Combined effects of light and naphthalene on marine microalgae

光和萘对海洋微藻的联合影响

• 批准号: 17510034
• 负责人: OKUMURA Yutaka
• 金额: $ 2.44万
• 依托单位: Fisheries Research Agency
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17510034/
• 关键词: photo induced toxicity; xanthophyll cycle; microalgae; ultra violet; high light; 植物プランクトン; 光毒性

In this study, I investigated the combined effects of light and naphthalene on marine microalgae. The growth of many groups of phytoplankton is strongly inhibited by combined high light irradiation(2000 μmol m^<-2> s^<-1>) and naphthalene(0.05-5 ppm) because of the combined effects of photoinhibition and toxicity. The xanthophyll cycle of microalgae is thought to protect against photoinhibition : light in excess of photosynthetic capacity is known to rapidly change xanthophyll pigments of green algae from violaxanthin via antheraxanthin to zeaxanthin, and those of diatoms from diadinoxanthin to diatoxanthin. Irradiation of microalgae at 2000 μmol m^<-2> s^<-1> during exposure to naphthalene at >15ppm caused the fastest change in xanthophyll pigments among four conditions(200 or 2000 μmol m^<-2> s^<-1> and 0 or >15 ppm naphthalene). Naphthalene decreased the production of dissolved oxygen, an indicator of photosynthesis. Therefore naphthalene inhibited photosynthesis, and rapidly activated the xanthophyll cycle. Microalgae produce mycosporine-like amino acids(MAAs), whose absorbance maxima lie in the ultraviolet(UV) region, to protect against inhibition by UV radiation. The production of dissolved oxygen was decreased by the synergistic effects of UV-A(~360 nm), high light irradiation, and naphthalene. It was not clear whether the ratio of MAAs to chlorophyll a was changed by UV-A and naphthalene. It appears that the production of MAAs in microalgae used in this experiment was not influenced by UV-A and naphthalene.

在这项研究中，我研究了光和萘对海洋微藻的联合影响。由于光抑制和毒性的综合作用，强光照射(2000ppm~(-2)μ；S~(-1)~(-1))和萘(0.05~5ppm)对多种浮游植物的生长都有强烈的抑制作用。微藻的叶黄素循环被认为是对光抑制的保护：已知超过光合作用能力的光可以迅速将绿藻的叶黄素从紫黄质通过花黄素转变为玉米黄质，而硅藻的叶黄素则从双黄质转变为双黄质。在2 0 0 0或2 0 0 0μμm；-2&gt；S和0或&gt；15ppm的萘照射下，微藻叶黄素色素的变化最快。萘减少了溶解氧的产生，而溶解氧是光合作用的指标。因此，萘抑制了光合作用，并迅速激活了叶黄素循环。微藻产生类霉孢素氨基酸(MAAs)，其最大吸收位于紫外线(UV)区域，以保护其免受紫外线辐射的抑制。UV-A(~360 nm)、强光照射和萘的协同作用降低了溶解氧的产生。目前尚不清楚UV-A和萘是否改变了MAAs与叶绿素a的比值。可见，UV-A和萘对本实验所用微藻中MAAs的产生没有影响。