Impact of different Ceramides to Structure of Stratum corneum lipid model membranes

由定义的角质层(SC)-脂类组成的四元体系将在石英表面上制备成定向多层膜。用中子衍射法研究了不同的SC类脂物种对神经酰胺纳米结构和性能的影响。中子衍射法的主要优点是使用特定的氚神经酰胺进行研究，因为在SC脂质模型膜中可以很容易地检测到这些氚分子基团的位置。在本项目中，将研究不同SC脂类的影响，特别是最亲水的神经酰胺[AP]和最亲油的神经酰胺[EOS]的作用，以证实电枢增强模型。因此，在本项目中，我们要合成的是专门的氢化神经酰胺[AP]和神经酰胺[EOS]。此外，还将研究神经酰胺[As]对纳米结构SC脂质模型膜的影响。该项目将通过化学家、药剂师以及弗兰克中子物理实验室的物理学家的合作来进行。

Quaternary systems composed of defined Stratum corneum (SC) - lipids will be prepared as oriented multilamellar membranes on a quartz surface. The influence of different SC lipid species with focus on the ceramides to the nanostructure and the properties of these membranes will be investigated using neutron diffraction. The main advantage of neutron diffraction is the use specifically deuterated ceramides for the investigation as the position of those deuterated molecular groups are easily detectable within the SC lipid model membranes.In this project the influence of different SC lipids – especially the role of the most hydrophilic ceramide [AP] and of the most lipophilic ceramide [EOS] with the very long N-acetyl tail will be investigated in order to confirm the armature reinforcement model. Therefore, specifically deuterated ceramide [AP] and ceramide [EOS] are to be synthesized in this project. Furthermore, the impact of ceramide [AS] on the nanostructure SC lipid model membranes is to be studied.This project will be carried out through the collaboration between chemists, pharmacists as well as through the cooperation with physicists from the Frank Laboratory of Neutron Physics.