Effect of mechanical stress on responses of oxidative stress

机械应力对氧化应激反应的影响

• 批准号: 17500415
• 负责人: NOMURA Jun
• 金额: $ 2.31万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17500415/
• 关键词: cell stretching; cDNA microarray; panel of antibody; fibroblast; mechanical stress; MAP kinase; cell adhesion; oxidative stress; メカノストレス; 培養ヒト線維芽細胞; プロテオーム解析; 細胞骨格; シャペロン分子; cDNA array

Oxidative stress have been implicated in a variety of human disorders including cancer and aging. We reported that exposure to mechanical stress increased urinaly 8-OHdG levels. Moreover repetitive exposure of mechanical stress reduced the 8-OHdG level These observations suggested that repetition of mechanical stress induced a tolerance. However, there are no reports about tolerance mechanisms about tolerance of mechanical stress.In this study, we analyzed relationship of responsive mechanisms between mechanical stress and oxidative stress. Mechanical stress is recognized as an important extracellular stimulus that promotes cellular growth and survival, influences metabolic processes (including gene expression), and governs tissue architecture in various cell types. Fibroblast cells were cultured on collagen coated elastic silicon chamber. And then, uni-axial stretch was applied to the cells by a computer-controlled stretching apparatus. Cells were also exposed to an oxidative stress by addition of hydrogen-oxide to the medium. Expression levels of genes and proteins were analyzed by cDNA array nad western blotting methods. We identified several differentially expressed genes and proteins, including the ER stress marker glucose-regulated protein 78 (GRP78) and ERK1. Moreover, knockdown of one of tetraspani web moles induced detachment of cells from a culture apparatus.Therefore, molecules which responsive to the oxidative stress during exposure to the mechanical stress, related to the regulation of protein expression.

氧化应激与包括癌症和衰老在内的多种人类疾病有关。我们报道了暴露于机械应力增加尿8-OHdG水平。此外，重复机械应力暴露降低8-OHdG水平。这些观察结果表明，重复机械应力诱导耐受。然而，目前还没有关于机械应激耐受机制的报道，本研究分析了机械应激与氧化应激之间的反应机制关系。机械应力被认为是促进细胞生长和存活、影响代谢过程（包括基因表达）并支配各种细胞类型中的组织结构的重要细胞外刺激。将成纤维细胞培养在胶原包被的弹性硅小室上。然后，通过计算机控制的拉伸装置对细胞施加单轴拉伸。通过向培养基中添加氢氧化物，细胞也暴露于氧化应激。用cDNA芯片和蛋白质印迹法分析基因和蛋白质的表达水平。我们确定了几个差异表达的基因和蛋白质，包括ER应激标志物葡萄糖调节蛋白78（GRP 78）和ERK 1。此外，敲除其中一只四跨膜鼹鼠可诱导细胞脱离培养装置，因此，在机械应力暴露过程中，对氧化应激反应的分子与蛋白质表达的调控有关。

项目成果

Enhanced expression of Transsferrin receptor confers UV-resistance in human and monkey cells

转铁蛋白受体表达增强赋予人和猴细胞抗紫外线能力

• 发表时间: 2005
• 期刊: Journal of Radiation Research 46
• 作者: Chen;Z-H.
• 通讯作者: Z-H.

Modulation of mutability in UV-irradiated human RSb cells by serum obtained from parabolic flight volunteers

抛物线飞行志愿者血清对紫外线照射的人类 RSb 细胞突变性的调节

• 发表时间: 2007
• 期刊: J. Int. Soc. Life Info. Sci. 25
• 作者: Chi;X.;Takahashi;S.;Nomura;J.;Sugaya;S.;Ishimura;Y.;Zhai,L.;Tong;X.;Kita;K.;Suzuki;N.
• 通讯作者: N.

周期的伸展後のケロイド由来皮膚線維芽細胞で誘発される遺伝子の解析

循环拉伸后瘢痕疙瘩源性真皮成纤维细胞诱导基因分析

• 发表时间: 2006
• 作者: Nomura;J;et. al.;野村 純;金沢 雄一郎
• 通讯作者: 金沢 雄一郎

細胞伸展により誘導される遺伝子のマイクロアレイ解析

细胞扩散诱导基因的微阵列分析

• 发表时间: 2005
• 作者: Kanazawa;Y;et. al.;金沢 雄一郎
• 通讯作者: 金沢 雄一郎

Modulation of mutability in UV-irradiated human RSb cells by serum obtained parabolic flight volunteers

抛物线飞行志愿者血清对紫外线照射的人类 RSb 细胞突变性的调节

• 期刊: Journal of International Society of Life Information Science (In press)
• 作者: Chen;Z-H.;Xio-Jun Chi
• 通讯作者: Xio-Jun Chi