Experimental study of reconstruction of peripheral nerve defect using FGF-DDS bioabsorbable nerve conduit

前言：近年来，各种可生物降解的聚合物管材相继报道。然而，这些结果并不能令人满意的临床应用。我们已经调查了我们的明胶填充成纤维细胞生长因子-2的可生物降解神经导管是一种可靠的药物输送系统(DDS)，它可以释放成纤维细胞生长因子-2两周。本研究的目的是介绍我们的新型生物可降解聚合物管填充明胶和成纤维细胞生长因子-2作为DDS，并报告其对周围神经再生和血管生成的影响。材料和方法：研究1：在Wistar大鼠坐骨神经12 mm缺损处植入生物可降解聚合物导管，填充明胶和未结合成纤维细胞生长因子2。植入后12周取材，组织学观察管道中段横断面再生神经和血管的数量。研究二：DDS-2对周围神经再生、血管生成和雪旺细胞迁移的促进作用：在同一系列中，在术后5d、10d、15d、20d，免疫组织学观察神经再生与雪旺细胞和血管在纵切面近端残端的关系。结果：研究1：对于成纤维细胞生长因子-2，12周的神经再生结果明显优于DDS和成纤维细胞生长因子-2。研究二：FGF2的DDS在15d和20d能促进神经再生，雪旺细胞在20d内迁移，在15d和20d有明显的血管生成促进作用。

Introduction: Recently, various biodegrable polymer tubes have been reported. However those results were not satisfying enough for clinical use.We have already investigated that our biodegradable nerve conduit filled with gelatin incorporating FGF-2 is a reliable drug delivery system (DDS) and it could release FGF-2 for two weeks.The purpose of this study is to introduce our novel biodegradable polymer tube filled with gelatin incorporating FGF-2 as DDS and to report its effect on peripheral nerve regeneration and angiogenesis.Materials and Methods: Study #1; effects of DDS of FGF-2 on peripheral nerve regeneration, Schwann cell and angiogenesis :we implanted biodegradable polymer conduit filled gelatin with or without incorporating FGF-2 into 12 mm sciatic nerve gaps in Wistar rats. At 12 weeks after implantation, number of regenerating nerves and vessels in transverse sections at mid-conduit were observed histologically. Study #2; enhancement of DDS FGF-2 on peripheral nerve regeneration, angiogenesis and Schwann cell migration:In the same series, at 5D, 10D, 15D, 20D post operation, relationship of regenerating nerves, Schwann cells and vessels from proximal stump in longitudinal sections were observed immuno-histologically.Results: Study #1; Concerning FGF-2, the results of nerve regeneration at 12 weeks obviously showed the advantage of DDS with FGF-2. And the number of vessels and total area of vessels in conduit significantly increased with FGF-2, too.Study #2; DDS of FGF-2 could enhance nerve regeneration at 15D and 20D, Schwann cell migration throughout 20 days, angiogenesis at 15D and 20D.Among them, angiogenesis was obviously enhanced.