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腎糸球体の再生過程における糸球体内皮細胞の役割に関する検討

肾小球内皮细胞在肾小球再生过程中的作用研究

基本信息

批准号：
17591103
负责人：
KAWASAKI Yukihiko
金额：
$ 2.35万
依托单位：
Fukushima Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591103/
关键词：
Glomerular endothelial cell nodocvte reproduction FB21 HGF α-SMA ヒト糸球体 HSPN 胎児内皮細胞

项目摘要

To reveal the human glomerulogenesis, we evaluated the process of development of glomeruli and staining for CD31, CD34, FB21, alfa-Smooth Muscle Actin (alfa-SMA), and nephrin in fetal and infant kidneys. We investigated the process of glomerular development in four developmental stages[V-stage, S-stage, C-stage and M-stage]and glomerular CD31, CD34, FB21, alfa-SMA, and Nephrin staining in each group. V-stage proportion was highest in Group A. After 20 weeks, V stage proportion gradually decreased. S- and C-stage proportions were higher in Groups B and C than in Groups D, E and F. M-stage proportion was highest in Group F. CD31, CD34 and FB21 were stained similarly in for endothelial cells in the kidneys of fetuses at over 25 weeks of gestation. Alfa-SMA stained in mesangial cells were found to the lower clefts of the S-shaped bodies first of all. Similarly Nephrin stained in epithelial cells were first observed in S-shaped body weakly. These results suggest that the glomerulus matures … More to M-stage from V stage from 25-34 weeks of gestation and that maturation of endothelial cells starts at 25 weeks and is completed by 35 weeks in the fetus. Epithelial cells and mesangial cells are appeared first at the S-stage.On the other hand, to clarify whether serum concentration of HGF and renal HGF receptor (HGFR) expression can predict the prognosis of renal dysfunction, we evaluated serum concentration of HGF and the expression of HGFR-MET and a -SMA in HSPN patients without crescent formation and with crescent formation. These patients were divided into two groups based on pathological findings on the first biopsy. Group 1 consisted of 20 patients with ISKDC I or II, while group 2 consisted of 35 patients with ISKDC III or VI or V. The mean scores of glomerular and interstitial alpha-SMA staining in group 2 were significantly higher than those in group 1. At second biopsy, the mean scores of c-Met positive staining in group 2 were lower than those in group 1. Our results suggest that the expression of glomerular and interstitial alpha-SMA at first biopsy can predict the prognosis of children with HSPN and the expression of glomerular c-Met at second biopsy may be associated with the reproduction of kidney. Less

为了揭示人类肾小球发生，我们评估了胎儿和婴儿肾脏中肾小球的发育过程以及 CD31、CD34、FB21、α-平滑肌肌动蛋白 (α-SMA) 和去氧肾上腺素的染色。我们研究了肾小球四个发育阶段[V期、S期、C期和M期]的发育过程以及各组肾小球CD31、CD34、FB21、alfa-SMA和Nephrin染色。 A组V期比例最高，20周后V期比例逐渐下降。 B 组和 C 组的 S 期和 C 期比例高于 D、E 和 F 组。F 组的 M 期比例最高。妊娠 25 周以上胎儿肾脏内皮细胞的 CD31、CD34 和 FB21 的染色类似。首先在S形体的下裂处发现了系膜细胞中的Alfa-SMA染色。类似地，上皮细胞中的Nephrin染色首先在S形体中观察到较弱。这些结果表明，肾小球在妊娠 25-34 周从 V 阶段成熟到 M 阶段，内皮细胞的成熟在 25 周开始，并在胎儿 35 周时完成。上皮细胞和系膜细胞在S期首先出现。另一方面，为了阐明血清HGF浓度和肾HGF受体（HGFR）表达是否可以预测肾功能障碍的预后，我们评估了无新月体形成和新月体形成的HSPN患者的血清HGF浓度以及HGFR-MET和a-SMA的表达。根据第一次活检的病理结果将这些患者分为两组。第1组由20名ISKDC I或II患者组成，第2组由35名ISKDC III或VI或V患者组成。第2组肾小球和间质α-SMA染色的平均得分显着高于第1组。第二次活检时，第2组c-Met阳性染色的平均得分低于第1组。我们的结果表明，肾小球和间质α-SMA染色的平均得分低于第1组。首次活检α-SMA可预测HSPN患儿的预后，二次活检肾小球c-Met表达可能与肾脏的再生有关。较少的