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Effect of exercise training on salivary gland through parasympathetic nervous system

运动训练通过副交感神经系统对唾液腺的影响

基本信息

批准号：
18500516
负责人：
YOSHINO Yoko
金额：
$ 1.03万
依托单位：
Kamakura Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Objective : The objective of the present study was to examine the effect of exercise training on salivary gland through parasympathetic nervous system.Materials and methods: Male ICR mice, weighing 39〜50g, at 32 weeks of age were divided into three groups; an exercise with food restriction (EXP), an exercise without food restriction (EXA), and a control (CTL). EXP group uses voluntary running wheel for exercise, and fed the same amount of diet as the CTL group (pair-feeding). The pilocarpine stimulated whole saliva was collected from the oral cavity by micro-pipet over 15 min 4, 8, and 12 weeks after beginning of the exercise with pair-feeding. The salivary flow rate, protein concentration, and amylase and kallikrein activities were determined. Kallikrein and amylase release are evoked by alpha- and beta- adrenergic receptor stimulation, respectively.Results: The body weight of EXP mice was approximately 20% less than that of CTL mice between 4 and 12 weeks after the beginning of the experiment (p<0.05). Salivary 8-OHdG was significantly increased in the EXP group. The total protein secretion and kallikrein activity decreased 20〜30% in the EXP mice between 4 and 12 weeks after the starting of experiments when compared with the CTL mice(p<0.05〜0.01).Conclusion : The results showed that the combination of exercise and diet control decreased kallikrein activity from the mice saliva, suggesting that signal transduction in the ∂-sympathetic nervous system was down regulated or the synthesis of kallikrein decreased in the salivary glands of exercise mice. The down-regulation was thought to be due to increse of 8-OHdG. This study suggested that the sufficient food intake was necessary for maintaining the oral function during physical exercise.

目的：探讨运动训练通过副交感神经系统对唾液腺的影响。材料与方法：32周龄雄性ICR小鼠，体重39 ~ 50g，随机分为3组；一个有食物限制的运动（EXP），一个没有食物限制的运动（EXA）和一个对照组（CTL）。EXP组采用自主跑轮运动，饲喂与CTL组等量的日粮（配对饲养）。匹罗卡品刺激的全唾液在配对喂养开始后15分钟、4周、8周和12周用微移液管从口腔收集。测定唾液流速、蛋白浓度、淀粉酶和钾激肽活性。-和-肾上腺素能受体刺激可分别诱发Kallikrein和淀粉酶的释放。结果：实验开始后4 ~ 12周，EXP小鼠的体重比CTL小鼠减轻约20% （p<0.05）。EXP组大鼠唾液8-OHdG显著升高。实验开始后4 ~ 12周，EXP小鼠总蛋白分泌量和钾激肽活性较CTL小鼠降低20 ~ 30% （p<0.05 ~ 0.01）。结论：运动与饮食控制相结合降低了小鼠唾液中钾激肽的活性，表明运动小鼠唾液腺中∂-交感神经系统信号转导下调或钾激肽合成减少。这种下调被认为是由于8-OHdG的增加。本研究提示，在体育锻炼过程中，充足的食物摄入是维持口腔功能的必要条件。