Analysis of Thermotolerance of Cancer by Introduction of Oncogeges

通过引入癌基因来分析癌症的耐热性

• 批准号: 07671015
• 负责人: OKUMURA Yutaka
• 金额: $ 1.47万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671015/
• 关键词: Oncogene; Thermosensitivity; NIH3T3 Cells

Hyperthermia is introduced as a new modality of cancer treatment. As thermosensitivity varies to tumors in ten times, all tumors can not be applied to hyperthermia. Oncogenesis of cells is induced by expression of mutated oncogenes. We introduced oncogenes to normal cells and obtained transformed cells, and examined thermosensitivity of cells, analyzing oncogenes which determine thermosensitivity. This study will predict results of cancer treatment by hyperthermia before its treatment, and most effective treatment would be selected.In this study, mouse cultured cells, NIH3T3, were used for normal cells. Following oncogenes were introduced to normal cells and obtained transformed cells : myc, Ki-ras, N-ras, H-ras, int-2, fgr, sis, and src.Thermosensitivity of transformed cells as well as normal cells to 44ﾟC was examined. The transformed cells by Ki-ras showed resistant to hyperthermia. This means that tumors induced by Ki-ras is not desirable to treat by hyperthermia.Thermotolerance induced by the treatment of 44ﾟC,for 15 min was examined for above transformed cells. The thermotolerant ratio analyzed at 4 hour-interval was 2.06 for transformed cells by Ha-ras which was the smallest in examined cells. This means that tumors induced by Ha-ras is desirable to treat by fractionated hyperthermia.

热疗作为一种新的癌症治疗方式被引入。由于肿瘤的热敏感性相差十倍，因此不能对所有肿瘤进行热疗。细胞的肿瘤发生由突变的癌基因的表达诱导。我们将癌基因导入正常细胞并获得转化细胞，并检查细胞的热敏性，分析决定热敏性的癌基因。本研究将在治疗前预测热疗治疗癌症的效果，并选择最有效的治疗方法。在本研究中，小鼠培养细胞NIH 3 T3被用作正常细胞。将myc、Ki-ras、N-ras、H-ras、int-2、fgr、sis和src等癌基因导入正常细胞，获得转化细胞，并检测转化细胞和正常细胞对44 ℃的温度敏感性。Ki-ras基因转化的细胞对高温有抵抗作用。用44 ℃处理上述转化细胞15分钟，观察其耐热性。Ha-ras转化细胞每隔4小时分析的耐热率为2.06，这是受检细胞中最小的。这意味着由Ha-ras诱导的肿瘤需要通过分次热疗来治疗。

项目成果

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H.Kondo：“辐射暴露与日本放射学技术人员的临床检查数据之间的统计关联。”