Elastic System Fibers Formation and Degradation in the Periodontal Tissues

牙周组织中弹性系统纤维的形成和降解

基本信息

批准号：
17591920
负责人：
YAJIMA Toshihiko
金额：
$ 2.39万
依托单位：
Health Sciences University of Hokkaido
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591920/
关键词：
elastic system fibers microfibrils elastin fibrillin MAGP-1 MMP-2 periodontal tissues fibroblasts フィブリリン-2 MMP inhibitor TIMP-2 フィビユリン-5 フィブリン

项目摘要

Elastic system fibers, comprised of microfibrils and tropoelastin, are extracellular components of periodontal tissue. Elastogenesis requires a microfibril template onto which tropoelastin is deposited. The microfibrils, which are made up mainly of fibrillins, microfibril-associated glycoporoteins (MAGPs) and fibulins, are thought to serve as a template structure that guides tropoelastin desposition.In this study, we used the RNA interference (RNAi) technique to estanblish MAGP-1 and fibrillin-2 gene-specific knock-downs individually in human gingival fibroblasts (HGF). We then examined the extracellular deposition of tropoelastin by western blotting. These two genes were specifically suppressed to <30% of the control level, and this was responsible for the diminution of tropoelastin deposition. An immunof;uorescence study also cinfrimed that RNAi-mediated down-regulation of MAGP-1 or fibrillin-2 led to the loss of tropoelastin immunoreactivity. These results suggest that MAGP-1 and fibrillin-2 are associated with the extracellular deposition of tropoelastin during elastic fiber formation in HGF in vitro.The process of elastic system fibersr remodeling is not fully understood. We examined whether matrix metaroproteinases (MMPs) are involved in remodeling of fibrillins by HGF and human periodontal ligament fibroblasts (HPLF). Active MMP-2 appeared as cell-membrane-associated or in extracellular matrix only in HPDF cell layers. The addition of MMP inhibitor or tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) significantly increased fibrillin-2 accumulation in HPDF cell layers, suggesting that active MMP-2 may degrade fibrillin-2, and that MMPs may play a role in the remodeling of elastic system fibers in periodontal ligament.

弹性系统纤维由微纤维和热带蛋白酶组成，是牙周组织的细胞外成分。弹性生成需要一个微纤维模板，将肌动蛋白沉积在该模板上。微纤维主要由原纤维蛋白，微纤维相关的糖胶蛋白（MAGP）和纤维蛋白组成，被认为是指导Tropoelastin distosition的模板结构。成纤维细胞（HGF）。然后，我们通过蛋白质印迹检查了tropoelastin的细胞外沉积。这两个基因被特异性抑制至对照水平的30％，这是造成心poelastin沉积减少的原因。一项免疫;杀菌研究还表明，RNAi介导的MAGP-1或Fibrillin-2的下调导致了肌动蛋白免疫反应性的丧失。这些结果表明，在HGF体外，弹性纤维形成期间，Magp-1和Fibrillin-2与弹性纤维形成期间的细胞外沉积有关。我们检查了基质元蛋白酶（MMP）是否参与了HGF和人类牙周韧带成纤维细胞（HPLF）对原纤维进行重塑。活性MMP-2仅在HPDF细胞层中以细胞膜相关或细胞外基质的形式出现。基质金属蛋白酶-2（TIMP-2）的MMP抑制剂或组织抑制剂的添加显着增加了HPDF细胞层中的纤维蛋白-2积累，这表明主动MMP-2可能降解纤维蛋白-2，并且MMP可能在周期内弹性系统纤维的重塑中起作用。