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Fabrication of Inorganic-Organic Hybrid Membrane with Ion-Channel Function

具有离子通道功能的无机-有机杂化膜的制备

基本信息

批准号：
17550140
负责人：
TANAKA Mutsuo
金额：
$ 2.43万
依托单位：
National Institute of Advanced Industrial Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2008
项目状态：
已结题

项目摘要

Ion-channel in cell membrane is known to work as signal transduction to contribute to biological maintenance of homeostasis. The function of ion-channel as a signal transducer is attractive not only in biology but also in material chemistry field because this function is useful for elements of sensing devices. However, its action mechanism is still ambiguous. In order to shed light on this mechanism, fabrication of an artificial membrane with ion-channel function was attempted in this work.Artificial ionophores such as crown ether derivatives and calix derivatives were chosen as they have been reported to show ion-channel function with single molecule and structure modification of those ionophores are possible. Those properties must be of advantage to study the action mechanism. Various synthesis routes were examined in order to improve yields, and a relatively large-scale synthesis route was established.We consider that the nature, similar to lipid bilayer membrane, is necessary for the artificial membrane to induce ion-channel function of an jonophore. Therefore, synthesis of membrane materials consisting of phospho choline, oligo ethylene glycol, and alkane thiol was examined for fabrication of the artificial membrane. The reaction of oligo ethylene glycol derivatives with choline in the presence of phosphoryl chloride afforded the desired membrane materials. It was found that the SAMs fabricated with the synthesized membrane materials show suppressive function for non-specific adsorption of proteins.It is suggested that those membrane materials have a potential for biocompatible materials such as reconstituted membrane although fabrication of an artificial membrane with ion-channel function has not been reached.

细胞膜上的离子通道被认为是一种信号转导途径，有助于生物维持体内平衡。离子通道作为一种信号换能器的功能不仅在生物学领域，而且在材料化学领域也很有吸引力，因为它对传感器件的元件很有用。然而，其作用机制仍不明确。为了阐明这一机制，本工作尝试了一种具有离子通道功能的人工膜的制备。根据已报道的人工离子载体，如冠醚衍生物和杯芳衍生物，它们可以显示单分子的离子通道功能，并且这些离子载体的结构修饰是可能的。这些性质对研究其作用机理具有一定的参考价值。为了提高产率，考察了不同的合成路线，并建立了较大规模的合成路线。我们认为，人工膜的性质类似于脂质双层膜，需要人工膜来诱导离子通道功能。因此，为了制备人工膜，对由磷胆碱、低聚乙二醇和烷基硫醇组成的膜材料的合成进行了研究。在磷酰氯存在下，低聚乙二醇衍生物与胆碱反应得到所需的膜材料。结果表明，合成的自组装膜材料对蛋白质的非特异性吸附具有抑制作用，尽管目前还没有制备出具有离子通道功能的人工膜，但仍有可能成为重组膜等生物相容性材料。