Analysis of the distribution and population composition dynamics of red tide-causing microalgae based on their genomic polymorphism

基于基因组多态性的赤潮微藻分布及种群组成动态分析

基本信息

批准号：
17580168
负责人：
YOSHIKAWA Takeshi
金额：
$ 2.04万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The aim of this research is to establish genetic markers to identify a red-tide causing raphidophyte Heterosigma akashiwo intraspecifically.1. The random amplified polymorphic DNA (RAPD) technique detected genomic polymorphism among H. akashiwo strains. Some of sequence-tagged site (STS) primers designed according to the amplicon sequences detected H. akashiwo strains specifically by means of PCR amplification.2. The microsatellite-primed PCR detected genomic polymorphism among H. akashiwo strains. Some of STS primers targeting to the microsatellites found in the amplicon detected H. akashiwo strains specifically by PCR amplification.3. Nucleotide sequences between 16S rDNA and rbcL encoded on the H. akashiwo plastidal DNA, and between rbcL and cfxQ encoded on the plastidal DNA of Chattonella species, which belong to the family Raphidophyceae as well as H. akashiwo, were determined. The Chattonella species showed no sequence differences at the intergenic region between rbcL and rbcS.4. A model of red tide blooms was constructed by adding H akashiwo cells to coastal seawater, and the environmental DNA was prepared. Some of the genetic markers established on the H akashiwo plastidal DNA (Signature A-E, Akase, et. al., 2004) detected H. akashiwo strains specifically from the environmental DNA, suggesting availability of these markers for intraspecific detection of H. akashiwo strains or populations from marine natural environments.

这项研究的目的是建立遗传标志物，以识别引起施法的杂化杂质akashiwo的红潮。1。随机扩增的多态性DNA（RAPD）技术检测到Akashiwo菌株中的基因组多态性。根据PCR扩增，基于Amplicon序列设计的一些序列标签位点（STS）引物（STS）引物。2。微卫星培训的PCR检测到了Akashiwo菌株中的基因组多态性。某些靶向在检测到H. akashiwo菌株的agplicon中发现的微卫星的STS引物。3。在Akashiwo质体DNA上编码的16S rDNA和RBCL之间的核苷酸序列，以及属于属于家族的Raphidophyceae的质体DNA的RBCL和CFXQ之间的RBCL和CFXQ之间的核苷酸序列，以及Akashiwoh。Akashiwo。查顿氏菌种在RBCL和RBC之间的基因间区域没有序列差异。4。通过将H akashiwo细胞添加到沿海海水中，并制备了环境DNA来构建红色潮汐花朵的模型。在H akashiwo塑料DNA上建立的一些遗传标记（签名A-E，Akase等，2004年）检测到H. akashiwo菌株特别是从环境DNA中检测到的，这表明这些标记物可用于对H. akashiwo菌株或海上天然环境的种内检测。