Efficacy of malignant tumor detection with 0-(2-[^<18>F]fluoroethy1)-L-tyrosine (FET)

0-(2-[^18F]氟乙基1)-L-酪氨酸(FET)检测恶性肿瘤的功效

基本信息

批准号：
17591279
负责人：
ABE Koichiro
金额：
$ 2.27万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The amino acid tracer 0-(2-[^<18> ^F)] fluoroethy1) -L-tyrosine (FET) is a recently developed amino acid analogue, which can be synthesized easily with high radiochemical yield. The purposes of this study were to address the biodistribution of FET and to evaluate the usefulness of FET for the diagnosis of malignancy. C57BL/6 mice were s.c. inoculated with 1x106 EL4 cells in 100μL of PBS (phosphate based saline) into rt. flank (tumor model). Mice were also s.c. administrated 100μL of complete Freund's adjuvant (CFA) into 1t. flank 4 days before and 3 days after tumor inoculation (inflammation model). Seven days after tumor inoculation, mice were i.v. injected with 37-185 MBq FET via tail vein. After injection, mice were sacrificed on several time points. In all normal organs, FET accumulation rapidly increased and peaked within 30 min after the injection and afterward gradually decreased. The highest FET uptake was observed in pancreas and was about 4 times higher than other organs. The uptake of FET in the tumor tissue was significantly higher than other organs and inflammatory lesion. FET was administrated in four normal volunteers. Dynamic analysis showed that FET accumulation in normal organs increased rapidly and peaked within 5 min after the injection. The accumulation in organs were gradually decreased and reached a plateau at 20 min. Static images taken 60 min after FET administration showed that FET uptake in normal organs were nearly equal or slightly higher than that of muscle. This study shows that FET is considered to be a useful amino acid tracer for tumor imaging. It would have the potential to differentiate tumor from inflammation.

氨基酸示踪剂0-（2 - [ ^<18> ^f）]氟乙醇1）-l-酪氨酸（FET）是最近开发的氨基酸类似物，可以轻松地以较高的放射化学产率合成。这项研究的目的是解决FET的生物分布，并评估FET对诊断恶性肿瘤的有用性。 C57BL/6小鼠是S.C.将1x106 EL4细胞接种在100μlPBS（磷酸盐盐水）中的RT中。侧面（肿瘤模型）。小鼠也是S.C.行政100μl完整的Freund调整（CFA）为1T。肿瘤接种前4天和3天（炎症模型）前4天。肿瘤接种后7天，小鼠为静脉注射。通过尾静脉注射37-185 MBQ FET。注射后，小鼠在几个时间点被扫聚。在所有正常器官中，注射后的30分钟内，FET积累迅速增加并达到峰值，然后逐渐改善。在胰腺中观察到最高的FET摄取，比其他器官高约4倍。 FET在肿瘤组织中的摄取明显高于其他器官和炎症性病变。 FET在四名正常志愿者中施用。动态分析表明，在注射后5分钟内，正常器官的FET积累迅速增加，并在峰值上达到峰值。器官的积累逐渐发展，并在20分钟时达到平稳状态。 FET给药60分钟后拍摄的静态图像显示，正常器官的FET摄取几乎等于或略高于肌肉。这项研究表明，FET被认为是用于肿瘤成像的有用的氨基酸示踪剂。它有可能区分肿瘤与炎症。