Novel endothelial cell culture substrate of polystyrene derivatives

通过在聚苯乙烯表面引入磺酸、季铵盐和羟基等功能基团，制备了聚苯乙烯衍生物，用X-射线衍射仪分析了它们的表面性质结果表明，含血清和血清-聚苯乙烯表面均无内皮细胞粘附，而含血清和血清-聚苯乙烯表面均无内皮细胞粘附。在含血清条件下，内皮细胞在磺化聚苯乙烯表面的粘附性高，与磺酸含量无关。在无血清条件下，磺化聚苯乙烯表面的内皮细胞粘附率随磺酸含量的增加而增加，在季铵盐处理的聚苯乙烯表面也表现出类似的行为，这些表面表现出粘附的内皮细胞的增殖，其程度几乎与组织培养相同-这些结果表明，内皮细胞的粘附和增殖可以通过基底的表面特性来控制。

Culture of human endothelial cells on the surfaces of polystyrene derivatives was examined in terms of cell adhesion cell proliferation and production of prostacycline on these surfaces.Polystyrene derivatives were prepared by introducing functional groups such as sulfonic acid, quartery ammonium salt and hydroxyl-group to polystyrene surface, and their surface properties were analyzed by X-ray photoelectron spectroscopy and dynamic contact angle measurement.Few endothelial cell adhered on polystyrene surface in both case of serum-containing and serum-free condition.In case of serum-containing condition,adhesion of endothelial cells on sulfnated polystyrene surface was high, independent of the content of sulfonic acid. However in case of serum-free condition adhesion of endothelial cells on sulfonated polystyren surface increased with the content of sulfonic acid.The similar behavior was also observed on the polystyrene surface with quartery ammonium salt.These surfaces exhibited the proliferation of adhering endothelial cells, the extent of which was almost the same as tissue culture-graded polystyrene.These results suggest that adhesion and proliferation of endothelial cells could be controlled by surface characteristics of substrates.