Establishment of synvoial cells and analysis of interactions between the cells from synovial tissue

滑膜细胞的建立和滑膜组织细胞之间相互作用的分析

基本信息

批准号：
17592111
负责人：
OGURA Naomi
金额：
$ 2.37万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17592111/
关键词：
temporomandibular joint synovial fibroblasts IL-1β GeneChip analysis chemokines COX2 IL-6 MCP-1 顎関節由来滑膜細胞 Microarray TNF-α 株化テロメアーゼ遺伝子

项目摘要

Interleukin (IL)-1β is a key mediator of intracapsular pathologic conditions of the temporomandibular joint (TMJ), such as internal derangement (ID) and osteoarthritis. Gene expression profile of synovial fibroblasts stimulated with IL-1β was studied by oligonucleotide microarray analysis to elucidate candidate genes associated with intracapsular pathologic conditions of TMJ. Human synovial tissue was obtained from five patients with ID who underwent arthroscopy of the TMJ. The patients gave complete informed consent for the surgery and for the use of their tissue in research. RNA was isolated from synovial fibroblasts from five patients after IL-1β treatment. Gene expression profiling was performed with a GeneChip. Changes in gene expression were determined by comparing IL-1β-treated cells with untreated cells. Gene expressions of IL-8, MCP-1, COX2, IL-1β, IL-6, and M-CSF were increased in synovial fibroblasts treated with IL-1β. Furthermore, we examined that IL-1β was injected into the cavity of the rat TMJ. Twenty-four hours after a single intraarticular injection of IL-1β in rat TMJs, the TMJs showed synovitis-like changes such as an increase in the thickness of the synovial lining, vascular dilation, and infiltration of mononuclear cells in the subsynovial connective tissue. However, the synovial membranes of control TMJs (injected with saline solution) showed non-inflammatory changes. In immunohistochemistry, positive cells of MCP-1, IL-6, and COX2 were detected in the lining and the sublining cells of the synovial tissue of experimental rat TMJs. No positive cells appeared in the control. These data should provided useful information about the pathologic conditions of TMJ, especially in support of diagnosis and therapeutic approaches to TMJ. These experiments were performed according to the guidelines established by the Institutional Review Board (ECO3-003) and the Guidelines for Animal Care (05-003) of Nihon University School of Dentistry at Matsudo.

白介素（IL）-1β是颞下颌关节（TMJ）（例如内进化（ID）和骨关节炎）的关键介体。通过寡核苷酸微阵列分析研究了用IL-1β刺激的滑膜成纤维细胞的基因表达谱，以阐明与TMJ的囊内病理条件相关的候选基因。人类滑膜组织是从五名患有TMJ关节镜检查的ID患者那里获得的。患者在研究中给予了完全知情的手术和使用组织的同意。 IL-1β治疗后，从五名患者的滑膜成纤维细胞中分离RNA。用转基因进行基因表达分析。通过将IL-1β处理的细胞与未处理的细胞进行比较，确定基因表达的变化。 IL-8，MCP-1，COX2，IL-1β，IL-6和M-CSF的基因表达在用IL-1β处理的滑膜成纤维细胞中增加了。此外，我们检查了IL-1β被注入大鼠TMJ的腔。单次关节内注射IL-1β在大鼠TMJ中的二十四小时，TMJS显示出类似滑膜炎的变化，例如滑膜内膜厚度的增加，血管词典的厚度，以及均匀连接组织中单核细胞的浸润。但是，对照TMJ的滑膜（注射盐水溶液）显示出非炎症的变化。在免疫组织化学中，在实验大鼠TMJS的滑膜组织的衬里和升华细胞中检测到MCP-1，IL-6和COX2的阳性细胞。对照中没有阳性细胞出现。这些数据应提供有关TMJ病理状况的有用信息，尤其是在支持TMJ的诊断和治疗方法方面。这些实验是根据Matsudo的Nihon University Dentistry的Nihon University School of Institutional Review委员会（ECO3-003）制定的指南（ECO3-003）制定的。