Bioavailability evaluation on a new food component from soybeans

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In order to evaluate bioavailability of Bowman-birk protease inhibitor (BBI), a major anticancer agent involved in soybeans, we tried to establish a new ELISA system which can selectively BBI level in vivo with a high sensitivity. We established a new ELISA system for BBI detection, using monoclonal and polyclonal antibodies towards BBI. To improve detection sensitivity in the system, we changed several points in the system such as determination of blocking agent. As a result, the system had equal or higher detection sensitivity compared to other established protein detectionELISA systems, indicating that this system could detect BBI level in vivo.