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The study on new artificial red blood cells (encapsulation Hb) at the shock

新型人工红细胞（封装Hb）冲击时的研究

基本信息

批准号：
17591886
负责人：
IKEGAWA Hitoshi
金额：
$ 2.5万
依托单位：
Osaka university
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591886/
关键词：
liposome-encapsulated hemoglobin tissue oxygen metabolism oxygen delivery 酸素運搬能

项目摘要

The present study was performed to clarify the effect of blood exchange with liposome-encapsulated hemoglobin (LEH) on oxygen metabolism. Fifteen New Zealand White rabbits were anesthetized with pentobarbital sodium and randomly divided into three groups (n=5, each) based on the materials of blood substitute : LEH group with LEH suspension ([Hb]=6g/dL in saline containing 3% alubumin), RBC group with shed blood diluted 1:1 with saline containing 3% alubumin, and Plasma group with saline containing 3% alubumin without hemoglobin. After baseline measurements, 9mL/kg of circulating blood was withdrawn in 150sec and the identical amount of blood substitute was subsequently infused in 150sec. This procedure was repeated eight times in 5-min cycles for 40min to achieve blood exchange at approximately 90% of total circulating blood. Hemodynamics and oxygen metabolism parameters were measured at 60, 90, 120min after the beginning of blood exchange. Rabbits were subjected to stepwise cardiac tamponade to reduce oxygen delivery (DO_2) thereafter. The oxygen consumption (VO_2)/DO_2 relation was analyzed by the dual line method. Mean values of [Hb] after blood exchange in groups LEH, RBC, and Plasma were 5.7g/dL, 6.0g/dL, and 1.2g/dL, respectively. Mean arterial pressure, heart rate, and cardiac output in groups LEH and RBC at 60, 90, and 120min did not significantly differ from the corresponding baseline values. All animals in Plasma group died by 120min due to circulatory shock. After blood exchange, DO_2 values for groups LEH and RBC were reduced by approximately 50%, but VO_2 values remained consistent in comparison with the baseline values. In the VO_2/DO_2 relation, the slope of the supply-dependent line for LEH group (y=0.69x+1.8) was not significantly different from that for RBC group (y=0.68x+2.3). These results indicate that LEH is a potent blood substitute with respect to the ability of tissues to extract oxygen even in case of massive transfusion.

本研究旨在阐明脂质体包裹血红蛋白（莱赫）血液交换对氧代谢的影响。新西兰白色兔15只，戊巴比妥钠麻醉后，根据血液代用品的材料随机分为3组（n=5）：莱赫组（含莱赫混悬液，[Hb]= 6 g/dL）、RBC组（含3%白蛋白的生理盐水1：1稀释）、血浆组（含3%白蛋白的生理盐水不含血红蛋白）。基线测量后，在150秒内抽取9 mL/kg循环血液，随后在150秒内输注等量血液代用品。该程序以5分钟为周期重复8次，持续40分钟，以实现约90%的总循环血液的血液交换。分别于血液交换开始后60、90、120 min测定血流动力学和氧代谢指标。随后对家兔进行逐步心脏压塞以减少氧输送（DO_2）。用双线法分析耗氧量（VO_2）/DO_2关系。莱赫组、红细胞组和血浆组换血后[Hb]的平均值分别为5.7 g/dL、6.0 g/dL和1.2 g/dL。莱赫组和RBC组在60、90和120分钟时的平均动脉压、心率和心输出量与相应的基线值无显著差异。血浆组120 min内全部动物死于循环性休克。血液交换后，莱赫组和RBC组的DO_2值降低约50%，但VO_2值与基线值相比保持一致。在VO 2/DO 2关系中，莱赫组的供应依赖线斜率（y=0.69x+1.8）与红细胞组的供应依赖线斜率（y=0.68x+2.3）无显著差异。这些结果表明，即使在大量输血的情况下，就组织提取氧气的能力而言，莱赫是一种有效的血液替代品。