Possibility of a new functional food component from soybeans

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The present study was designed to investigate the effects of Bowman-Birk inhibitor concentrate(BBIC) from soybean on anti-proliferation, up-regulation of Connexin (Cx) 43 expression and inhabitation of hepatic metastasis in mice with M5076 ovarian sarcoma. M5076 ovarian sarcomas (1×10^6 cells/animal) were subcutaneously transplanted into the back of BDF_1 mice. "Pre-treated group" (n=10) and "post-treated group" (n=10) were fed with standard diet (CE-2, Crea Japan) compounded 0.5%-1% BBI from 3 weeks before and at the day of tumor inoculation until the weeks after tumor inoculation, respectively. "Control group" (n=10) was fed with CE-2 alone. The relative tumor weights in the pre- (0.013±0.010) and post- (0.012±0.013) treated groups were significantly reduced by 30.0% and 32.5% compared with that in the control group (0.040±0.022, p<0.01), respectively. The relative densities of Cx43-mRNA and Cx43 proteins in the BBIC treated groups were significantly higher than that in the control group. The median numbers of macroscopic spontaneous metastases in the pre- (1.0±2.3) and post-(1.9±3.6) treated groups were significantly lower than that in the control group (71.4±97.3), and, respectively. These results suggested that BBIC induced anti-proliferation caused by the expression of Cx43 genes in mice with M5076 ovarian sarcoma. In addition, BBIC inhibited the hepatic metastasis in M5076 bearing mice. Overall, It seems that BBI is a promising candidate to prevent the development of tumors in which Cx43 acts as a tumor suppressor gene.