Development of New diagnostic methods and treatments against EB virus as a target in nasal NK/T cell lymphoma

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(1) Virological analysisWe analyzed nasal NK/T cell lymphoma cell line to investigate the role of cytokines by cDNA microarray. IL-9 was expressed in EB virus positive nasal NK/T cell lymphoma cell line but not negative cell lines. In addition, it seems that IL-9 has contributed for the cell proliferation by autocrine mechanism in the cell line. s.We analyzed gene mutation of LMP1 and LMP2A in nasal NK/T cell lymphoma tissues. Deletion of LMP1 and mutations of LMP2A were discovered in the tissuesWe analyzed chemokines in nasal NK/T cell lymphoma cell line by the antibody array. IP-10 was expressed in nasal NK/T cell lymphoma cell line. In addition, it seems that IP-10 has contributed for the cell migration by autocrine mechanism.(2) Development of new diagnostic methodsWe developed a real time PCR method to measure EBV DNA load in sera of nasal NK/T cell lymphoma patients. Serum EBV DNA load was useful for diagnosis of this disease and to predict patient's prognosis and recurrence.(3) Development of new treatment methodsIntra-arterial chemotherapy and radiotherapy we developed was useful and safety to treat nasal NK/T cell lymphoma.Helper T lymphocytes were developed using LMP1 peptides. The T cells recognize a peptide (LMP1 159-175) with promiscuous epitopes of LMP1 and restricted HLA-DR9, DR53 and DR15. In addition, the T cells killed lymphoma cells.