Exvivo ESR Spin Rrobe Investigation of Stratum Corneum Lipids

角质层脂质的 Exvivo ESR Spin Rrobe 研究

• 批准号: 18500347
• 负责人: NAKAGAWA Kouichi
• 金额: $ 1.46万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500347/
• 关键词: In vivo measurements; stratum corneum lipid; Electron spin resonance; Spin probe; Simulation; Skin; Sebum secretions; ESR

Electron spin resonance (ESR) is useful for studying stratum corneum (SC)lipid ordering. Order parameter obtained from ESR spectra can be a good index for evaluating the fluidity of SC lipids. However, the method for evaluation of ex vivo SC lipids has not been established. We investigated the details of the ESR methods. A glass plate using a cyanoacrylate resin was used to strip the SC lipids. A single-chain aliphatic spin probe, 5-Doxylstearic acid (5-DSA), was used. The SC samples were incubated in the 0.001% 5-DSA in a 0.1% ethanolic aqueous solution for 60 min at 37℃. After the incubation, an ESR glass holder with the incubated SC sample was mounted in the ESR cavity. The newly designed ESR glass holder improved sample preparations as well as ESR measurements. ESR spectra of the SC samples were fully analyzed using a slow-tumbling simulation. The order parameter obtained by the simulation showed depth dependence of SC lipids as a function of depth in the SC. Thus, we established sample preparations of SC lipids and precise ESR measurements and spectral analyses. In addition, we found depth dependence of the ex vivo SC lipids ordering for the first time.

电子自旋共振（ESR）是研究角质层（SC）脂质有序性的有效方法。由ESR谱得到的有序参数可以作为评价SC脂质流动性的一个很好的指标。然而，尚未建立评价离体SC脂质的方法。我们研究了ESR方法的细节。使用氰基丙烯酸酯树脂的玻璃板剥离SC脂质。使用了单链脂肪族自旋探针5-D-硬脂酸（5-DSA）。将SC样品在37℃下在0.1%乙醇水溶液中的0.001% 5-DSA中孵育60 min。孵育后，将装有孵育SC样品的ESR玻璃保持器安装在ESR腔中。新设计的ESR玻璃保持器改进了样品制备和ESR测量。使用慢速翻滚模拟对SC样品的ESR谱进行了全面分析。通过模拟得到的序参数显示SC脂质作为SC中的深度的函数的深度依赖性。因此，我们建立了SC脂质的样品制备和精确的ESR测量和光谱分析。此外，我们首次发现了离体SC脂质排序的深度依赖性。

项目成果

Pulsed EPR study of low-dose irradiation effects on L-alanine crystal irradiated with γ-rays,Ne,and Si ion beams

γ射线、Ne、Si离子束低剂量辐照对L-丙氨酸晶体的脉冲EPR研究

• 发表时间: 2007
• 期刊: Radiat.Meas. 42
• 作者: B.Rakvin;N.Maltar-Strmeck;and K.Nakagawa
• 通讯作者: and K.Nakagawa

Spin-Probe Investigatin of a Head Group Behavior in Aqueous Dispersion of a Nanionic Amphiphilic Compound

自旋探针研究纳米离子两亲性化合物水分散体中头基行为

• 发表时间: 2007
• 期刊: Lipids (in press)
• 作者: Kokuryo D;Kumamoto E;Okada A;Murakami T;Fujii S;KaiharaT;Kuroda K;K.Nakagawa
• 通讯作者: K.Nakagawa

Analysis of Ex-Vivo Stratum Corneum Lipid Ordering by A Slow-Tumbling Simulation for Electron Paramagnetic Resonance

通过电子顺磁共振慢翻滚模拟分析离体角质层脂质排序

• 发表时间: 2007
• 作者: 黒田輝;小原真;Cauteren MV.;K.Nakagawa
• 通讯作者: K.Nakagawa

ESRスピンプローブ法による皮膚角層脂質の配列構造の研究

ESR自旋探针法研究皮肤角质层脂质排列结构

• 发表时间: 2007
• 作者: 中川 公一;安西 和紀;坂本 一民
• 通讯作者: 坂本 一民

Spin-Probe Investigation of a Head Group Behavior in Aqueous Dispersion of a Nonionic Amphiphilic Compound

非离子两亲化合物水分散体中头基行为的自旋探针研究

• 发表时间: 2007
• 期刊: Lipids 42
• 作者: K. Kobayashi;A. Kisi;M. Tanaka;H. Inaoka;S. Nebuya;Y. Fukuoka;H. Kobayashi;M. Noshiro;K.Nakagawa
• 通讯作者: K.Nakagawa