Experimental study of reconstruction of peripheral nerve defect using FGF-DDS bioabsorbable nerve conduit

FGF-DDS生物可吸收神经导管重建周围神经缺损的实验研究

• 批准号: 17591587
• 负责人: TAKAMATSU Kiyohito
• 金额: $ 1.93万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591587/
• 关键词: peripheral nerve; nerve conduit; nerve regeneration; tissue engineering; bioabsorbable material; drug delivery system; nerve growth factor; 成長因子; 移植再生医療; 生体材料; 再生医学; 脳神経

Introduction: Recently, various biodegrable polymer tubes have been reported. However those results were not satisfying enough for clinical use.We have already investigated that our biodegradable nerve conduit filled with gelatin incorporating FGF-2 is a reliable drug delivery system (DDS) and it could release FGF-2 for two weeks.The purpose of this study is to introduce our novel biodegradable polymer tube filled with gelatin incorporating FGF-2 as DDS and to report its effect on peripheral nerve regeneration and angiogenesis.Materials and Methods: Study #1; effects of DDS of FGF-2 on peripheral nerve regeneration, Schwann cell and angiogenesis :we implanted biodegradable polymer conduit filled gelatin with or without incorporating FGF-2 into 12 mm sciatic nerve gaps in Wistar rats. At 12 weeks after implantation, number of regenerating nerves and vessels in transverse sections at mid-conduit were observed histologically. Study #2; enhancement of DDS FGF-2 on peripheral nerve regeneration, angiogenesis and Schwann cell migration:In the same series, at 5D, 10D, 15D, 20D post operation, relationship of regenerating nerves, Schwann cells and vessels from proximal stump in longitudinal sections were observed immuno-histologically.Results: Study #1; Concerning FGF-2, the results of nerve regeneration at 12 weeks obviously showed the advantage of DDS with FGF-2. And the number of vessels and total area of vessels in conduit significantly increased with FGF-2, too.Study #2; DDS of FGF-2 could enhance nerve regeneration at 15D and 20D, Schwann cell migration throughout 20 days, angiogenesis at 15D and 20D.Among them, angiogenesis was obviously enhanced.

简介：最近，各种生物降解聚合物管已被报道。本研究的目的是介绍一种新型的可生物降解的明胶-FGF-2复合神经导管，该导管具有良好的生物相容性和生物相容性。材料和方法：研究#1; FGF-2的DDS对周围神经再生、雪旺细胞和血管生成的影响：我们将含有或不含有FGF-2的填充明胶的可生物降解聚合物导管植入Wistar大鼠的12 mm坐骨神经间隙中。植入后12周，组织学观察导管中段横切面再生神经和血管的数量。研究2; DDS FGF-2对周围神经再生、血管生成和雪旺细胞迁移的促进作用：在同一组动物中，于术后5D、10 D、15 D、20 D，在纵断面上观察再生神经、雪旺细胞和近端血管的关系。研究2：FGF-2的DDS在15 d和20 d时可促进神经再生，在20 d时可促进雪旺细胞迁移，在15 d和20 d时可促进血管新生，其中血管新生明显增强。

项目成果

Biodegradable nerve conduit with FGF2-DDS could enhance peripheral nerve regeneration,Schwann cell migration and angiogenesis

含有 FGF2-DDS 的可生物降解神经导管可以增强周围神经再生、雪旺细胞迁移和血管生成

• 发表时间: 2007
• 作者: 藤間保晶;田所美香;大串始;森下亨;土肥祥子;田中康仁;朴木寛弥;高倉義典;高松 聖仁
• 通讯作者: 高松 聖仁