Identification and pharmacome analysis of receptor phenotypes originated from a single gene
源自单个基因的受体表型的鉴定和药组分析
基本信息
- 批准号:17390064
- 负责人:
- 金额:$ 9.95万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Every protein such as a receptor or enzyme is biosynthesized according to one corresponding gene. However, after expression, proteins may be frequently modified by many factors innative cells and tissues, so that these proteins no longer exhibit a single property. We have proposed t hat all of such proteins occurring in native tissues are designated as the `pharmacome'. In this study, we performed pharmacome analysis of α1-adrenoceptor (AR). At first, we found a unique a1-AR mediating adrenergic contraction in lower urinary tract and named as a1 L-AR because of its low affinity for prazosin. The a1 L-AR was identified by binding approach with intact tissue segments but not with membrane preparations. Thus, aft-AR converted to a1A-AR up on homogenization, suggesting a possible relationship between the both a1-ARs. Then we examined the effects of knockout (KO) of a1 -ARs. In wild-type, a1B-KO and a1D-KO mice, a1 L-AR was identified in functional and binding studies. However, in a1 A-K0 mice, not only a1A-AR but also a1L-AR was abolished. From these results it is concluded that both a1A-and a 1L-ARs are derived from a1A-AR gene, indicating that different phenotypes are expressed from a single gene.
每一种蛋白质,如受体或酶,都是根据一个相应的基因进行生物合成的。然而,在表达后,蛋白质可能经常被天然细胞和组织中的许多因素修饰,使得这些蛋白质不再表现出单一的性质。我们已经提出,天然组织中存在的所有这些蛋白质都被称为“药物组”。本研究对α1-肾上腺素能受体(AR)进行了药理学分析。首先,我们在下尿路发现了一种独特的α 1-AR介导肾上腺素能收缩,因其与哌唑嗪的亲和力较低而命名为α 1 L-AR。通过与完整组织片段结合的方法鉴定α 1 L-AR,但不与膜制备物结合。因此,aft-AR在均质化时转化为a1 A-AR,表明两种a1-AR之间可能存在关系。然后,我们检查了α 1-AR基因敲除(KO)的影响。在野生型、a1 B-KO和a1 D-KO小鼠中,在功能和结合研究中鉴定了a1 L-AR。然而,在a1 A-K 0小鼠中,不仅a1 A-AR而且a1 L-AR被消除。从这些结果可以得出结论,α 1A-AR和α 1 L-AR都来源于α 1A-AR基因,表明不同的表型由单个基因表达。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Coupled K+-water flux through the HERG potassium channel measured by an osmotic pulse method
- DOI:10.1085/jgp.200509377
- 发表时间:2005-11-01
- 期刊:
- 影响因子:3.8
- 作者:Ando, H;Kuno, M;Oiki, S
- 通讯作者:Oiki, S
Comparison of the binding affinity of some newly synthesized phenylethanolamine and phenoxypropanolamine compounds at recombinant human β-and a1-adorenoceptor subtypes
一些新合成的苯乙醇胺和苯氧基丙醇胺化合物对重组人β-和α1-肾上腺素受体亚型的结合亲和力比较
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Ahmed;M.;Muramatsu;I.;et. al.
- 通讯作者:et. al.
Alpha-1 adrenoceptors: function, disease and aging,
Alpha-1 肾上腺素受体:功能、疾病和衰老,
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Muramatsu;I;Ikunobu Muramatsu
- 通讯作者:Ikunobu Muramatsu
α1アドレナリン受容体サブタイプの分類と目における分布・最近の話題
α1-肾上腺素能受体亚型的分类和在眼中的分布/近期主题
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Muramatsu;I;Ikunobu Muramatsu;Muramatsu;村松 郁延;Muramatsu;村松 郁延;Muramatsu;Muramatsu;村松 郁延;村松 郁延;Muramatsu;村松 郁延;Muramatsu;村松 郁延
- 通讯作者:村松 郁延
Pharmacome analysis of alpha-1 adrenoceptors in eye
眼中α-1肾上腺素受体的药理学分析
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Muramatsu;I;Ikunobu Muramatsu;Muramatsu;村松 郁延;Muramatsu;村松 郁延;Muramatsu;Muramatsu;村松 郁延;村松 郁延;Muramatsu;村松 郁延;Muramatsu;村松 郁延;Muramatsu;Muramatsu
- 通讯作者:Muramatsu
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MURAMATSU Ikunobu其他文献
MURAMATSU Ikunobu的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MURAMATSU Ikunobu', 18)}}的其他基金
Intracellular M1-muscarinic acetylcholine receptors in central nervous system
中枢神经系统细胞内 M1-毒蕈碱乙酰胆碱受体
- 批准号:
15K08250 - 财政年份:2015
- 资助金额:
$ 9.95万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Receptor-operated Ca influx and transmitter release
受体操作的 Ca 流入和递质释放
- 批准号:
20390068 - 财政年份:2008
- 资助金额:
$ 9.95万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular cloning and functional studies of alpha_1 adrenoceptors
α_1肾上腺素受体的分子克隆和功能研究
- 批准号:
09470023 - 财政年份:1997
- 资助金额:
$ 9.95万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study on sympathetic purinergic transmission in blood vessels
血管内交感嘌呤能传递的研究
- 批准号:
61570097 - 财政年份:1986
- 资助金额:
$ 9.95万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)