Efficacy of malignant tumor detection with 0-(2-[^<18>F]fluoroethy1)-L-tyrosine (FET)

0-(2-[^18F]氟乙基1)-L-酪氨酸(FET)检测恶性肿瘤的功效

• 批准号: 17591279
• 负责人: ABE Koichiro
• 金额: $ 2.27万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591279/
• 关键词: Fluorine-labeled amino acid; Fluoroethy1 tyrosine; PET; Malignancy detection; フッ素18標識アミノ酸製剤

The amino acid tracer 0-(2-[^<18> ^F)] fluoroethy1) -L-tyrosine (FET) is a recently developed amino acid analogue, which can be synthesized easily with high radiochemical yield. The purposes of this study were to address the biodistribution of FET and to evaluate the usefulness of FET for the diagnosis of malignancy. C57BL/6 mice were s.c. inoculated with 1x106 EL4 cells in 100μL of PBS (phosphate based saline) into rt. flank (tumor model). Mice were also s.c. administrated 100μL of complete Freund's adjuvant (CFA) into 1t. flank 4 days before and 3 days after tumor inoculation (inflammation model). Seven days after tumor inoculation, mice were i.v. injected with 37-185 MBq FET via tail vein. After injection, mice were sacrificed on several time points. In all normal organs, FET accumulation rapidly increased and peaked within 30 min after the injection and afterward gradually decreased. The highest FET uptake was observed in pancreas and was about 4 times higher than other organs. The uptake of FET in the tumor tissue was significantly higher than other organs and inflammatory lesion. FET was administrated in four normal volunteers. Dynamic analysis showed that FET accumulation in normal organs increased rapidly and peaked within 5 min after the injection. The accumulation in organs were gradually decreased and reached a plateau at 20 min. Static images taken 60 min after FET administration showed that FET uptake in normal organs were nearly equal or slightly higher than that of muscle. This study shows that FET is considered to be a useful amino acid tracer for tumor imaging. It would have the potential to differentiate tumor from inflammation.

氨基酸示踪剂0-（2-[1，2<18>-F]氟乙基）-L-酪氨酸（FET）是近年来发展起来的一种氨基酸类似物，具有合成简便、放化产率高等优点。本研究的目的是解决FET的生物分布，并评估FET诊断恶性肿瘤的有用性。将C57 BL/6小鼠s.c.用溶于100μL PBS（磷酸盐盐水）的1x 106个EL 4细胞接种至右侧腹（肿瘤模型）。小鼠也被s.c.将100μL完全弗氏佐剂（CFA）注入1 t.在肿瘤接种前4天和肿瘤接种后3天侧腹（炎症模型）。肿瘤接种后7天，通过尾静脉给小鼠静脉内注射37-185 MBq FET。注射后，在几个时间点处死小鼠。在所有正常器官中，FET蓄积迅速增加，并在注射后30 min内达到峰值，随后逐渐下降。在胰腺中观察到最高的FET摄取，并且比其他器官高约4倍。肿瘤组织对FET的摄取明显高于其他器官和炎性病变。四名正常志愿者接受FET。动态分析显示，FET在正常器官中的蓄积迅速增加，并在注射后5 min内达到峰值。在器官中的积累逐渐减少，并在20分钟达到一个平台。FET给药后60分钟拍摄的静态图像显示，在正常器官中的FET摄取几乎等于或略高于肌肉。这项研究表明，FET被认为是一种有用的肿瘤成像氨基酸示踪剂。它有可能区分肿瘤和炎症。

项目成果

An analysis of FET biodistribution in tumor-bearing mouse

荷瘤小鼠体内 FET 生物分布分析

• 发表时间: 2005
• 作者: ABE;KOICHIRO
• 通讯作者: KOICHIRO

O-(2-[18F]fluoroethyl)-l-tyrosine (18F-FET) uptake in mouse thymoma cells, and its biodistribution in mice and human volunteers

小鼠胸腺瘤细胞中的 O-(2-[18F]氟乙基)-l-酪氨酸 (18F-FET) 摄取及其在小鼠和人类志愿者中的生物分布

• DOI: 10.1080/02841850600979055
• 发表时间: 2006
• 期刊: Acta Radiologica
• 影响因子: 1.3
• 作者: K. Abe;K. Hayashi;M. Sasaki;H. Koga;K. Kaneko;H. Sawamoto;K. Himuro;H. Honda
• 通讯作者: H. Honda

健常者における18F-FETの体内分布の検討

检查健康受试者中 18F-FET 的生物分布

• 发表时间: 2005
• 作者: K.Abe;K.Hayashi;M.Sasaki;H.Koga;K.Kaneko;H.Sawamoto;K.Himuro;H.Honda;Koichiiro Abe;阿部 光一郎
• 通讯作者: 阿部 光一郎

0-(2-[^<18>F]fluoroethy1)-L-tyrosine(^<18>F-FET) uptake in mouse thymoma cells, and its biodistribution in mice and human volunteers

小鼠胸腺瘤细胞中的0-(2-[^ 18 F]氟乙基1)-L-酪氨酸(^ 18 F-FET)摄取及其在小鼠和人类志愿者中的生物分布

• 发表时间: 2006
• 期刊: ACTA RADIOLOGICA 47.10
• 作者: K.Abe;K.Hayashi;M.Sasaki;H.Koga;K.Kaneko;H.Sawamoto;K.Himuro;H.Honda
• 通讯作者: H.Honda

担癌マウスにおける18F-FET集積の検討

荷瘤小鼠 18F-FET 积累的检查

• 发表时间: 2004
• 作者: ABE;KOICHIRO;阿部光 一郎
• 通讯作者: 阿部光 一郎