腎糸球体の再生過程における糸球体内皮細胞の役割に関する検討

为了揭示人类肾小球的发生，我们评估了肾小球的发育过程以及胎儿和婴儿肾脏中CD31、CD34、FB21、α -平滑肌肌动蛋白（α - sma）和肾素的染色。我们在4个发育阶段（v期、s期、c期和m期）观察肾小球的发育过程，并观察各组肾小球CD31、CD34、FB21、α - sma和Nephrin染色。a组V期比例最高，20周后V期比例逐渐降低。B、C组S期和C期比例高于D、E、f组，m期比例最高。妊娠25周以上胎儿肾脏内皮细胞CD31、CD34和FB21染色相似。首先在s型体的下裂隙中发现系膜细胞中有α - sma染色。在s型体中，上皮细胞中也有较弱的Nephrin染色。这些结果表明，肾小球在妊娠25-34周从V期成熟到m期，内皮细胞在25周开始成熟，在35周完成。上皮细胞和系膜细胞在s期首先出现。另一方面，为了明确血清HGF浓度和肾HGF受体（HGFR）表达是否可以预测肾功能障碍的预后，我们评估了HGF浓度、HGFR- met和a -SMA在HSPN无新月形成和有新月形成患者中的表达情况。这些患者根据第一次活检的病理结果分为两组。1组为ISKDC I、II型患者20例，2组为ISKDC III、VI、v型患者35例。2组肾小球和间质α - sma染色平均评分显著高于1组。第二次活检时，2组c-Met阳性染色平均评分低于1组。我们的研究结果表明，第一次活检时肾小球和间质α - sma的表达可以预测HSPN患儿的预后，第二次活检时肾小球c-Met的表达可能与肾脏的繁殖有关。少

To reveal the human glomerulogenesis, we evaluated the process of development of glomeruli and staining for CD31, CD34, FB21, alfa-Smooth Muscle Actin (alfa-SMA), and nephrin in fetal and infant kidneys. We investigated the process of glomerular development in four developmental stages[V-stage, S-stage, C-stage and M-stage]and glomerular CD31, CD34, FB21, alfa-SMA, and Nephrin staining in each group. V-stage proportion was highest in Group A. After 20 weeks, V stage proportion gradually decreased. S- and C-stage proportions were higher in Groups B and C than in Groups D, E and F. M-stage proportion was highest in Group F. CD31, CD34 and FB21 were stained similarly in for endothelial cells in the kidneys of fetuses at over 25 weeks of gestation. Alfa-SMA stained in mesangial cells were found to the lower clefts of the S-shaped bodies first of all. Similarly Nephrin stained in epithelial cells were first observed in S-shaped body weakly. These results suggest that the glomerulus matures … More to M-stage from V stage from 25-34 weeks of gestation and that maturation of endothelial cells starts at 25 weeks and is completed by 35 weeks in the fetus. Epithelial cells and mesangial cells are appeared first at the S-stage.On the other hand, to clarify whether serum concentration of HGF and renal HGF receptor (HGFR) expression can predict the prognosis of renal dysfunction, we evaluated serum concentration of HGF and the expression of HGFR-MET and a -SMA in HSPN patients without crescent formation and with crescent formation. These patients were divided into two groups based on pathological findings on the first biopsy. Group 1 consisted of 20 patients with ISKDC I or II, while group 2 consisted of 35 patients with ISKDC III or VI or V. The mean scores of glomerular and interstitial alpha-SMA staining in group 2 were significantly higher than those in group 1. At second biopsy, the mean scores of c-Met positive staining in group 2 were lower than those in group 1. Our results suggest that the expression of glomerular and interstitial alpha-SMA at first biopsy can predict the prognosis of children with HSPN and the expression of glomerular c-Met at second biopsy may be associated with the reproduction of kidney. Less