Application of bioreactors for culture of differentiated cells and solid-phase tissues

该项目的目的是开发在体外生产三维人体软骨的方法。组织工程软骨有一系列的应用，包括毒性测试，用于生产治疗剂，以及作为外科移植设备。生物反应器将用于在受控条件下培养软骨，以开发具有尽可能接近天然关节软骨特性的活组织。新的培养策略将用于提高生长因子的可用性，并提供足够的氧气和营养交换。这些技术有可能在工程软骨的质量方面产生显着的改善。

The aim of this project is to develop methods for producing three-dimensional human cartilage outside of the body. Tissue-engineered cartilage has a range of applications, including in toxicity testing, for production of therapeutics, and as surgical transplant devices. Bioreactors will be used to culture cartilage under controlled conditions for development of living tissues with properties as close as possible to those of native articular cartilage. Novel culture strategies will be used to enhance the availability of growth factors and provide adequate oxygen and nutrient exchange. These techniques have the potential to yield significant improvements in the quality of engineered cartilage.