Novel endothelial cell culture substrate for angiogenesis

采用固相法合成了两种两性聚合物，即聚(苯乙烯-甲基丙烯酸二乙氨基乙酯)无规共聚物和聚(苯乙烯-氯甲基苯乙烯对苯磺酸钠)无规共聚物。合成了含磺酸、季铵盐、羟基等官能团的聚苯乙烯衍生物。将这些聚合物涂覆在玻璃表面，通过X射线光电子能谱和动态接触角测量分析其表面性质，并在这些表面上培养人内皮细胞，观察细胞的黏附、细胞增殖、细胞形态和前列环素的产生。细胞可以在含有磺酸和两性离子的表面上黏附。这些结果表明，叔胺或磺酸等官能团参与了细胞的早期黏附。此外，细胞的增殖能力和前列环素的产生能力取决于官能团的种类和含量。细胞增殖刺激的表面也促进了前列环素的产生。

We developed novel endothelial cell culture substratum for angiogenesis.Two ampholyte polymers, poly (styrene-diethylaminoehtyl methacrylate) random copolymer and poly (styrene-chrolomethl styrene sodium p-styrene sulfonate) random copolymer, were synthesized by solid phase method. and polystyrene derivatives incorporated with functional groups such as sulfonic acid, quartery ammonium salt and hydroxyl-group were preparated. These polymers were coated on glass surfaces and their surface properties were analyzed by X-ray photoelectron spectroscopy and dynamic contact angle measurement.Human endothelial cells were cultured on these surfaces and cell adhesion, cell proliferation, cell morphology and prostacycline production were examined.Cells could adhere on surfaces with sulfonic acid and ampholyte. These results suggest that functional groups such as tertiary amine or sulfonic acid were participated in early cell adhesion. Moreover, abilities of cell proliferation and prostacycline production were depended on variety and content of functional groups. The surfaces which cell proliferation stimulated were also promoted prostacycline production.