Identification of altered gene expression in psychotomimetic-treated animals and its changes in postmortem brains from patients with schizophrenia

1.用mRNA差异显示方法寻找苯环利定诱导的基因。在处理组大鼠的伏隔核中鉴定并克隆了一个基因，并对其DNA序列进行了进一步的分析。该克隆与人表达序列标签克隆具有很强的序列同源性。我们证实，在苯环利定(8 mg/kg，S.C.)作用24小时后，伏隔核中该基因的表达水平较高。诺氏印迹分析注射剂。为确定该基因的完整序列，目前正在进行基因克隆。对19例精神分裂症患者和28例正常对照组的左侧前额叶皮质、上颞叶皮质、伏隔核和杏仁核，以及右侧颞叶上皮质的PLCβ1、Gamma1和Delta1进行了免疫定量研究。与对照组相比，前额叶皮质PLCβ1免疫反应活性增强，而左侧颞叶上皮质PLCβ1免疫反应活性降低。精神分裂症患者与对照组伏隔核、杏仁核、右侧颞叶上皮质PLCβ1免疫反应活性无明显差异。PLC-Gamma1和Delta1免疫反应活性在研究的任何区域在两组之间都没有不同。

1. We searched for phencylidine-induced genes using mRNA differential disp1ay methods. One cDNA was indentified arid cloned in the nucleus accumbens of treated rats and the DNA sequence was analyzed in further details. This clone showed strong sequence homology to a human expressed sequence tag clone. We confirmed higher level of this mRNA in the nucleus accumbens at 24 hrs after phencylidine (8 mg/kg, s.c.) injection by nothern blot analysis. To identify the complete sequence of this mRNA, cDNA cloning is going on.2. We immunoquantified PLC beta1, gamma1 and delta1 in the left prefrontal cortex and superior temporal cortex, nucleus accumbens and amygdala, and in the right superior temporal cortex of postmortem brains obtained from a total of 19 patients with schizophrenia and a total of 28 controls. PLC beta1 immunoreactivities were increased in the prefrontal cortex, while they were decreased in the left superior temporal cortex, as compared with the values in controls. There was no difference in PLC beta1 immunoreactivities in the nucleus accumbens, the amygdala or the right superior temporal cortex between schizophrenic patients and controls. PLC gamma1 and delta1 immunoreactivities did not differ between the two groups in any of the regions studied.