The role of glomerular endothelial cell and accelerated regenerative factors in renal injur

本研究观察了HUS小鼠肾脏的恢复过程及其与VEGF、Angiopoietin-1的关系。将C57 BL/6小鼠分为三组。第1组为腹腔注射300 μ g/kg和225 ng/kg Stx 2的LPS组，第2组为腹腔注射100 μ g/kg和100 ng/kg Stx 2的低剂量LPS组。对照组小鼠接种生理盐水。从给药后随时间处死的每组5只小鼠中取出肾脏进行组织学评价。第1组的血清Crs高于第2组，第1组的所有小鼠在72-80小时死亡，第2组的所有小鼠均存活。两组均见红细胞碎片。第1组24小时的内皮损伤和系膜溶解评分高于第2组。另外，第2组肾小球系膜增生和系膜基质评分、CD 31阳性表达在72 h均高于第1组。第2组肾小球VEGF和Angiopoietin-1表达逐渐增加，72 h至7 d达高峰。低剂量LPS和Stx 2诱导的HUS小鼠是研究急性肾损伤恢复过程的理想模型，VEGF和Angiopoietin-1在其恢复过程中起重要作用。

We examined the process of the renal recovery in mice with HUS and the relationship between its renal recovery and VEGF, Angiopoietin-1. C57BL/6 mice were divided into three groups. Group 1 was consisted of mice received an inyraperitoneal injection of dose of LPS at 300 μ g/kg and 225 ng/kg Stx2, Group 2 was consisted of mice received an inyraperitoneal injection of a low dose of LPS at 100 μ g/kg and 100 ng/kg Stx2. Mice in the control group were inoculated with saline. The kidneys were extirpated from 5 mice of each group sacrificed with time after administration for histological evaluation. Serum Crs in Group 1 were higher than those in Group 2 and all mice in Group 1 were died at 72-80 hours and all mice in Group 2 were alive. The fragmented erythrocytes were found in both groups. Endothelial injury and mesangiolysis scores at 24 hours in Group 1 were higher than those in Group 2. In addition, mesangial proliferation and mesangial matrix scores, CD31 positive expression at 72 hours in Group 2 were higher than those in Group 1. Glomerular VEGF and Angiopoietin-1 expressing in Group 2 gradually increased and were peak from 72 hours to 7 days. HUS mice induced by low dose of LPS and Stx2 were useful model for the recovery process of acute renal injury and VEGF and Angiopoietin-1 play important roles of its recovery process.