Study of human exposure assessment of chemical materials in daily life and their endocrine disrupting activity.

日常生活中化学物质的人体暴露评估及其内分泌干扰活性研究。

基本信息

  • 批准号:
    12490030
  • 负责人:
  • 金额:
    $ 6.27万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

Endocrine disrupting chemicals (EDCs) consist of synthetic and naturally occurring chemicals that affect the balance of hormonal functions in humans and animals. Some pesticides and industrial chemicals can affect animal physiology by mimicking the effects of endogenous hormones. The potential risks to human health and reproduction from the EDCs have not been well established. This is due in part to the absence of the accurate analytical methods to quantify EDCs in human biological samples and the in vitro assay of estrogenic activity for risk assessment of the EDCs to human. In this study we establish an accurate, sensitive and selective analytical method for the quantification of EDCs such as bisphenol A (BPA), hisphenol A diglycidyl ether (BADGE), alkylphenols, phthalate esters and isoflavones in human biological samples. In addition, the estrogenic activity of many synthetic chemicals was investigated by using cell proliferation assay and receptor competitor binding assay.We have a … More chieved the study of BPA migration in canned drink and other food-stuff and the investigation of the possibility of contamination of honey. Because of these studies of BPA and their potential role in increasing risk for reproductive dysfunction and cancer, the need for an assessment of human exposure is urgent. Therefore, we developed the methods for measurement of BPA and BADGE metabolites in human biological samples by HPLC, LC/MS and GC/MS, and the assays for estimation of estrogenic activity of these chemicals by cell proliferation and receptor binding effects. On the other hands, some estrogenic chemicals were reported to be leached out from such PVC products such as food wrapping films, medical devices. In this reason, we employed column-switching LC/MS coupled with on-line extraction for the sensitive, selective and accurate determination of EDCs in respet with the PVC products. This method was successfully used to determinate EDCs in human biological samples from healthy humans, and the main, source of human exposure was clarify. Less
内分泌干扰物(EDCs)由人工合成和自然产生的影响人类和动物荷尔蒙功能平衡的化学物质组成。一些杀虫剂和工业化学品可以通过模仿内源激素的影响来影响动物的生理。EDC对人类健康和生殖的潜在风险尚未得到很好的确定。这在一定程度上是由于缺乏准确的分析方法来量化人体生物样本中的内分泌细胞,以及缺乏体外测定雌激素活性以评估内分泌细胞对人类的风险。在这项研究中,我们建立了一种准确、灵敏和选择性的分析方法,用于人体生物样品中双酚A(BPA)、组酚A二缩水甘油醚(BAGE)、烷基酚、邻苯二甲酸酯和异黄酮类EDCs的定量测定。此外,通过细胞增殖试验和受体竞争结合试验研究了许多合成化合物的雌激素活性。我们有一个…进一步开展了罐装饮料等食品中双酚A迁移的研究和蜂蜜污染的可能性调查。由于对双酚A的这些研究及其在增加生殖功能障碍和癌症风险方面的潜在作用,迫切需要对人类暴露进行评估。为此,我们建立了人体生物样品中BPA和BAGE代谢物的高效液相色谱、LC/MS和GC/MS测定方法,以及从细胞增殖和受体结合效应来评价这些化学物质的雌激素活性的方法。另一方面,据报道,一些雌激素化学物质被从食品包装膜、医疗器械等聚氯乙烯产品中浸出。为此,我们采用柱切换LC/MS与在线萃取相结合的方法,灵敏、选择性、准确地测定了聚氯乙烯产品中的内源性化合物。该方法成功地用于健康人体生物样品中内毒素的测定,明确了人体暴露的主要来源。较少

项目成果

期刊论文数量(136)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
INOUE, K., KAWAGUCHI M., OKADA, F., YOSHIMURA, Y. AND NAKAZAWA, H.: "Column-switching high-performance liquid chromatography electrospray mass spectrometry coupled with on-line of extraction for the determination of mono- and di-(2-ethylhexyl) phthalate i
INOUE, K.、KAWAGUCHI M.、OKADA, F.、YOSHIMURA, Y. 和 NAKAZAWA, H.:“柱切换高效液相色谱电喷雾质谱法与在线萃取相结合,用于测定单和
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INOUE, K, KATO, K., YOSHIMURA, Y., MAKINO, T. AND NAKAZAWA, H.: "Determination of bisphenol A in human serum by high-performance liquid chromatography with multi-electrode electrochemical detection."J. Chromatogr. B. 749. 17-23 (2000)
INOUE, K、KATO, K.、YOSHIMURA, Y.、MAKINO, T. 和 Nakazawa, H.:“采用多电极电化学检测的高效液相色谱法测定人血清中的双酚 A”。
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Inoue, K., Higuchi, T., Okada, F., Iguchi, H., Yoshimura, Y., Sato, A., Nakazawa, H.: "The validation of column-switching LC/MS as a high-throughput approach for direct analysis of di(2-ethylhexyl) phthalate released from PVC medical devices in intravenou
Inoue, K.、Higuchi, T.、Okada, F.、Iguchi, H.、Yoshimura, Y.、Sato, A.、Nakazawa, H.:“柱切换 LC/MS 作为高通量分析方法的验证
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    0
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Inoue, K., Murayama, S., Yoshimura, Y., Nakazawa, H.: "Contamination of xenoestrogens bisphenol A and F in honey : Safety assessment and analytical method of these compounds in honey"J. Food Compos. Anal.. (In press).
Inoue, K.、Murayama, S.、Yoshimura, Y.、Nakazawa, H.:“蜂蜜中异雌激素双酚 A 和 F 的污染:蜂蜜中这些化合物的安全性评估和分析方法”J。
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Nakazawa, H., Yamaguchi, A., Inoue, K., Yamazaki, T., Kato, K., Yoshimura, Y., Makino, T.: "In vitro assay of hydrolysis and chlorahydoroxy derivatives of bisphenol A diglycidyl ether for estrogenic activity"Food Chem.Toxicol.. 40. 1827-1832 (2002)
Nakazawa, H.、Yamaguchi, A.、Inoue, K.、Yamazaki, T.、Kato, K.、Yoshimura, Y.、Makino, T.:“双酚 A 二缩水甘油醚的水解和氯羟基衍生物的体外测定
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NAKAZAWA Hiroyuki其他文献

NAKAZAWA Hiroyuki的其他文献

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{{ truncateString('NAKAZAWA Hiroyuki', 18)}}的其他基金

Acupuncture points stimulation for stress management
穴位刺激用于压力管理
  • 批准号:
    15K01669
  • 财政年份:
    2015
  • 资助金额:
    $ 6.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of trace analysis of perfluorochemicals in human biological samples and assessment of exposure
人类生物样品中全氟化合物痕量分析和暴露评估的发展
  • 批准号:
    17390036
  • 财政年份:
    2005
  • 资助金额:
    $ 6.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Estimation of free radical formation and deletion of life relation substances
自由基形成和生命关系物质缺失的估计
  • 批准号:
    09672200
  • 财政年份:
    1997
  • 资助金额:
    $ 6.27万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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    10414318
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EAGER: Synthetic Chemical-Based Information Processing
EAGER:基于合成化学的信息处理
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    10662258
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Synthetic Chemical Biology Core
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    10270503
  • 财政年份:
    2016
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Synthetic Chemical Biology Core
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  • 批准号:
    10460249
  • 财政年份:
    2016
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Synthetic Chemical Biology
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