Development of New Anti-diabetic Food using Ukogi Leaf Activities.

以动物和健康志愿者为实验对象，研究了木叶茶的抗糖尿病作用。首先，以α -葡萄糖苷酶抑制活性为指标，考察了木叶茶的提取方法。热水浸提后，木香叶茶的α -葡萄糖苷酶抑制活性增强。在正常大鼠糖耐量试验中，乌木叶茶明显抑制口服麦芽糖或淀粉后血糖水平的升高。以自发性2型糖尿病小鼠为实验对象，观察长期饮用木叶茶对小鼠空腹血糖和HbAlc水平的抑制作用。持续摄入木叶茶可显著抑制实验期间空腹血糖升高，显著降低HbAlc水平。对健康志愿者口服木叶茶，测定其餐后血糖水平。乌木叶茶抑制了摄食后30min血糖水平的升高。在受试者中观察到明显的乌木叶茶的抑制作用，其进餐后30分钟的血糖水平高于其平均值。这些结果表明，摄入ukogi叶茶可以通过抑制胃肠道中糖的消化来防止餐后血糖水平的升高。受试者餐后血糖水平迅速升高，连续12周每天饮用木叶茶。连续饮用木叶茶后，受试者餐后血糖升高明显受到抑制。这些结果表明，木叶茶可能对糖尿病的预防非常有用，也可能是饮食治疗的补充手段。

The antidiabetic effects of ukogi leaf tea were investigated in animal or healthy volunteer subjects. Firstly, extracting methods of ukogi leaf tea were examined using inhibitory activity of alfa-glucosidase. The alfa-glucosidase inhibitory activity of ukogi leaf tea was strengthened by extracting with hot water, which contains 2% ukogi leaf. In sugar tolerance tests in normal rats, ukogi leaf tea significantly suppressed the increase of blood glucose level after oral administration of maltose or starch. The inhibitory effects of long term intake of ukogi leaf tea on fasting blood glucose and HbAlc level were examined using KKAy mice which develop type2 diabetes spontaneously. Continual intake of ukogi leaf tea suppressed significantly the elevation of fasting blood glucose level during experiment and decreased level of HbAlc markedly. In oral administration of ukogi leaf tea to healthy volunteer subjects, the postprandial blood glucose level after ingestion of boiled rice was measured. Ukogi leaf tea suppressed the increase of blood glucose level at 30 min after the meal ingestion. The suppressive effect of ukogi leaf tea was observed markedly in subjects, whose blood glucose levels at 30 min after the meal ingestion were higher than their mean values. These results suggest that the intake of ukogi leaf tea prevents increases in the blood glucose level after a meal by inhibiting digestion of sugars in gastrointestinal tract. The subjects, whose blood glucose levels after meal ingestion elevate readily, intaked ukogi leaf tea everyday for 12 weeks. After the continual intake of ukogi leaf tea, the postprandial blood glucose elevation in the subjects was suppressed significantly. These results indicate that ukogi leaf tea might be very useful for the prevention of diabetes as well as a supplementary means for dietary therapy.