Impact of different Ceramides to Structure of Stratum corneum lipid model membranes

不同神经酰胺对角质层脂质模型膜结构的影响

• 批准号: 111185811
• 负责人: Professor Dr. Reinhard Neubert
• 金额: --
• 依托单位: Abteilung Biochemische Pharmazie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/111185811?language=en
• 关键词: Impact; different; Ceramides; Structure; Stratum

Quaternary systems composed of defined Stratum corneum (SC) - lipids will be prepared as oriented multilamellar membranes on a quartz surface. The influence of different SC lipid species with focus on the ceramides to the nanostructure and the properties of these membranes will be investigated using neutron diffraction. The main advantage of neutron diffraction is the use specifically deuterated ceramides for the investigation as the position of those deuterated molecular groups are easily detectable within the SC lipid model membranes.In this project the influence of different SC lipids – especially the role of the most hydrophilic ceramide [AP] and of the most lipophilic ceramide [EOS] with the very long N-acetyl tail will be investigated in order to confirm the armature reinforcement model. Therefore, specifically deuterated ceramide [AP] and ceramide [EOS] are to be synthesized in this project. Furthermore, the impact of ceramide [AS] on the nanostructure SC lipid model membranes is to be studied.This project will be carried out through the collaboration between chemists, pharmacists as well as through the cooperation with physicists from the Frank Laboratory of Neutron Physics.

将由确定的角质层（SC）-脂质组成的四元系统制备为石英表面上的定向多层膜。不同的SC脂质物种的影响，重点是神经酰胺的纳米结构和这些膜的性能将使用中子衍射进行研究。中子衍射的主要优点是特别使用氘代神经酰胺进行研究，因为这些氘代分子基团的位置在SC脂质模型膜中很容易检测。在该项目中，不同SC脂质的影响-特别是最亲水的神经酰胺[AP]和具有非常长的N-末端的最亲脂的神经酰胺[EOS]的作用。将对乙酰尾部进行研究，以确认电枢增强模型。因此，在本项目中，将具体合成氘代神经酰胺[AP]和神经酰胺[EOS]。此外，神经酰胺[AS]对纳米结构SC脂质模型膜的影响有待研究。该项目将通过化学家，药剂师之间的合作以及通过与中子物理弗兰克实验室的物理学家的合作进行。

项目成果

Characterisation of a new ceramide EOS species: synthesis and investigation of the thermotropic phase behaviour and influence on the bilayer architecture of stratum corneum lipid model membranes

• DOI: 10.1039/c1sm05134b
• 发表时间: 2011-09
• 期刊: Soft Matter
• 影响因子: 3.4
• 作者: T. Engelbrecht;T. Hauß;Kevin Süβ;Alexander Vogel;Matthew Roark;S. Feller;R. Neubert;B. Dobner

Basic nanostructure of stratum corneum lipid matrices based on ceramides [EOS] and [AP]: a neutron diffraction study.

基于神经酰胺 [EOS] 和 [AP] 的角质层脂质基质的基本纳米结构：中子衍射研究

• DOI: 10.1016/j.bpj.2009.05.041
• 发表时间: 2009
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Schröter;Kessner;Kiselev;Neubert
• 通讯作者: Neubert

Study of the Influence of the Penetration Enhancer Isopropyl Myristate on the Nanostructure of Stratum Corneum Lipid Model Membranes Using Neutron Diffraction and Deuterium Labelling

• DOI: 10.1159/000338538
• 发表时间: 2012-01-01
• 期刊: SKIN PHARMACOLOGY AND PHYSIOLOGY
• 影响因子: 2.7
• 作者: Engelbrecht, T. N.;Deme, B.;Neubert, R. H. H.
• 通讯作者: Neubert, R. H. H.