Induction of antigen specific peripheral tolerance by targeting Langerhans cells

通过靶向朗格汉斯细胞诱导抗原特异性外周耐受

• 批准号: 17390312
• 负责人: MORITA Akimichi
• 金额: $ 10.5万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390312/
• 关键词: ULTRAVIOLET; IMMUNE SUPPRESSION; EGURATORY T CELLS; Narrow-band UVB; 制御性T細胞; ランゲルハンス細胞; 樹状細胞; 免疫寛容; アレルギー疾患; 皮膚; 遅延型過敏反応; 自己免疫疾患

Phototherapy generally induces a relatively long remission period in patients with psoriasis, which cannot be explained simply by UV-induced apoptosis. Therefore, the role of regulatory T cells should also be considered. We recently reported that narrow-band UVB suppresses delayed-type hypersensitivity (DTH) and contact hypersensitivity by inducing regulatory T cell production. The UV wavelength that induced regulatory T cells to DTH, however, was not known. We irradiated a mouse DTH model using a monochromator to produce several UVB wavelengths (290 nm, 300 nm, 310 nm, and 320 nm). All wavelengths tested significantly suppressed DTH, with 300 nm inducing maximal suppression. Foxp3 expression in lymph node cells from tolerant mice was analyzed by real time polymerase chain reaction (mRNA) and FACS analysis (protein). Foxp3 induction was increased 2-fold at 300 nm compared with controls. Similarly, interleukin (IL)-10 induction was increased 10-fold at 300 nm. Conversely, IL-17, IL-23, and IL-12 were suppressed by 40%, 25%, and 30%, respectively, at 300 nm. These data indicate that 300-nm UV light induces Foxp3-expressing regulatory T cells and suppresses Th-17 cells. Besides inducing Foxp3-expressing regulatory T cells, the suppression of Th-17 cells might be important for the UV light-induced immune suppression. The use of a specific wavelength within the UVB range is a new strategy for the induction of antigen-specific peripheral tolerance and provides a new potential treatment for allergic dermatologic diseases.

光疗通常诱导银屑病患者相对较长的缓解期，这不能简单地用UV诱导的细胞凋亡来解释。因此，还应考虑调节性T细胞的作用。我们最近报道了窄谱UVB通过诱导调节性T细胞的产生来抑制迟发型超敏反应（DTH）和接触性超敏反应。然而，诱导调节性T细胞DTH的紫外线波长尚不清楚。我们使用单色仪照射小鼠DTH模型以产生几种UVB波长（290 nm、300 nm、310 nm和320 nm）。所有测试的波长均显著抑制DTH，其中300 nm诱导最大抑制。通过真实的时间聚合酶链反应（mRNA）和FACS分析（蛋白质）分析来自耐受小鼠的淋巴结细胞中Foxp 3的表达。与对照相比，在300 nm处Foxp 3诱导增加2倍。类似地，在300 nm处，白细胞介素（IL）-10诱导增加10倍。相反，在300 nm处，IL-17、IL-23和IL-12分别被抑制40%、25%和30%。这些数据表明，300-nm UV光诱导表达Foxp 3的调节性T细胞并抑制Th-17细胞。除了诱导表达Foxp 3的调节性T细胞外，Th-17细胞的抑制可能在UV光诱导的免疫抑制中起重要作用。在UVB范围内使用特定波长是诱导抗原特异性外周耐受的新策略，并为过敏性皮肤病提供了新的潜在治疗。

项目成果

今日の治療指針

今天的治疗指南

• 发表时间: 2014
• 作者: Tomonori Yamanishi;Masahiro Yashi;Miki Fuse;Hideyuki Abe;Hironori Betsuno;Hideo Yuki;Yoshitatsu Fukabori;Takao Kamai;山西友典;山西友典
• 通讯作者: 山西友典

Dermal mast cells are involved in the incidence of scratching behavior caused by multiple application of hapten,2,4,6-trinitrochlorobenzene, in mice

真皮肥大细胞与多次使用半抗原2,4,6-三硝基氯苯引起的小鼠抓挠行为的发生有关

• 发表时间: 2005
• 期刊: J Exp Dermatol 14
• 作者: Hirotaka;Yamashita;Yoshiko;Michibata;Hajime;Mizukami;Yukio;Ogihara;Akimichi;Morita;Mitsuhiko;Nose;Hirotaka Yamashita
• 通讯作者: Hirotaka Yamashita

難治性皮膚疾患の病態と治療

顽固性皮肤病的病理学与治疗

• 发表时间: 2005
• 作者: 森田 明理;上尾 礼子;森田 明理
• 通讯作者: 森田 明理

Recent developments in phototherapy: treatment methods and devices.

• DOI: 10.2174/187221308784543665
• 发表时间: 2008-05
• 期刊: Recent patents on inflammation & allergy drug discovery
• 影响因子: 4.2
• 作者: A. Morita;M. Weiss;A. Maeda

皮膚疾患最新の治療

皮肤病的最新治疗方法

• 发表时间: 2019
• 作者: Murata Teruasa;Honda Tetsuya;Egawa Gyohei;Yamamoto Yasuo;Ichijo Ryo;Toyoshima Fumiko;Dainichi Teruki;Kabashima Kenji;正木 仁・鈴木敏幸・安藤秀哉 編集;古川福実・佐伯秀久
• 通讯作者: 古川福実・佐伯秀久