Synthetic Studies of Age Changes on Priodontium

原齿菌年龄变化的综合研究

• 批准号: 08307015
• 负责人: YAJIMA Toshihiko
• 金额: $ 11.78万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08307015/
• 关键词: Periodontium; Age changes; Periodontal fibroblasts; Cell cycle; Proliferative capacity; Interleukin production; PGE; Collagen secretion and degradation; c-fos発現; IL-6産生; コラーゲン産生; 歯周靭帯細胞; 歯肉線維芽細胞; アルカリ性ホスファターゼ; PGE_2産生; カテプシンB,L

The periodntium contributes to the maintenance of a healthy functional supporting system for teeth. These tissues are affected various effects of aging, and appear functional and organic deficiencies. The aim of this project, therefore, was to determine the age changes on cell activities and tissue functions of the periodontium.1. We compared the in vitro prolifreative capacity of human periodontal ligament (HPL) cells from aged and juvenile donors. There were no differences in the length of each phase of the cell cycle ; significant differences were found in the ratio of the cels entering from G1 to DNA synthesis phase of the cell cycle. Replicative capacity was much longer in juvenile donors, while aged donors showed short dividing abilities. Additionally, no c-fos was detected in cells which had reached senescence upon stimulation with serum.2. We investigated the effects of in vitro cellular aging on alkaline phosphatase (ALPase), cathesin activities and collagen secretion from HPL … More cells. ALPase activity and collagen secretion were found to have significantly decreased while cathepsin B and L activities significantly increased with cellular aging.3. We examined the effects of in vitro cellular aging with mechanical stress on IL-1beta protein and expression by HPL cells. A two-fold increase in IL-1beta production in response to mechanical stress was found old cells compared with that of young cells, although the constitutive levels of IL-1beta were similar in both cells. IL-1beta mRNA was detected in both the cells types under basal conditions, and its expression was further enhanced by application of mechanical tension.4. We investigated the effect of aging on responses of human gingival fibroblasts (HGF) to LPS from P.gingivalis and Campylobacter rectus. Levels of IL-6 per DNA decreased in HGF from young patients, but increased in the HGF from old patients according to increase of the LPS concentration. The LPS-stimulated PGE_2 production in old cells was significantly increased than that in the corresponding young cells.The results suggested that aged tissues are characterized by a decreased turnover rate and repressed resistance to tissue destruction and, hence, a low wound healing ability. It is possible that a large amount of IL-1beta, IL-6 and PGE_2 produced by periodontal cells from an aged host in response to mechanical force and LPS may be positively related to the accretion of alveolar bone resorption. Less

该周期有助于维持健康的牙齿功能支持系统。这些时机受到衰老的各种影响，并显得功能性和有机缺陷。因此，该项目的目的是确定牙周活动的细胞活动和组织功能的年龄变化1。我们比较了老年和少年供体的人牙周韧带（HPL）细胞的体外增生能力。细胞周期的每个阶段的长度没有差异。在细胞周期的从G1到DNA合成阶段的CEL的比率上发现了显着差异。少年捐助者的复制能力更长，而老年捐助者则表现出短暂的分裂能力。另外，在血清刺激后达到感受的细胞中未检测到C-Fos.2。我们研究了体外细胞衰老对HPL…更多细胞中葡萄球菌磷酸酶（ALPase），Cathesin活性和胶原蛋白分泌的影响。发现ALPase活性和胶原蛋白分泌显着降低，而组织蛋白酶B和L活性随细胞衰老而显着增加。3。我们检查了具有机械应力的体外细胞衰老对IL-1BETA蛋白和HPL细胞表达的影响。与年轻细胞相比，发现旧细胞的IL-1BETA产生增加了两倍，尽管在两个细胞中IL-1BETA的组成型水平相似。在基本条件下，在两种细胞类型中都检测到IL-1Beta mRNA，并且通过应用机械张力进一步增强其表达。4。我们研究了衰老对人类牙龈成纤维细胞（HGF）对LPS的反应的影响。年轻患者的HGF中，HGF的IL-6水平降低，但根据LPS浓度的升高，老年患者的HGF水平降低。旧细胞中LPS刺激的PGE_2的产生显着增加了比相应的幼小细胞中的显着增加。结果表明，老化的组织的特征是周转率降低，并且反映了对组织破坏的耐药性，因此，伤口愈合能力低。可能与机械力和LPS相关的牙周细胞产生的大量IL-1BETA，IL-6和PGE_2可能与肺泡骨骼分辨率的积聚成正相关。较少的

项目成果

F.Nishimura et al: "Glucose-mediated alteration of cellular function in human periodontal ligament cells" Journal of Dental Research. 75. 1664-1671 (1996)

F.Nishimura 等人：“人类牙周膜细胞中葡萄糖介导的细胞功能改变”《牙科研究杂志》。

T.Goseki et al.: "Effects of in vitro cellular aging on alkaline phosphatase,cathepsin activities and collagen secretion of human periodontalligament derived cell." Mechanisms of Ageing and Development. 91. 171-183 (1996)

T.Goseki 等人：“体外细胞老化对人牙周膜衍生细胞的碱性磷酸酶、组织蛋白酶活性和胶原蛋白分泌的影响”。

F.Nishimura et al.: "Comparison of in vitro proliferative capacity of human periodontal ligament cells juvenile and aged donors" Oral Desease. 3. 162-166 (1997)

F.Nishimura 等人：“青少年和老年供体牙周膜细胞体外增殖能力的比较”口腔疾病。

M.Muro et al: "Role of CD14 molecules in internalization of Actinobacillus actinomycetemcomitans by macrophages and subsequent induction of apoptosis" Infection and Immunity. 65. 1147-1151 (1997)

M.Muro 等人：“CD14 分子在巨噬细胞内化伴放线放线杆菌并随后诱导细胞凋亡中的作用”感染和免疫。

F.Nishimuraura et al: "Comparision of in vitro proliferative capacity of human periodontal ligament cells in juvenile and aged donors" Oral Diseases. 3. 162-166 (1997)

F.Nishimuraura 等人：“青少年和老年供者牙周膜细胞体外增殖能力的比较”口腔疾病。