Classification of microorganisms and generarion of -amino butylic acid from the post-heated fermented tea

在生产过程中，对加热后的发酵茶进行采样，以研究从样品中分离出的微生物的特性。与NBRC9405^T不同，来自石口黑茶的一个真菌分离物没有类根。NBRC31005的孢子形成片状。对分离自该真菌的28S rRNA基因的D2区进行了测序，发现其与变异根霉的同源性为98.7%。对来自Awa-bancha、Ishizuchi-kurocha和Goishi-cha的循环芽孢杆菌进行了最适温度和最适pH的测定。最适温度为37~40℃，最适pH为6~7。加热后的发酵茶中的成分被发现抑制了分离物的生长。从加热后的发酵茶中分离到一株禽肠球菌和一株粪肠球菌，它们都降低了培养液的pH值，并在生长过程中增加了培养液中的乳酸含量。设计了一条扩增约1.5kbp的16S rRNA基因的引物，并对提取的DNA进行了分析。分析显示碱基序列有一定差异，用迷宫来判断γ-氨基丁酸(GABA)的学习效果，联合使用压力也能有效地判断学习效果。强制给予GABA100μg的一组大鼠在迷宫中花费的时间比给予水的一组更长。然而，当压力被组合使用时，服用GABA的小组能够最快地穿过迷宫。

Post-heated fermented teas were sampled during their production processes to study properties of microorganisms isolated from the samples. Unlike NBRC9405^T, a fungus isolate from Ishizuchi-kurocha had no rhizoid. Sporangiospores of NBRC31005 formed lamellae. The D2 region of a 28S rRNA gene from the fungus isolate was sequenced to find 98.7% homology with that of Rhizomucor variabilis.Bacillus circulans isolates from Awa-bancha, Ishizuchi-kurocha, and Goishi-cha were examined for optimum temperature and pH. The optimum temperatures were 37-40℃ and the optimum pH were 6-7. Components of the post-heated fermented teas were found to suppress the isolates in growth. Both an Enterococcus avium isolate and an Enterococcus faecium isolate from the post-heated fermented teas decreased the pH of the culture medium and increased the amount of lactic acid in the medium as they grew.DNeasy Blood & Tissue Kit by QIAGEN was used to extract DNA from the isolates. A primer was designed to multiply a 16S rRNA gene of about 1.5 kbp and used to analyze the extracted DNA. The analysis showed some differences in base sequence.A maze was used to judge the learning effect of y-amino butyric acid (GABA).Stress used in combination was also effective to judge the learning effect. A rat group given a forced administration of GABA 100 μg spent a longer time in threading the maze than a group given water. However, when the stress was used in combination, the GABA-administered group was able to thread the maze fastest.