Possibility of a new functional food component from soybeans

从大豆中提取新的功能性食品成分的可能性

• 批准号: 18500618
• 负责人: YANO Tomohiro
• 金额: $ 2.61万
• 依托单位: National Institute of Health and Nutrition
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500618/
• 关键词: food compnent form soybeans; BBI; cancer prevention foor; connexin 43

The present study was designed to investigate the effects of Bowman-Birk inhibitor concentrate(BBIC) from soybean on anti-proliferation, up-regulation of Connexin (Cx) 43 expression and inhabitation of hepatic metastasis in mice with M5076 ovarian sarcoma. M5076 ovarian sarcomas (1×10^6 cells/animal) were subcutaneously transplanted into the back of BDF_1 mice. "Pre-treated group" (n=10) and "post-treated group" (n=10) were fed with standard diet (CE-2, Crea Japan) compounded 0.5%-1% BBI from 3 weeks before and at the day of tumor inoculation until the weeks after tumor inoculation, respectively. "Control group" (n=10) was fed with CE-2 alone. The relative tumor weights in the pre- (0.013±0.010) and post- (0.012±0.013) treated groups were significantly reduced by 30.0% and 32.5% compared with that in the control group (0.040±0.022, p<0.01), respectively. The relative densities of Cx43-mRNA and Cx43 proteins in the BBIC treated groups were significantly higher than that in the control group. The median numbers of macroscopic spontaneous metastases in the pre- (1.0±2.3) and post-(1.9±3.6) treated groups were significantly lower than that in the control group (71.4±97.3), and, respectively. These results suggested that BBIC induced anti-proliferation caused by the expression of Cx43 genes in mice with M5076 ovarian sarcoma. In addition, BBIC inhibited the hepatic metastasis in M5076 bearing mice. Overall, It seems that BBI is a promising candidate to prevent the development of tumors in which Cx43 acts as a tumor suppressor gene.

本研究旨在探讨大豆Bowman-Birk抑制剂浓缩物（BBIC）对M5076卵巢肉瘤小鼠的抗增殖、上调连接蛋白（Cx） 43的表达和抑制肝转移的作用。将M5076卵巢肉瘤（1×10^6个细胞/只）皮下移植至BDF_1小鼠背部。“预处理组”（n=10）和“后处理组”（n=10）分别于肿瘤接种前3周和当日至肿瘤接种后1周，分别饲喂添加0.5% ~ 1% BBI的标准饲粮（CE-2, Crea Japan）。对照组（n=10）单独饲喂CE-2。与对照组（0.040±0.022,p<0.01）相比，治疗前（0.013±0.010）组和治疗后（0.012±0.013）组的相对肿瘤重量分别显著降低30.0%和32.5%。BBIC处理组Cx43- mrna和Cx43蛋白的相对密度显著高于对照组。治疗前（1.0±2.3）组和治疗后（1.9±3.6）组宏观自发转移中位数分别显著低于对照组（71.4±97.3）和（1.9±3.6）。上述结果表明，BBIC可诱导M5076卵巢肉瘤小鼠抗增殖，其作用机制是Cx43基因的表达。此外，BBIC还能抑制M5076荷瘤小鼠的肝转移。总的来说，BBI似乎是一个有希望的候选物，可以预防肿瘤的发展，其中Cx43作为肿瘤抑制基因。

项目成果

A New Mechanism on Anti-carcinogenic Effect of Bowman-Birk Protease Inhibitor(BBI) from Soybeans

大豆鲍曼-伯克蛋白酶抑制剂（BBI）抗癌新机制

• 发表时间: 2007
• 作者: Yano T;Saito T;Kashiwagi K;Hagiwara H;Sato H
• 通讯作者: Sato H

Negative growth control of osteosarcoma cell by Bowman-Birk protease inhibitor from soybean; involvement of connexin 43.

• DOI: 10.1016/j.canlet.2007.01.021
• 发表时间: 2007-08
• 期刊: Cancer letters
• 影响因子: 9.7
• 作者: Teruyoshi Saito;Hiromi Sato;N. Virgona;H. Hagiwara;K. Kashiwagi;Kazuyuki Suzuki;R. Asano;T. Yano

New Mechanism on Anti-carcinogenic Effect of Bowman-Birk Protease Inhibitor (BBI) from Soybeans

大豆鲍曼-伯克蛋白酶抑制剂（BBI）抗癌新机制

• 发表时间: 2007
• 作者: Yano T; Saito T; Kashiwagi K; Hagiwara H; Sato H
• 通讯作者: Sato H

がん抑制遺伝子(コネキシン43)の発現・機能回復による中皮腫発生抑制に関する研究-大豆由来のBowman-Birk protease inhibitorを用いた検討-

通过肿瘤抑制基因（连接蛋白43）的表达和功能恢复来抑制间皮瘤发展的研究 - 使用源自大豆的 Bowman-Birk 蛋白酶抑制剂的研究 -

• 发表时间: 2007
• 期刊: 大豆たん白質研究 10
• 作者: 矢野友啓;柏木維人;佐藤洋美
• 通讯作者: 佐藤洋美

The effects of Bowman-Birk Protease inhibitor concentrate BBIC) on anti-proliferation and inhbition of metastasis in M5076 bearing mice.

Bowman-Birk 蛋白酶抑制剂浓缩物 BBIC) 对携带 M5076 的小鼠的抗增殖和抑制转移的作用。

• 发表时间: 2006
• 作者: Sano Y;Yoshimura H;Nishimura Y;Sakurai N;Suzuki ;Yano T
• 通讯作者: Yano T