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Effect of rinsing by sugar solution on oral malodor of tube fad patients

糖液漱口对管型FA患者口腔异味的影响

基本信息

批准号：
17592188
负责人：
KISHI Mitsuo
金额：
$ 2.36万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17592188/
关键词：
Volatile sulfur comnounds (VSC)Hydrogen sulfide (H_2S)Oral malodor β C-S lyase Glucose Tube feeding Sorbitol Saliva 舌苔メチルメルカプタン Streptococcus anginosus 舌清掃プロフェッショナルケア βC-Sリアーゼ Porpyromonas gingivalis 揮発性硫化物経管栄養者ブドウ糖

项目摘要

1. Healthy young adults were ordered to rinse their mouth with 10% glucose solution. Before and after rinsing, VSC in mouth air were determined. Consequently, in rinsing with glucose solution, VSC concentrations was significantly lower than that in the control.Saliva samples from same subjects were incubated for 90min at 37℃ under anaerobic condition with 2% glucose or sorbitol. After the incubation, hydrogen sulfide (H_2S) production was measured. Consequently, the amounts of H2S from saliva with glucose were significantly lower than the control. Furthermore, we determined the cell number of total bacteria, T, forsythia and Fusobacterium in the saliva samples using real-time PCR method. The proportion of T forsythia against total bacteria was constant in all conditions (glucose or sorbitol added and control). From those results, sugar supply was supposed to be effective for reduction of VSC in mouth air. Further, it was suggested that the phenomenon occurred by not only inactivation of anaerobic microbe under low pH condition by sugar fermentation.2. We purified H2S producting enzyme from Streptococcus anginosus. The activities were determined with and without glucose. Consequently, there was no effect of glucose on the activities. It was suggested that the glucose did not effect on the enzyme.3. We determined VSC in mouth air of elderly with and without tube feeding in a nursing institution. The two groups were matched by the oral conditions. Consequently, there was no significant difference in VSC concentration. However, the high correlations between VSC levels and oral malconditions in the elderly with tube feeding. From the results, it was supposed that tube feeding was not causative for oral malodor in case the whole oral health control was successful, but if the some oral conditions were poor, VSC levels got higher especially in the subjects with tube feeding.

1.要求健康的青壮年用10%的葡萄糖溶液漱口。漱口前后分别测定口腔空气中的VSC。因此，在用葡萄糖溶液冲洗时，血管干细胞浓度显著低于对照组。同一受试者的莎草样品在37℃的厌氧条件下，加入2%葡萄糖或山梨醇培养90min。培养结束后，测定硫化氢(H_2S)的生成量。因此，含有葡萄糖的唾液中的硫化氢含量显著低于对照组。采用实时荧光定量聚合酶链式反应检测唾液样品中细菌总数、T、连翘属和梭杆菌的数量。在所有条件下(添加葡萄糖或山梨醇和对照)，T连翘占细菌总数的比例都是恒定的。由此可见，补充糖对降低口腔空气中的VSC是有效的。进一步认为，这一现象不仅是由于糖发酵使厌氧微生物在低pH条件下失活所致。我们从血管紧张链球菌中提纯了硫化氢产生酶。分别在有无葡萄糖的情况下测定其活性。因此，葡萄糖对酶活性没有影响。说明葡萄糖对该酶没有影响。我们测定了护理机构中有无管饲老年人口腔空气中的VSC。两组患者的口腔条件相匹配。因此，VSC浓度没有显著差异。然而，管饲老年患者的VSC水平与口腔疾病的相关性较高。由此可见，在口腔整体卫生控制成功的情况下，管饲不会引起口腔异味，但当某些口腔条件较差时，VSC水平升高，尤其是管饲的受试者。