure levels for large populations.

全氟烷基物质（PFAS），如全氟辛酸（PFOA）和全氟辛烷磺酸（PFAS）， 生产和使用含氟聚合物（如Gortex®）产生的有毒和持久性化合物， Teflon®. PFAS的结构阻碍了环境降解，并可导致生物累积， 动物和人类。这些化合物对人体的不良反应包括： 胆固醇;前列腺癌风险升高;精子数量减少;先兆子痫风险增加。这 该提案描述了氟化分子印迹聚合物（FMIP）的合成， 不仅特异性结合目标分析物，而且受材料表面设计的限制， 约束力FMIP将由一种新型氟化交联单体与2种不同的 官能化共聚单体。拟议的设备将使用这些材料沉积在一个专有的传感器 在Seacoast Science开发的平台。在第一阶段工作期间，将根据 水中的代表性PFOA和PFOS。为了完成第一阶段的概念验证工作， 提出的任务是：新型含氟交联单体的合成与表征， FMIP纳米颗粒的初步筛选和表征; 重量传感器平台;以及在专有传感器上涂覆和测试最佳MIP纳米颗粒 平台该工具将允许实时监测液体中的全氟辛酸/全氟辛烷磺酸。的早期采用者 技术将是负责环境监测的政府机构。在首次使用后， 测试和生产优化，其他更规避风险的机构，如水务区，可能会被说服 购买点检测技术。最后的市场是其他的工业来源 全氟烷基物质（PFAS）。FMIP合成的模块化性质允许在 印迹分析物：使用本项目期间开发的系统和方法， 将传感器优化为新型PFAS（i. e. F53-B，ADONA，FOSA，E1，5：3FTSA）。最后，一旦传感器 已经在市场上有足够的时间，它可以适用于检测PFAS在更复杂的 像生物液体一样的基质。这一工具将允许对PFA进行广泛的流行病学研究 大规模人群的暴露水平。