Identification of altered gene expression in psychotomimetic-treated animals and its changes in postmortem brains from patients with schizophrenia

鉴定拟精神病药物治疗动物基因表达的改变及其在精神分裂症患者死后大脑中的变化

• 批准号: 09670989
• 负责人: SHIRAKAWA Osamu
• 金额: $ 1.79万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670989/
• 关键词: schizophrenia; phencyclidine; nRNA; phospholipase C; human brain; differential display; PCR

1. We searched for phencylidine-induced genes using mRNA differential disp1ay methods. One cDNA was indentified arid cloned in the nucleus accumbens of treated rats and the DNA sequence was analyzed in further details. This clone showed strong sequence homology to a human expressed sequence tag clone. We confirmed higher level of this mRNA in the nucleus accumbens at 24 hrs after phencylidine (8 mg/kg, s.c.) injection by nothern blot analysis. To identify the complete sequence of this mRNA, cDNA cloning is going on.2. We immunoquantified PLC beta1, gamma1 and delta1 in the left prefrontal cortex and superior temporal cortex, nucleus accumbens and amygdala, and in the right superior temporal cortex of postmortem brains obtained from a total of 19 patients with schizophrenia and a total of 28 controls. PLC beta1 immunoreactivities were increased in the prefrontal cortex, while they were decreased in the left superior temporal cortex, as compared with the values in controls. There was no difference in PLC beta1 immunoreactivities in the nucleus accumbens, the amygdala or the right superior temporal cortex between schizophrenic patients and controls. PLC gamma1 and delta1 immunoreactivities did not differ between the two groups in any of the regions studied.

1.我们用mRNA差异显示法寻找苯环哌啶诱导的基因。在处理的大鼠延髓核中鉴定并克隆了一个cDNA，并进一步详细分析了DNA序列。该克隆显示出与人表达的序列标签克隆的强序列同源性。我们证实，在苯环苯胺（8 mg/kg，s.c.）注射液通过免疫印迹分析。为了鉴定该mRNA的完整序列，正在进行cDNA克隆.我们免疫定量PLC β 1，γ 1和δ 1在左前额叶皮层和上级颞叶皮层，杏仁核和杏仁核，并在右上级颞叶皮层的死后大脑中获得的共19例精神分裂症患者和共28名对照。与对照组相比，前额叶皮质PLC β 1免疫反应性增加，而左侧上级颞叶皮质PLC β 1免疫反应性降低。精神分裂症患者和对照组之间的中脑核、杏仁核或右侧上级颞叶皮质PLC β 1免疫反应性没有差异。PLC γ 1和δ 1免疫反应性在任何研究区域的两组之间没有差异。

项目成果

Yang C.-Q.,et al: "Isotype-specific G protein abnormalities in the left superior temporal cortex and limbic structures of patients with chronic schizophrenia." Biological Psychiatry. 43・1. 12-19 (1998)

Yang C.-Q. 等人：“慢性精神分裂症患者的左上颞叶皮层和边缘结构中的同型特异性 G 蛋白异常。” 43·1 (1998)。

Yang, C.-Q., et al.: "Isotype-specific G protein abnormalities in the left superior temporal cortex and limbic structures of patients with chronic schizophrenia." Biol.Psychiatry. 43. 12-19 (1998)

Yang, C.-Q. 等人：“慢性精神分裂症患者左上颞叶皮层和边缘结构中同型特异性 G 蛋白异常。”

Kitamura,N., et al: "Asymmetrical changes in the fodrin α subunit in the superior temporal cortices in schizophrenia" Biol.Psychiatry. 43. 254-262 (1998)

Kitamura, N. 等人：“精神分裂症中颞上皮质中胞质蛋白 α 亚基的不对称变化”Biol.Psychiatry 43. 254-262 (1998)

Yong,C-Q., et al.: "Isotype-specific G protein abnormalities in the left superior temporal cortex and limbic structures of patients with chronic schizophrenia." Biol.Psychiatry. 43. 12-19 (1998)

Yong,C-Q., et al.：“慢性精神分裂症患者左上颞叶皮层和边缘结构中同种型特异性 G 蛋白异常。”

Lin,X-H.,et al: "Opposite changes in phosphoinositide-specific phospholipase C in the left prefrontal and superior temporal cortex of patients with chronic schizophrenia" Biol.Psychiatry. in press. (1999)

Lin,X-H.,et al：“慢性精神分裂症患者左前额叶和颞上皮质中磷酸肌醇特异性磷脂酶 C 的相反变化”Biol.Psychiatry。