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STUDY ON ANTISEPTIC EFFECT OF SILVER AND COPPER IONS AGAINST LEGIONELLA IN HOT SPRING WATERS

银、铜离子对温泉水中军团菌抗菌作用的研究

基本信息

批准号：
18510035
负责人：
KATO Naoyuki
金额：
$ 2.64万
依托单位：
Toho University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18510035/
关键词：
LEGIONELLA HOT SPRINNG SILVER ION COPPER ION AMOEBA TROPHOZOITE CYST ANTISEPTIC EFFECT 殺菌作用循環装置温泉環境学

项目摘要

1. We studied the influence of chemical composition of hot springs to the antiseptic effect of silver ion.(1) The antiseptic effect of silver ion delayed according to-increases of chloriide and sulfuric acid ions in hot spring. (2) Some hot spring did not show any antiseptic effect despite enough silver ion concentration. The cause will be attributed to that silver ion formed complex with some kind of hot spring components.2. Antiseptic effect of silver ion against legionella cells in amoeba or amoeba alone was studied.(1) While legionella cells in amoeba were not killed at the concentration of 0.05 ppm of silver ion, silver ion of 2.5ppm showed an antiseptic effect. (2) Legionella cells in cyst encysted amoeba were not killed even at the concentration of 2.5ppm but did at the concentration of 5ppm. (3) Amoeba cells themselves were not killed by the concentration of-5ppm of silver ion.3. The concentration of copper ion in an aqueous solution decreased with a rise in pH.In this study … More , particles coated with copper were added into each aqueous solution with different pH at a final concentration of 0.1%and 0.5%, respectively. Eluted copper concentration was different in 0.1% and 0.5%, however there was no difference among different pH in dissolution rate of copper along with the time course. 4. Antiseptic effect of copper ion against legionella cells in amoeba or amoeba alone was studied.(1) The antiseptic duct of copper ion against legionella cells m trophozoite cells was not shown at the concentration of 1ppm, while legionella cells of 76% at 5ppm and 95% at 10ppm were killed, respectively. (2) The legionella cell in cyst form were not killed at the concentration of 1ppm of copper ion as well as in trophozoite cell. Eighty fie % and 94% of legionella cells were killed at the concentration of 5ppm and 10ppm, respectively. (3) Seventy four % and 47% of amoeba cells survived in the copper ion concentration of 5ppm and 10ppm, respectively.5. In the experiment of antiseptic effect against legionella in copper ion solution with pH7.2, Legionella cells were completely killed at 6h after the addition of 0.5 ppm copper ion. On the other hand, each 0.1% copper solution adjusted. to pH6.0, 7.4 and 8.5 showed all antiseptic effect, and distilled water, tap water and marine water adjusted to copper concentration of 0.1% had also the same effect. The antiseptic effect of hot spring waters against legionella was diversed among each hot spring due to the differences of pH and chemical composition of hot springs. Less

1.研究了温泉的化学成分对银离子防腐效果的影响。(1)银离子的防腐作用随温泉中氯离子和硫酸根离子的增加而延迟。 (2)有些温泉即使银离子浓度足够，也没有显示出任何防腐作用。其原因可能是银离子与某些温泉成分形成了络合物.研究了银离子对阿米巴或单独阿米巴体内军团菌的抗菌作用。(1)当银离子浓度为0.05 ppm时，阿米巴体内的军团菌细胞不能被杀死，而银离子浓度为2.5 ppm时，显示出杀菌作用。(2)在2.5ppm浓度下对包囊变形虫体内的军团菌细胞也无杀灭作用，而在5 ppm浓度下对军团菌细胞有杀灭作用。(3)阿米巴细胞本身不被浓度为-5ppm的银离子杀死.水溶液中铜离子的浓度随着pH的升高而降低。 ...更多信息将铜包覆的颗粒分别以0.1%和0.5%的终浓度添加到具有不同pH的每种水溶液中。洗脱铜浓度在0.1%和0.5%范围内不同，但铜的溶出率在不同pH值下随时间的变化沿着无差异。4.研究了铜离子对阿米巴和单独阿米巴体内军团菌的抗菌作用。(1)铜离子浓度为1 ppm时，对滋养体细胞内军团菌无杀菌作用，而5 ppm和10 ppm时对军团菌的杀灭率分别为76%和95%。 (2)1 ppm铜离子不能杀死包囊状军团菌细胞和滋养体细胞。在5 ppm和10 ppm浓度下，对军团菌的杀灭率分别为85%和94%。 (3)在铜离子浓度为5 ppm和10 ppm时，阿米巴细胞存活率分别为74%和47%.在pH7.2的铜离子溶液中对军团菌的杀菌效果试验中，加入0.5ppm铜离子后6 h军团菌即被完全杀灭。另一方面，调整各0.1%铜溶液。pH值为6.0、7.4和8.5时，均能达到防腐效果，蒸馏水、自来水和海水中铜浓度为0.1%时，也能达到防腐效果。各温泉沃茨对军团菌的抑菌效果因温泉的pH值和化学成分的不同而存在差异。少