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IMMUNOLOGIC, GENETIC AND EPSTEIN-BARR VIROLOGICAL STUDIES OF MALIGNANCIES IN HEAD AND NECK LESION

头颈病变恶性肿瘤的免疫学、遗传学和 Epstein-Barr 病毒学研究

基本信息

批准号：
08671976
负责人：
HARABUCHI Yasuaki
金额：
$ 1.47万
依托单位：
ASAHIKAWA MEDICAL COLLEDE (1998)Sapporo Medical University (1996-1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

1) First, we investigated phenotypic, genotypic and Epstein-Barr virus (EBV) features of nasal T-cell lymphomas (NTL). We found that that NTL may be derived from the lineage of NK or N K-like T-cells and that EBV may play a role in the lymphomagenesis.2) The levels of soluble intercellular adhesion molecule-1 (ICAM-1) were significantly higher in NTL than that in other head and neck lymphomas as well as that in healthy adult controls. The intense expression of the ICAM-1 was found in the tissues from NTL.These data may be one of the clue to an understanding of this particular lymphoma.3) Analyses of prognostic factors non-Hodgkin's lymphoma of Waldeyer's ring revealed that prognosis was associated with primary disease in the base of tongue, stage III-IV, B-symptoms, highly histologic grade, T-cell phenotype, elevated LDH levels, decreased peripheral blood lymphocytes counts, or negative response on delayed type hypersensitivity skin reactions. Multivariate analysis showed that both stage III-IV and T-cell phenotype were significant independent risk factor for death.4) Finally, we examined the presence of EBV in the lymphomas of sino-nasal region and determined its relation with clinical, phenotypic, and genotypic features. Twenty-three patients with nasal lymphomas had pan T-antigens MT1 and/or CD2 ; while all 7 sinus lymphomas displayed pan B-antigen L26. EBER transcripts were identified in 20 of 23 patients with NTL, but was never detected in sinus lymphomas. The natural killer (NK) cell phenotype CD56, monoclonal EBV genome, EBNA1, and LMP1, were detected in all EBER-positive NTL tested. The rearrangements of T-cell receptor (TCR) genes were shown in 11 of 1 3 NTL tested. NTL showed significantly lower survival rates as compared to the other types of sino-nasal lymphomas.

1)首先，我们研究了鼻T细胞淋巴瘤（NTL）的表型，基因型和EB病毒（EBV）的特点。结果表明，NTL可能来源于NK细胞或NK样T细胞，EBV可能参与了NTL的淋巴瘤发生过程。2）NTL中可溶性细胞间粘附分子-1（ICAM-1）水平显著高于其他头颈部淋巴瘤及正常对照组。3）对影响非霍奇金淋巴瘤预后的因素进行分析，结果表明：原发于舌根、Ⅲ ~ Ⅳ期、B症状、高组织学分级、T细胞表型、LDH水平升高、外周血淋巴细胞计数降低，或迟发型超敏反应皮肤反应阴性。多因素分析显示，III-IV期和T细胞表型均为死亡的独立危险因素。4）最后，我们检测了鼻窦区淋巴瘤中EBV的存在，并确定其与临床、表型和基因型特征的关系。23例鼻淋巴瘤患者有泛T抗原MT 1和/或CD 2，而所有7个鼻窦淋巴瘤显示泛B抗原L26。23例NTL患者中有20例发现了EBER转录本，但在鼻窦淋巴瘤中从未检测到。在所有EBER阳性NTL中检测到自然杀伤（NK）细胞表型CD 56、单克隆EBV基因组、EBNA 1和LMP 1。13例NTL中有11例出现TCR基因重排。NTL显示出显着较低的生存率相比，其他类型的鼻窦鼻淋巴瘤。