Identification of suicide candidate genes in amygdala and its application to neuroimaging studies

杏仁核自杀候选基因的鉴定及其在神经影像学研究中的应用

• 批准号: 17390319
• 负责人: SHIRAKAWA Osamu
• 金额: $ 10.25万
• 依托单位: Kinki University (2007)Kobe University (2005-2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390319/
• 关键词: suicide; biology; gene polymorphism; gene expression; amygdala; neuroendocrinology; neuroimaging; cognition

1. We studied the clinical and psychological characteristics associated with suicidality based on the history of lethal suicide attempt in 76 patients mainly with mood disorders. Hopelessness rather than depression, aggression in less than 40 years old patients and neuroticism were associated with suicidality. The perspectives on death were not associated with suicidality.2. We performed the profiling of mRNA expressions in amygdala of suicide brains. The 21 increased and the 9 decreased expressions of genes were identified. Among the genes with increased expressions, the polymorphisms of 14-3-3 ε gene were associated with suicide. Any polymorphisms in genes with the decreased expressions were not associated with suicide.3. Among the functional gene polymorphisms which affect a hypothalamic-pituitary-adrenal system (HPA system), we found the significant associations of OPRM1 Asn40Asp, ACE I/D and ADEA2A C-1291G polymorphism with suicide.4. For functional MRI, we made a neuropsychological task which activates emotion. A stronger activation in amygdala and posterior cingulate gyrus was shown in case of recognition in emotionally negative words, compared with emotionally neutral words. We made the tasks comprising the recall of past negative or positive episodes and the imagination of future negative or positive episodes. The activations were found in the medial frontal cortex, dorsolateral prefrontal cortex posterior cingulate gyrus.

1.本文对76例以心境障碍为主的自杀未遂者的临床和心理特征进行了研究。与自杀倾向相关的是绝望而非抑郁、40岁以下患者的攻击性和神经质。对死亡的看法与自杀倾向无关.我们对自杀脑杏仁核的mRNA表达进行了分析。其中21个基因表达上调，9个基因表达下调。在表达增高的基因中，14-3-3 ε基因多态性与自杀相关。任何基因多态性的表达降低与自杀无关.在影响下丘脑-垂体-肾上腺系统（HPA系统）的功能基因多态性中，我们发现OPRM 1 Asn 40 Asp、ACE I/D和ADEA 2A C-1291 G多态性与自杀显著相关.对于功能性磁共振成像，我们做了一个激活情绪的神经心理学任务。与情绪中性词相比，在识别情绪消极词的情况下，杏仁核和后扣带回的激活更强。我们制定的任务包括回忆过去的消极或积极事件以及想象未来的消极或积极事件。在内侧额叶皮层、背外侧前额叶皮层、扣带回后部发现激活。

项目成果

Expression of Kruppel-like factor 5 gene in human brain and association of the gene with the susceptibility to schizophrenia

• DOI: 10.1016/j.schres.2007.11.042
• 发表时间: 2008-03
• 期刊: Schizophrenia Research
• 影响因子: 4.5
• 作者: M. Yanagi;T. Hashimoto;N. Kitamura;M. Fukutake;O. Komure;N. Nishiguchi;T. Kawamata;K. Maeda;O. Shirakawa

Association between a functional polymorphism in the renin-angiotensin system and completed suicide

• DOI: 10.1007/s00702-006-0483-9
• 发表时间: 2006-12-01
• 期刊: JOURNAL OF NEURAL TRANSMISSION
• 影响因子: 3.3
• 作者: Hishimoto, A.;Shirakawa, O.;Maeda, K.
• 通讯作者: Maeda, K.

A functional polymorphism of the micro-opioid receptor gene is associated with completed suicides.

微阿片受体基因的功能多态性与完成自杀有关。

• 期刊: J Neural Transm (in press)
• 作者: Hishimoto;A.
• 通讯作者: A.

Association of RGS2 gene Polymorphisms with suicide and increased RGS2 immunoreactivity in the postmortem brain of suicide victims

• DOI: 10.1038/sj.npp.1301557
• 发表时间: 2008-06-01
• 期刊: NEUROPSYCHOPHARMACOLOGY
• 影响因子: 7.6
• 作者: Cui, Huxing;Nishiguchi, Naoki;Shirakawa, Osamu
• 通讯作者: Shirakawa, Osamu

No evidence of an association between tyrosine hydroxylase gene polymorphisms and suicide victims.

没有证据表明酪氨酸羟化酶基因多态性与自杀受害者之间存在关联。

• 发表时间: 2006
• 期刊: Kobe J Med Sci 52
• 作者: Nahoko Nakano;Kaori Takai-Fukuhara;Tadashi Jono;Keisuke Nakajou;Nobuaki Eto;Seikoh Horiuchi;Motohiro Takeya;Ryoii Nagai;Nishiguchi N et al.;Hishimoto A et al.;Hattori H et al.
• 通讯作者: Hattori H et al.