Improvement of sensitivity for chemiluminescnt detection system using complex

研究了三（2，2 ′-联吡啶）钌（Ⅲ）与各种化合物的荧光反应。五元杂芳族化合物产生强烈的发光。荧光强度随芳环电子密度的增加而增加。在α-位上具有羧基的脂肪族仲胺显示非常强的化学发光。研究了三（2，2 ′-联吡啶）钌（Ⅲ）与2，5-二甲基噻吩的荧光反应。三（2，21-联吡啶）钌（III）与2，5-二甲基噻吩以2：1的比例反应。反应产物经鉴定为5-甲基-2-噻吩甲醛。通过同时测量荧光强度和氧化电位，并结合前人对噻吩衍生物氧化反应的研究，推测了涉及去质子化的荧光反应机理.由于人体体液中的亚氨基酸是各种疾病的重要生物标志物， ...更多信息 建立了同时测定人血清中亚氨基酸的方法。为了开发新的共反应物用于电化学发光免疫分析，对三（2，2 '-联吡啶）钌（II）电化学发光检测体系进行了初步研究。结果表明，三（2，2 ′-联吡啶）钌（II）/ N，N-二丙基-L-丙氨酸体系的灵敏度与目前广泛应用的三（2，2 ′-联吡啶）钌（II）/三丙胺体系相当。由于N，N-二丙基-L-丙氨酸在常温下为固体，其水溶液为中性，因此与三丙胺相比易于使用。合成了一种新的衍生化试剂2，4，5-三甲基-3-噻吩基乙酸，用于电生三（2，2 '-联吡啶）钌（III）的荧光检测。十三烷基酚和双酚A的混合溶液中，可以检测到ppb级的使用高效液相色谱。建立了环境水样中磷酸盐的灵敏测定方法。在硫酸中，磷酸盐与乙醇反应生成的磷钼酸盐与脂肪族叔胺形成离子对。磷酸根是通过三（2，2 '-联吡啶）钌（III）荧光检测离子对中的脂肪族叔胺来间接测定的。少

The chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) with various compounds was examined. Five-membered heteroaromatic compounds produced intense emission. The chemiluminescent intensity increased with an increase in electron density of the aromatic ring. Aliphatic secondary amines having a carboxyl group at the α-position showed very intense chemiluminescence. The chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) with 2,5-dimethylthiophene was examined in detail. Tris(2,21-bipyridine)ruthenium(III) reacted with 2,5-dimethylthiophene in the ratio of 2:1. The reaction product was identified as 5-methyl-2-thiophenecarbaldeyde. From the simultaneous measurement of the chemiluminescent intensity and the oxidation potential and previously reported studies on the oxidative reaction of thiophene derivatives, the chemiluminescent reaction mechanism involving deprotonation was estimated. Since imino acids in human body fluid are important biomakers for various ill … More nesses, a sensitive simultaneous determination method for imino acids in human serum was developed. Four imino acids were successfully determined within 60 min.To develop novel co-reactants for electrochemiluminesecence (ECL) immunoassays, preliminary studies on the tris(2,2'-bipyridine)ruthenium(II) ECL detection system using some compounds as co-reactants were performed. It was suggested that the sensitivity of the tris(2,2'-bipyridine)ruthenium(II)/ N,N-dipropyl-L-alanine system was comparable to that of the tris(2,2'-bipyridine)ruthenium(II)/ tripropylamine system, which has been widely used. Since N,N-dipropyl-L-alanine is a solid at ordinary temperature and its aqueous solution is neutral, it is ease of use compared to tripropylamine. A novel derivatization reagent, (2,4,5-trimethyl-3-thieny)acetic acid, for electrogenerated tris(2,2'-bipyridine)ruthenium (III) chemiluminescent detection was synthesized. Thirteen alkylphenols and bisphenol A in a mixed solution could be detected at ppb levels using high performance liquid chromatography. A sensitive determination method of phosphate in environmental water was developed. Molybdophosphate produced by the reaction of phosphate with molybdate in sulfuric acid formed an ion-pair with aliphatic tertiary amines. Phosphate was indirectly determined by the measurement of the aliphatic tertiary amines in the ion-pairs with tris(2,2'-bipyridine)ruthenium(III) chemiluminescent detection. Less