Effect of exercise training on salivary gland through parasympathetic nervous system

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Objective : The objective of the present study was to examine the effect of exercise training on salivary gland through parasympathetic nervous system.Materials and methods: Male ICR mice, weighing 39〜50g, at 32 weeks of age were divided into three groups; an exercise with food restriction (EXP), an exercise without food restriction (EXA), and a control (CTL). EXP group uses voluntary running wheel for exercise, and fed the same amount of diet as the CTL group (pair-feeding). The pilocarpine stimulated whole saliva was collected from the oral cavity by micro-pipet over 15 min 4, 8, and 12 weeks after beginning of the exercise with pair-feeding. The salivary flow rate, protein concentration, and amylase and kallikrein activities were determined. Kallikrein and amylase release are evoked by alpha- and beta- adrenergic receptor stimulation, respectively.Results: The body weight of EXP mice was approximately 20% less than that of CTL mice between 4 and 12 weeks after the beginning of the experiment (p<0.05). Salivary 8-OHdG was significantly increased in the EXP group. The total protein secretion and kallikrein activity decreased 20〜30% in the EXP mice between 4 and 12 weeks after the starting of experiments when compared with the CTL mice(p<0.05〜0.01).Conclusion : The results showed that the combination of exercise and diet control decreased kallikrein activity from the mice saliva, suggesting that signal transduction in the ∂-sympathetic nervous system was down regulated or the synthesis of kallikrein decreased in the salivary glands of exercise mice. The down-regulation was thought to be due to increse of 8-OHdG. This study suggested that the sufficient food intake was necessary for maintaining the oral function during physical exercise.