Fabrication of Inorganic-Organic Hybrid Membrane with Ion-Channel Function

细胞膜上的离子通道是维持生物体内稳态的重要信号转导途径。离子通道作为一种信号转导器，不仅在生物学领域，而且在材料化学领域都有着广泛的应用，因为这种功能对于传感器件的元件是非常有用的。但其作用机制尚不明确。为了阐明这一机理，本工作尝试制备具有离子通道功能的人工膜，选择了冠醚衍生物和杯衍生物等人工离子载体，因为它们已被报道在单个分子中显示出离子通道功能，并且这些离子载体的结构修饰是可能的。这些性质有利于研究其作用机理。为了提高产率，我们考察了各种合成路线，并建立了一条规模较大的合成路线。我们认为，类似于脂双层膜的性质是人工膜诱导连接载体离子通道功能所必需的。因此，合成的膜材料组成的磷酸胆碱，低聚乙二醇，和烷硫醇的人工膜的制造进行了检查。在磷酰氯存在下，寡聚乙二醇衍生物与胆碱的反应提供了所需的膜材料。结果表明，用合成的膜材料制备的自组装膜对蛋白质的非特异性吸附具有抑制作用，虽然目前还没有制备出具有离子通道功能的人工膜，但这些膜材料有可能成为生物相容性材料，如重组膜。

Ion-channel in cell membrane is known to work as signal transduction to contribute to biological maintenance of homeostasis. The function of ion-channel as a signal transducer is attractive not only in biology but also in material chemistry field because this function is useful for elements of sensing devices. However, its action mechanism is still ambiguous. In order to shed light on this mechanism, fabrication of an artificial membrane with ion-channel function was attempted in this work.Artificial ionophores such as crown ether derivatives and calix derivatives were chosen as they have been reported to show ion-channel function with single molecule and structure modification of those ionophores are possible. Those properties must be of advantage to study the action mechanism. Various synthesis routes were examined in order to improve yields, and a relatively large-scale synthesis route was established.We consider that the nature, similar to lipid bilayer membrane, is necessary for the artificial membrane to induce ion-channel function of an jonophore. Therefore, synthesis of membrane materials consisting of phospho choline, oligo ethylene glycol, and alkane thiol was examined for fabrication of the artificial membrane. The reaction of oligo ethylene glycol derivatives with choline in the presence of phosphoryl chloride afforded the desired membrane materials. It was found that the SAMs fabricated with the synthesized membrane materials show suppressive function for non-specific adsorption of proteins.It is suggested that those membrane materials have a potential for biocompatible materials such as reconstituted membrane although fabrication of an artificial membrane with ion-channel function has not been reached.