THE ESTIMATION OF A USEFUL LEVEL OF VITAMINE INTAKE AGAINST THE PREVENTION OF LUNG ADENOCARCINOMA

估计预防肺腺癌的有效维生素摄入水平

• 批准号: 07680075
• 负责人: YANO Tomohiro
• 金额: $ 1.47万
• 依托单位: THE NATIONAL INSTITUTE OF HEALTH&NUTRITION
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680075/
• 关键词: Lung adenocarcinoma; Chemoprevention; Vitamin E; ras gene; Ornithine decarboxylase; Cell proliferation

The intake of vitmain E-supplemented diet which contained the vitamin about10-20 fold compared to control diet could significantly reduced the development of lung adenocarcinoma in mice. When this vitamin E-supplemented diet was provided, the increases of ornithine decarboxylase (ODC) activity, polyamine biosynthesis and proliferating cell nuclear antigen as markers of cell proliferation were almost suppressed. Immunohistochemical study using anti-ODC suppported viochemical results related to cell proliferation at the early stage of lung carcinogenesis. Vitamin E-dependent inhibition of prostaglandin E_2 production at an early stage of lung carcinogenesis contributed to a portion of the suppression of these events related to cell proliferation. Also, vitamin E reduced the frequency of K-ras mutation at the early stage of teh carcinogenesis which was a major event leading to the development of adenocarconoma. Overall, within the possible intake level in daily life, vitamin E could reduce the development of lung adenocarcinoma due to the regulation of cell proliferation during the carcinogenic process.

补充维生素E的饲料（维生素E含量是对照饲料的10 -20倍）可显著降低小鼠肺腺癌的发生。当提供这种维生素E补充饮食时，作为细胞增殖标志物的鸟氨酸脱羧酶（ODC）活性、多胺生物合成和增殖细胞核抗原的增加几乎被抑制。抗ODC的免疫组化研究支持肺癌发生早期与细胞增殖相关的病毒化学结果。在肺癌发生的早期阶段，维生素E依赖性抑制前列腺素E_2的产生有助于抑制这些与细胞增殖有关的事件的一部分。维生素E还可降低癌发生早期K-ras基因突变的频率，这是导致腺癌发生的主要事件。总体而言，在日常生活中可能的摄入量范围内，维生素E在致癌过程中调节细胞增殖，可减少肺腺癌的发生。

项目成果

Tomio Ichikawa: "The activation of K-ras gene at an early stage of lung tumorigenesis in mice" Canoer Letters. 107. 165-170 (1996)

Tomio Ichikawa：“K-ras 基因在小鼠肺肿瘤发生早期的激活”Canoer Letters。

Tomohiro Yano: "The inhibitory effect of vitamin E on k-ras mutation at an early stage of lung carcimogenesis in mice" European Journal Pharmacology. (in press). (1997)

Tomohiro Yano：“维生素 E 对小鼠肺癌早期阶段 k-ras 突变的抑制作用”《欧洲药理学杂志》。

Tomio Ichikawa: "The activation of k-ras gene at an early stage of lung tumorigenesis in mice" Cancer Letters. 107. 165-170 (1996)

Tomio Ichikawa：“小鼠肺肿瘤发生早期阶段 k-ras 基因的激活”《癌症快报》。

Tomohiro Yano: "Stimulating effect of excess iron feeding on spontaneous lung tumor pramotion in mice" Int. J. Vit. Nurr. Res.65. 127-130 (1995)

Tomohiro Yano：“过量铁喂养对小鼠自发性肺肿瘤进展的刺激作用” Int。

Tomio Ichikawa: "The activation of K-ras gene at ana early stage of lung tumorigenesis in mice" Cancer Letters. 107(2). 165-170 (1996)

Tomio Ichikawa：“K-ras 基因在小鼠肺部肿瘤发生早期的激活”《癌症快报》。