权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

A Basic Resear honFunctional Pmpafie of Biodeg,adable Polymers(mainly Antifeeze Protdn) and its applications

生物可降解聚合物（主要是防霉剂）的功能性产品基础研究及其应用

基本信息

批准号：
17500536
负责人：
MIYOSHI Emako
金额：
$ 2.46万
依托单位：
Osaka University (2007)Osaka University of Foreign Studies (2005-2006)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Recently, with increasing of the global environmental problems, greater efforts have been made to develop the effective usage of biomass having various functional properties, because these are environmentally degradable and bioabsorbable. Many research groups have already reported the characteristics of antifreeze protein (AFP) from fish, however the physicochemical properties of AFP have not been clarified sufficiently and still the subject of extensive investigation.We have isolated a Type II AFP from Japanese smelt (its blood and muscle) and characterized the physicochemical properties of the AFP. The molecular weight of the AFP determined by TOF-Mass was 16756.8. The N-terminal amino acid sequence of Japanese smelt AFP was 75.0% identical to Type II AFP from herring.We have tried to develop the helpful usage of AFP for food application. Therefore, we have monitored effects of AFP on gelatinization and retrogradation of various starches, using rheological measurement and differentia … More l scanning calorimetry (DSC). Because great efforts have been made to keep the state of starch-based products to that just after gelatinization, or to control the retrogradation of starch in these foods, with increasing use of processed and simulated foods.Investigation of retrogradation process showed that the addition of AFP of fairly low concentration (10 μg/ml) significantly influenced the retrogradation process of both maize and waxy maize starches. As for maize starch, the addition of 10 μg/ml AFP significanty retarded both the initial gelation and further recrystallization. However, as for waxy maize starch, this obviously promoted the initiation of recrystallization but retarded the further recystallization.Moreover, 10μg/ml AFP also influence the rheological and thermal properties of gellan gum aqueous solutions.The results of Bioelectrical Impedance Spectroscopy illustrated that AFP with this concentration (10 μg/ml) had high inhibition activity against the ice recrystallization in comparison with those with other concentration.However, the present data were obtained only from a few experimental measurements, indeed, more detailed studies based on other methods to describe the phenomena at the molecular level are required. Less

近年来，随着全球环境问题的日益严重，人们更加努力地开发各种功能性质的生物质的有效利用，因为这些生物质具有环境可降解性和生物可吸收性。许多研究小组已经报道了鱼类抗冻蛋白(AFP)的性质，但AFP的物理化学性质仍未得到充分阐明，仍是广泛研究的课题。飞行时间质谱仪测得甲胎蛋白的相对分子质量为16756.8。日本产AFP的N端氨基酸序列与鲱鱼的II型AFP的氨基酸序列同源性为75.0%，为进一步开发AFP在食品中的应用奠定了基础。因此，我们通过流变学测量和DISTRANAS…检测了甲胎蛋白对不同淀粉糊化和回生的影响。更多的是L的扫描量热仪。因为随着加工食品和模拟食品的使用越来越多，人们一直在努力将淀粉类产品保持在糊化后的状态，或控制这些食品中淀粉的回生过程。对回生过程的研究表明，较低浓度(10μg/ml)的甲胎蛋白的添加对玉米和糯玉米淀粉的回生过程都有显著影响。对于玉米淀粉，添加10μg/mlAFP显著延缓了玉米淀粉的初凝胶化和进一步重结晶。此外，10μg/mlAFP对结冷胶水溶液的流变性和热学性质也有影响。生物交流阻抗谱分析结果表明，与其他浓度的AFP相比，该浓度(10μg/ml)的AFP对冰重结晶具有较高的抑制活性。然而，目前的数据仅来自少数实验测量，确实需要基于其他方法在分子水平上描述这种现象的更详细的研究。较少