Molecular biologic, immunogenetic, and Epstein-Barr virus studies on nasal NK/T-cell lymphoma

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Mutations of p53 tumor suppressor gene, b-catenine gene, and ras gene, expressions of p53 protein and b-catenine, and clinical outcome were analyzed in 32 patients with nasal NK/T-Cell lymphoma. Of 32 patients, 6 mutations (2 silent and 4 missense) of p53 gene, 5 mutations (3 silent and 2 missense) of b-catenine gene, and each 1 mutation of N- and K-ras gene were detected. Overexpression of p53 parotein and b-catenine protein were found in 15 and 4 patients, respectively. The patients with mutations of these genes showed poor prognosis. Multivariate analysis revealed p53 gene muation as a most independent pronostic factor.Direct sequence analyzes for total region of LMP-1 and the exon 6 of LMP-2A in Epstein-Barr vius gene were performed using PCR-amplified products in 7 patients with nasal NK/T-cell lymphoma. A 30-bp deletion in the 3' C-terminal region of the LMP-1 gene was detected in all 7 samples. In the LMP-1 genes, 43 similar nucleotide changes were detected in all 7 patients and 11 sequence variations were found among 7 patients. With regard to the sequence of in exon 6 of LMP-2A gene, a similar nucleotide change (AGC to ACC, serine to threonine) at codon 348 was determined in all 7 patients.